Reviewed, UniProtKB/Swiss-Prot A2XNT2 (CESA5_ORYSI)

Names and origin

Protein names

Recommended name:
    Probable cellulose synthase A catalytic subunit 5 [UDP-forming]
    EC=2.4.1.12
Alternative name(s):
    OsCesA5

Gene names

Name:	CESA5
ORF Names:	OsI_013725

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	1092 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1092	1092	Probable cellulose synthase A catalytic subunit 5 [UDP-forming]		PRO_0000319364
Regions
	Topological domain	1 – 279	279	Cytoplasmic Potential
	Transmembrane	280 – 300	21	Potential
	Topological domain	301 – 302	2	Extracellular Potential
	Transmembrane	303 – 323	21	Potential
	Topological domain	324 – 868	545	Cytoplasmic Potential
	Transmembrane	869 – 889	21	Potential
	Topological domain	890 – 901	12	Extracellular Potential
	Transmembrane	902 – 922	21	Potential
	Topological domain	923 – 938	16	Cytoplasmic Potential
	Transmembrane	939 – 959	21	Potential
	Topological domain	960 – 987	28	Extracellular Potential
	Transmembrane	988 – 1008	21	Potential
	Topological domain	1009 – 1019	11	Cytoplasmic Potential
	Transmembrane	1020 – 1040	21	Potential
	Topological domain	1041 – 1049	9	Extracellular Potential
	Transmembrane	1050 – 1070	21	Potential
	Topological domain	1071 – 1092	22	Cytoplasmic Potential
	Zinc finger	39 – 85	47	RING-type; degenerate
	Coiled coil	450 – 479	30	Potential
Sites
	Active site	398	1	Potential
	Active site	792	1	Potential
	Metal binding	39	1	Zinc 1 By similarity
	Metal binding	42	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	61	1	Zinc 2 By similarity
	Metal binding	66	1	Zinc 1 By similarity
	Metal binding	69	1	Zinc 1 By similarity
	Metal binding	81	1	Zinc 2 By similarity
	Metal binding	84	1	Zinc 2 By similarity
	Binding site	564	1	Substrate Potential
	Binding site	566	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	A2XNT2-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: A682D0A434BE24D0	FASTA	1,092	123,396
	10 20 30 40 50 60 MEASAGLVAG SHNRNELVVI RRDGEPGPKP VKHTNGQVCQ ICGDDVGLTP DGEPFVACNE 70 80 90 100 110 120 CAFPVCRDCY EYERREGTQN CPQCKTRFKR LKGCARVPGD EEEEDVDDLE NEFNWRDKTD 130 140 150 160 170 180 SQYVAESMLH GHMSYGRGGD LDGVPQHFQP IPNVPLLTNG EMADDIPPEQ HALVPSFMGG 190 200 210 220 230 240 GGKRIHPLPY ADPNLPVQPR SMDPSKDLAA YGYGSVAWKE RMESWKQKQE RLHQMRNDGG 250 260 270 280 290 300 GKDWDGDGDD ADLPLMDEAR QPLSRKIPIS SSLVNPYRMI IIIRLVVLGF FFHYRVMHPV 310 320 330 340 350 360 PDAFALWLIS VICEIWFAMS WILDQFPKWF PIERETYLDR LTLRFDKEGQ QSQLAPVDFF 370 380 390 400 410 420 VSTVDPMKEP PLVTANTVLS ILAVDYPVDK VSCYVSDDGA AMLTFEALSE TSEFAKKWVP 430 440 450 460 470 480 FCKRYSLEPR APEWYFQQKI DYLKDKVAPN FVRERRAMKR EYEEFKVRIN ALVAKAQKVP 490 500 510 520 530 540 EEGWTMQDGT PWPGNNVRDH PGMIQVFLGQ SGGHDVEGNE LPRLVYVSRE KRPGYNHHKK 550 560 570 580 590 600 AGAMNALVRV SAVLTNAPYM LNLDCDHYIN NSKAIKEAMC FMMDPLVGKK VCYVQFPQRF 610 620 630 640 650 660 DGIDRHDRYA NRNVVFFDIN MKGLDGIQGP IYVGTGCVFR RQALYGYDAP KSKKPPSRTC 670 680 690 700 710 720 NCWPKWCICC CCFGNRTNKK KTAKPKTEKK KRLFFKRAEN QSPAYALGEI DEGAPGAENE 730 740 750 760 770 780 KAGIVNQQKL EKKFGQSSVF VASTLLENGG TLKSASPASL LKEAIHVISC GYEDKTDWGK 790 800 810 820 830 840 EIGWIYGSVT EDILTGFKMH CHGWRSIYCI PKRAAFKGSA PLNLSDRLHQ VLRWALGSIE 850 860 870 880 890 900 IFFSNHCPLW YGYGGGLKCL ERFSYINSIV YPWTSIPLLA YCTLPAICLL TGKFITPELT 910 920 930 940 950 960 NIASLWFMSL FICIFATGIL EMRWSGVGID DWWRNEQFWV IGGVSSHLFA VFQGLLKVIA 970 980 990 1000 1010 1020 GIDTSFTVTS KGGDDEEFSE LYTFKWTTLL IPPTTLLLLN FIGVVAGVSN AINNGYESWG 1030 1040 1050 1060 1070 1080 PLFGKLFFAF WVIVHLYPFL KGLVGRQNRT PTIVIVWSIL LASIFSLLWV RIDPFLAKND 1090 GPLLEECGLD CN

References

[1]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000128 Genomic DNA. Translation: EAY92492.1.
3D structure databases
SMR	A2XNT2. Positions 24-97.
ModBase	Search...
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CESA5_ORYSI

Accession

Primary (citable) accession number: A2XNT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 20, 2007
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)