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Protein

Fructose-1,6-bisphosphatase, chloroplastic

Gene

OsI_10887

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible reaction from fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate, to regenerate the primary CO2 acceptor molecule, ribulose-1,5-bisphosphate (Probable). Involved in the regulation of photosynthetic performance and sucrose synthesis (Ref. 2).1 Publication1 Publication

Miscellaneous

In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.Curated

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by sodium chloride.1 Publication

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.Curated
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi128Magnesium 1By similarity1
Metal bindingi157Magnesium 1By similarity1
Metal bindingi157Magnesium 2By similarity1
Metal bindingi178Magnesium 2By similarity1
Metal bindingi178Magnesium 3By similarity1
Metal bindingi180Magnesium 2; via carbonyl oxygenBy similarity1
Metal bindingi181Magnesium 3By similarity1
Binding sitei286SubstrateBy similarity1
Binding sitei318SubstrateBy similarity1
Binding sitei336SubstrateBy similarity1
Binding sitei338SubstrateBy similarity1
Binding sitei348SubstrateBy similarity1
Metal bindingi354Magnesium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCalvin cycle, Carbohydrate metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphatase, chloroplasticCurated (EC:3.1.3.111 Publication)
Short name:
FBPaseCurated
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolaseCurated
Gene namesi
ORF Names:OsI_10887Imported, OsI_10888Imported
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000007015 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47ChloroplastSequence analysisAdd BLAST47
ChainiPRO_000043873448 – 406Fructose-1,6-bisphosphatase, chloroplasticAdd BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi222 ↔ 227Redox-active (light-modulated)By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Light activation through pH changes, Mg2+ levels and also by light-modulated reduction of essential disulfide groups via the ferredoxin-thioredoxin f system.By similarity

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi39946.BGIOSGA011043-PA.

Structurei

3D structure databases

ProteinModelPortaliA2XEX2.
SMRiA2XEX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 184Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the FBPase class 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1458. Eukaryota.
COG0158. LUCA.
HOGENOMiHOG000191265.
OMAiCMYSSST.
OrthoDBiEOG09360D1I.

Family and domain databases

CDDicd00354. FBPase. 1 hit.
HAMAPiMF_01855. FBPase_class1. 1 hit.
InterProiView protein in InterPro
IPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiView protein in Pfam
PF00316. FBPase. 1 hit.
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiView protein in PROSITE
PS00124. FBPASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2XEX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAATTSSH LLLLSRQQAA ASLQCGLSFR RQPGRLAGGS SAPSVRCMAA
60 70 80 90 100
VDTASAPAAT EASKKSSYEI TTLTTWLLKQ EQAGTIDGEM TIVLASISTA
110 120 130 140 150
CKQIASLVQR APISNLTGVQ GAVNVQGEDQ KKLDVVSNEV FSNCLKSSGR
160 170 180 190 200
TGVIASEEED VPVAVEESYS GNYIVVFDPL DGSSNIDAAV STGSIFGIYS
210 220 230 240 250
PNDECLADIA DDQNLDQVEQ RCIVSVCQPG SNLLAAGYCM YSSSVIFVLT
260 270 280 290 300
IGTGVYVFTL DPMYGEFVLT QEKVQIPKAG KIYAFNEGNY ALWDDKLKSY
310 320 330 340 350
MDSLKEPGPS GKPYSARYIG SLVGDFHRTL LYGGIYGYPR DQKSKNGKLR
360 370 380 390 400
LLYECAPMSF IVEQAGGKGS DGHQRILDIM PTEIHQRVPL YIGSVEEVEK

VEKFLA
Length:406
Mass (Da):43,604
Last modified:March 20, 2007 - v1
Checksum:i1665BB680EC06069
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti343K → R in ABY75186 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000128 Genomic DNA. Translation: EAY89382.1.
CM000128 Genomic DNA. Translation: EAY89383.1.
EU370973 mRNA. Translation: ABY75186.1.

Genome annotation databases

EnsemblPlantsiBGIOSGA011042-TA; BGIOSGA011042-PA; BGIOSGA011042.
BGIOSGA011043-TA; BGIOSGA011043-PA; BGIOSGA011043.
OS03T0267300-01; OS03T0267300-01; OS03G0267300.
GrameneiBGIOSGA011042-TA; BGIOSGA011042-PA; BGIOSGA011042.
BGIOSGA011043-TA; BGIOSGA011043-PA; BGIOSGA011043.
OS03T0267300-01; OS03T0267300-01; OS03G0267300.

Similar proteinsi

Entry informationi

Entry nameiF16P1_ORYSI
AccessioniPrimary (citable) accession number: A2XEX2
Secondary accession number(s): B0LSR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 18, 2017
Last sequence update: March 20, 2007
Last modified: November 22, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families