A2XN66 (CESA2_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cellulose synthase A catalytic subunit 2 [UDP-forming]
EC=2.4.1.12

Alternative name(s):
OsCesA2

Gene names

Name:	CESA2
ORF Names:	OsI_013509

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	1073 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1073	1073	Probable cellulose synthase A catalytic subunit 2 [UDP-forming]		PRO_0000319359
Regions
	Topological domain	1 – 270	270	Cytoplasmic Potential
	Transmembrane	271 – 291	21	Helical; Potential
	Topological domain	292 – 293	2	Extracellular Potential
	Transmembrane	294 – 314	21	Helical; Potential
	Topological domain	315 – 856	542	Cytoplasmic Potential
	Transmembrane	857 – 877	21	Helical; Potential
	Topological domain	878 – 882	5	Extracellular Potential
	Transmembrane	883 – 903	21	Helical; Potential
	Topological domain	904 – 918	15	Cytoplasmic Potential
	Transmembrane	919 – 939	21	Helical; Potential
	Topological domain	940 – 969	30	Extracellular Potential
	Transmembrane	970 – 990	21	Helical; Potential
	Topological domain	991 – 1001	11	Cytoplasmic Potential
	Transmembrane	1002 – 1022	21	Helical; Potential
	Topological domain	1023 – 1031	9	Extracellular Potential
	Transmembrane	1032 – 1052	21	Helical; Potential
	Topological domain	1053 – 1073	21	Cytoplasmic Potential
	Zinc finger	13 – 59	47	RING-type; degenerate
	Coiled coil	443 – 470	28	Potential
Sites
	Active site	389	1	Potential
	Active site	773	1	Potential
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	16	1	Zinc 1 By similarity
	Metal binding	32	1	Zinc 2 By similarity
	Metal binding	35	1	Zinc 2 By similarity
	Metal binding	40	1	Zinc 1 By similarity
	Metal binding	43	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 2 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Binding site	555	1	Substrate Potential
	Binding site	557	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	A2XN66 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 55B677033E77A7BE	FASTA	1,073	120,682
	10 20 30 40 50 60 MDGAKSGKQC HVCQICGDGV GTAADGELFT ACDVCGFPVC RPCYEYERKD GSQACPQCKT 70 80 90 100 110 120 KYKRHKGSPP ILGDESDDVD ADDASDVNYP TSGNQDHKHK IAERMLTWRM NSGRNDDIVH 130 140 150 160 170 180 SKYDSGEIGH PKYDSGEIPR IYIPSLTHSQ ISGEIPGASP DHMMSPVGNI GRRGHPFPYV 190 200 210 220 230 240 NHSPNPSREF SGSLGNVAWK ERVDGWKMKD KGAIPMANGT SIAPSEGRGV GDIDASTDYN 250 260 270 280 290 300 MEDALLNDET RQPLSRKVPI SSSRINPYRM VIVLRLIVLC IFLHYRITNP VRNAYPLWLL 310 320 330 340 350 360 SVICEIWFAL SWILDQFPKW SPINRETYLD RLALRYDREG EPSQLAPVDI FVSTVDPMKE 370 380 390 400 410 420 PPLVTANTVL SILAVDYPVD KVSCYVSDDG AAMLTFDALA ETSEFARKWV PFCKKYSIEP 430 440 450 460 470 480 RAPEWYFAQK IDYLKDKVQA SFVKDRRAMK REYEEFKVRV NALVAKAQKV PEEGWIMQDG 490 500 510 520 530 540 TPWPGNNTRD HPGMIQVFLG HSGGLDTEGN ELPRLVYVSR EKRPGFQHHK KAGAMNALVR 550 560 570 580 590 600 VSAVLTNGQY LLNLDCDHYI NNSKALREAM CFLMDPNLGR RVCYVQFPQR FDGIDRNDRY 610 620 630 640 650 660 ANRNTVFFDI NLRGLDGLQG PVYVGTGCVF NRTALYGYEP PIKQKRPGYF SSLCGGRKKT 670 680 690 700 710 720 KKSKEKSTEK KKSHKHVDSS VPVFNLEDIE EGIEGSGFDD EKSLLMSQMS LEKRFGQSSV 730 740 750 760 770 780 FVASTLMEYG GVPQSATPES LLKEAIHVIS CGYEDKSDWG TEIGWIYGSV TEDILTGFKM 790 800 810 820 830 840 HARGWRSIYC MPKRPAFKGS APINLSDRLN QVLRWALGSV EILFSRHCPI WYGYGGRLKF 850 860 870 880 890 900 LERFAYINTT IYPLTSIPLL LYCILPAICL LTGKFIIPEI SNFASIWFIS LFLSIFATGI 910 920 930 940 950 960 LEMRWSGVGI DEWWRNEQFW VIGGISAHLF AVFQGLLKVL AGIDTSFTVT SKASDEEGDF 970 980 990 1000 1010 1020 AELYMFKWTT LLIPPTTILI INLVGVVAGI SYAINSGYQS WGPLFGKLFF AFWVIVHLYP 1030 1040 1050 1060 1070 FLKGLMGRQN RTPTIVVVWA ILLASIFSLL WVRIDPFTTR VTGPDTQKCG INC

References

[1]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000128 Genomic DNA. Translation: EAY92276.1.
3D structure databases
ProteinModelPortal	A2XN66.
SMR	A2XN66. Positions 13-71.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g59340.1; LOC_Os03g59340.1; LOC_Os03g59340.
Phylogenomic databases
GeneTree	EPGT00070000027964.
HOGENOM	HBG744549.
PhylomeDB	A2XN66.
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CESA2_ORYSI

Accession

Primary (citable) accession number: A2XN66

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 20, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program