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A2XFC8 (MPK5_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 5

Short name=MAP kinase 5
EC=2.7.11.24
Alternative name(s):
Benzothiadiazole-induced MAP kinase 1
MAP kinase 2
Multiple stress-responsive MAP kinase 2
OsBIMK1
OsMAP1
OsMAPK2
OsMAPK5
OsMPK3
OsMSRMK2
Gene names
Name:MPK5
Synonyms:BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
ORF Names:OsI_010771
OrganismOryza sativa subsp. indica (Rice)
Taxonomic identifier39946 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in disease resistance and abiotic stress tolerance signaling pathways By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Subunit structure

Interacts with MKK1. Ref.4

Induction

By benzothiadiazole (BTH), dichloroisonicotinic acid, probenazole, jasmonic acid, wounding and infection with P.syringae and M.grisea. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-194 and Tyr-196, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processPlant defense
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processactivation of MAPK activity involved in osmosensory signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

camalexin biosynthetic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

inflorescence development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

ovule development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

priming of cellular response to stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of stomatal complex development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of stomatal complex patterning

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to UV-B

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to bacterium

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to chitin

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to osmotic stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to oxidative stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to wounding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Mitogen-activated protein kinase 5
PRO_0000300870

Regions

Domain36 – 322287Protein kinase
Nucleotide binding42 – 509ATP By similarity
Motif194 – 1963TXY

Sites

Active site1621Proton acceptor By similarity
Binding site651ATP By similarity

Amino acid modifications

Modified residue1941Phosphothreonine By similarity
Modified residue1961Phosphotyrosine By similarity

Experimental info

Sequence conflict3401F → I in ABH01189. Ref.2
Sequence conflict3671I → F in AAK01710. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A2XFC8 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: 417D81732635F2D3

FASTA36942,995
        10         20         30         40         50         60 
MDGAPVAEFR PTMTHGGRYL LYDIFGNKFE VTNKYQPPIM PIGRGAYGIV CSVMNFETRE 

        70         80         90        100        110        120 
MVAIKKIANA FNNDMDAKRT LREIKLLRHL DHENIIGIRD VIPPPIPQAF NDVYIATELM 

       130        140        150        160        170        180 
DTDLHHIIRS NQELSEEHCQ YFLYQILRGL KYIHSANVIH RDLKPSNLLL NANCDLKICD 

       190        200        210        220        230        240 
FGLARPSSES DMMTEYVVTR WYRAPELLLN STDYSAAIDV WSVGCIFMEL INRQPLFPGR 

       250        260        270        280        290        300 
DHMHQMRLIT EVIGTPTDDE LGFIRNEDAR KYMRHLPQYP RRTFASMFPR VQPAALDLIE 

       310        320        330        340        350        360 
RMLTFNPLQR ITVEEALDHP YLERLHDIAD EPICLEPFSF DFEQKALNED QMKQLIFNEA 


IEMNPNIRY 

« Hide

References

« Hide 'large scale' references
[1]"OsBIMK1, a rice MAP kinase gene involved in disease resistance responses."
Song F., Goodman R.M.
Planta 215:997-1005(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
Strain: cv. Yuanfengzao.
[2]"Oryza sativa (indica cultivar-group) mitogen activated protein kinase 3 (MPK3) mRNA."
Rao K.P., Kumar K., Sharma P., Sinha A.K.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Pusa Basmati.
[3]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. 93-11.
[4]"Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
Wen J.-Q., Oono K., Imai R.
Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK1.
[5]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF332873 mRNA. Translation: AAK01710.1.
DQ826422 mRNA. Translation: ABH01189.1.
CM000128 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalA2XFC8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os03g17700.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneA2XFC8.

Phylogenomic databases

eggNOGCOG0515.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK5_ORYSI
AccessionPrimary (citable) accession number: A2XFC8
Secondary accession number(s): Q0PIU7 expand/collapse secondary AC list , Q7FNE2, Q8GZZ3, Q8S3T6, Q9AXF2, Q9FQM3, Q9FSE6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families