Reviewed, UniProtKB/Swiss-Prot A2X8W3 (HMDH1_ORYSI)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
      Short name=HMG-CoA reductase 1
    EC=1.1.1.34

Gene names

Name:	HMG1
ORF Names:	OsI_008506

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.
Sequence caution	The sequence AAA21720.1 differs from that shown. Reason: Frameshift at positions 200, 206, 477 and 482.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1		PRO_0000295657
Regions
	Transmembrane	63 – 83	21	Potential
	Transmembrane	496 – 516	21	Potential
	Region	84 – 124	41	Linker By similarity
	Region	125 – 539	415	Catalytic By similarity
Sites
	Active site	218	1	Charge relay system By similarity
	Active site	350	1	Charge relay system By similarity
	Active site	426	1	Charge relay system By similarity
	Active site	524	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
	Glycosylation	528	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	48	1	L → V in AAA21720. Ref.1
	Sequence conflict	104 – 108	5	RPAAA → APP in AAA21720. Ref.1
	Sequence conflict	123	1	A → G in AAA21720. Ref.1
	Sequence conflict	257 – 258	2	SA → CP in AAA21720. Ref.1
	Sequence conflict	444	1	D → N in AAA21720. Ref.1
	Sequence conflict	494	1	K → G in AAA21720. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A2X8W3-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 031F08C2671B910E	FASTA	539	56,331
	10 20 30 40 50 60 MDVRRGGGGG RIVGAARRAL TWGALPLPMR ITNGLAMVSL VLSSCDLLRL CSDRERPLGG 70 80 90 100 110 120 REFATVVCQL ASVVYLLSLF AHPDAPATTT GDDDDGQGGS RRARPAAAEP APMHGHGGGM 130 140 150 160 170 180 MEADDEEIVA AVASGALPSH RLESRLGDCR RAARLRREAL RRVTGRGVEG LPFDGMDYQA 190 200 210 220 230 240 ILGQCCEMPV GYVQLPVGVA GPLLLDGREY HVPMATTEGC LVASVNRGCR AISASGGAFS 250 260 270 280 290 300 VLLRDAMSRA PAVKLPSAMR AAELKAFAEA PANFELLAAV FNRSSRFGRL QDIRCALAGR 310 320 330 340 350 360 NLYMRFSCIT GDAMGMNMVS KGVENVLGYL QNVFPDMDVI SVSGNYCSDK KPTAVNWIEG 370 380 390 400 410 420 RGKSVVCEAI IKGDVVQKVL KTTVEKLVEL NIIKNLAGSA VAGALGGFNA HASNIVTALF 430 440 450 460 470 480 IATGQDPAQN VESSQCITML EEVDDGDDLH ISVTMPSIEV GTIGGGTCLA SQAACLNLLG 490 500 510 520 530 VKGSNHGSPG ANAKRLATIV AGSVLAGELS LLAALASGHL VKSHMMYNRS SKDVAKAAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a monocot 3-hydroxy-3-methylglutaryl coenzyme A reductase gene that is elicitor-inducible." Nelson A.J., Doerner P.W., Zhu Q., Lamb C.J. Plant Mol. Biol. 25:401-412(1994) [PubMed: 8049366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. IR36.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L28995 Genomic DNA. Translation: AAA21720.1. Frameshift. CM000127 Genomic DNA. Translation: EAY87273.1. Different initiation.
PIR	S46314.
3D structure databases
HSSP	HSSP built from PDB template 1DQA based on UniProtKB P04035.
ModBase	Search...
Organism-specific databases
Gramene	P48019.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH1_ORYSI

Accession

Primary (citable) accession number: A2X8W3
Secondary accession number(s): P48019

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	November 25, 2008
This is version 16 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)