Reviewed, UniProtKB/Swiss-Prot A2XFC7 (APX1_ORYSI)

Names and origin

Protein names

Recommended name:
    L-ascorbate peroxidase 1, cytosolic
      Short name=APXa
    EC=1.11.1.11
Alternative name(s):
    OsAPx01

Gene names

Name:	APX1
ORF Names:	OsI_010770

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in hydrogen peroxide removal By similarity.
Catalytic activity	L-ascorbate + H(2)O(2) = dehydroascorbate + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group By similarity. Binds 1 potassium or calcium ion per subunit By similarity.
Subcellular location	CytoplasmProbable.
Induction	By salt stress and hydrogen peroxide.
Sequence similarities	Belongs to the peroxidase family. Ascorbate peroxidase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide Stress response
Cellular component	Cytoplasm
Ligand	Calcium Heme Iron Metal-binding Potassium
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 250	249	L-ascorbate peroxidase 1, cytosolic		PRO_0000300257
Sites
	Active site	42	1	Proton acceptor By similarity
	Metal binding	163	1	Iron (heme axial ligand) By similarity
	Metal binding	164	1	Potassium or calcium By similarity
	Metal binding	180	1	Potassium or calcium By similarity
	Metal binding	182	1	Potassium or calcium By similarity
	Metal binding	187	1	Potassium or calcium By similarity
	Site	38	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	218	1	C → R in AAP13093. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A2XFC7-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 7867C10191CC92E9	FASTA	250	27,103
	10 20 30 40 50 60 MAKNYPVVSA EYQEAVEKAR QKLRALIAEK SCAPLMLRLA WHSAGTFDVS SKTGGPFGTM 70 80 90 100 110 120 KTPAELSHAA NAGLDIAVRM LEPIKEEIPT ISYADFYQLA GVVAVEVSGG PAVPFHPGRE 130 140 150 160 170 180 DKPAPPPEGR LPDATKGSDH LRQVFGAQMG LSDQDIVALS GGHTLGRCHK ERSGFEGPWT 190 200 210 220 230 240 RNPLQFDNSY FTELLSGDKE GLLQLPSDKA LLSDPAFCPL VEKYAADEKA FFEDYKEAHL 250 KLSELGFADA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of ascorbate peroxidase gene from indica rice, Khao Dawk Mali 105." Duriyapong F., Peyachoknagul S., Apisitwanich S. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Khao Dawk Mali 105.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.
[3]	"Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome." Teixeira F.K., Menezes-Benavente L., Margis R., Margis-Pinheiro M. J. Mol. Evol. 59:761-770(2004) [PubMed: 15599508] [Abstract] Cited for: NOMENCLATURE.
[4]	"Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2." Tsai Y.-C., Hong C.-Y., Liu L.-F., Kao C.H. J. Plant Physiol. 162:291-299(2005) [PubMed: 15832681] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY254495 mRNA. Translation: AAP13093.1. CM000128 Genomic DNA. Translation: EAY89537.1.
3D structure databases
HSSP	HSSP built from PDB template 1OAF based on UniProtKB Q43758.
SMR	A2XFC7. Positions 2-250.
ModBase	Search...
Organism-specific databases
Gramene	P93404.
Family and domain databases
InterPro	IPR002207. Asc_perxdse. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00459. ASPEROXIDASE. PR00458. PEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APX1_ORYSI

Accession

Primary (citable) accession number: A2XFC7
Secondary accession number(s): P93404, Q84QS3, Q8GZZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 20, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)