A2X5P7 (INV1_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 1
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 1
Invertase 1
OsCIN1
Sucrose hydrolase 1

Gene names

Name:	CIN1
ORF Names:	OsI_007390

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in sucrose partitioning during seed development and in stress response By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	nectar secretion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to wounding Inferred from electronic annotation. Source: EnsemblPlants/Gramene starch metabolic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	inulinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene levanase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 577	555	Beta-fructofuranosidase, insoluble isoenzyme 1		PRO_0000303010
Sites
	Active site	63	1	By similarity
Amino acid modifications
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	212	1	R → L in AAQ24869. Ref.1
	Sequence conflict	375	1	K → T in AAQ24869. Ref.1
	Sequence conflict	384	1	S → F in AAQ24869. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A2X5P7 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 6441FA23D4E3170E	FASTA	577	63,994
	10 20 30 40 50 60 MGTRLLALAP WLLLLLLQLA GASHVVHRSL EAEQAPSSVP ASIVSPLLRT GYHFQPPMNW 70 80 90 100 110 120 INDPNGPLYY KGWYHLFYQY NPKGAVWGNI VWAHSVSQDL INWIALEPAI KPDIPSDQYG 130 140 150 160 170 180 CWSGSATILP DGTPAILYTG IDRPNINYQV QNIAFPKNAS DPLLREWVKP AYNPVATPEP 190 200 210 220 230 240 GMNATQFRDP TTAWYADGHW RMLVGGLKGA RRGLAYLYRS RDFKTWVRAK HPLHSALTGM 250 260 270 280 290 300 WECPDFFPLQ APGLQAGLDT SVPSSKYVLK NSLDLTRYDY YTVGIYNKVT ERYVPDNPAG 310 320 330 340 350 360 DYHRLRYDYG NFYASKTFFD PVKHRRILLG WANESDSVTY DKAKGWAGIH AIPRKVWLDP 370 380 390 400 410 420 SGKQLLQWPI EELEKLRGKS VSVSDKVVKP GEHFQVTGLG TYQADVEVSL EVSGLEKAEA 430 440 450 460 470 480 LDPAFGDDAE RLCGAKGADV RGGVVFGLWV LASAGLEEKT AVFFRVFKPA GHGAKPVVLM 490 500 510 520 530 540 CTDPTKSSLS PDLYKPTFAG FVDTDISSGK ISLRSLIDRS VVESFGAGGK TCILSRVYPS 550 560 570 MAIGDKAHLY VFNNGEADIK ISHLKAWEMK KPLMNGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of three rice cell wall invertase genes." Wang Y.-Q., Zhu Z. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Minghui 86.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY342319 mRNA. Translation: AAQ24869.1. CM000127 Genomic DNA. Translation: EAY86157.1.
3D structure databases
ProteinModelPortal	A2X5P7.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os02g33110.1.
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	A2X5P7.
Phylogenomic databases
eggNOG	COG1621.
HOGENOM	HOG000181427.
Gene expression databases
ArrayExpress	A2X5P7.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV1_ORYSI

Accession

Primary (citable) accession number: A2X5P7
Secondary accession number(s): Q6EU76, Q6VEF4, Q8S922

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	April 3, 2013
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program