A2X2H7 (BGAL4_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 4
Short name=Lactase 4
EC=3.2.1.23

Gene names

ORF Names:

OsI_006270

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 729	694	Beta-galactosidase 4		PRO_0000294155
Sites
	Active site	194	1	Proton donor Potential
	Active site	263	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	A2X2H7 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 190E85CECA84E8CE	FASTA	729	80,095
	10 20 30 40 50 60 MAPAPTPAAA AGRRVAVLAA ALVAASLAAS VGVANAAVSY DRRSLVINGR RRILLSGSIH 70 80 90 100 110 120 YPRSTPEMWP GLIQKAKDGG LDVIQTYVFW NGHEPVQGQY YFSDRYDLVR FVKLVKQAGL 130 140 150 160 170 180 YVHLRIGPYV CAEWNFGGFP VWLKYVPGVS FRTDNGPFKA EMQKFVEKIV SMMKSEGLFE 190 200 210 220 230 240 WQGGPIIMSQ VENEFGPMES VGGSGAKPYA NWAAKMAVRT NTGVPWVMCK QDDAPDPVIN 250 260 270 280 290 300 TCNGFYCDYF SPNKNYKPSM WTEAWTGWFT SFGGGVPHRP VEDLAFAVAR FIQKGGSFVN 310 320 330 340 350 360 YYMYHGGTNF GRTAGGPFIA TSYDYDAPID EFGLLRQPKW GHLRDLHRAI KQAEPVLVSA 370 380 390 400 410 420 DPTIESIGSY EKAYVFKAKN GACAAFLSNY HMNTAVKVRF NGQQYNLPAW SISILPDCKT 430 440 450 460 470 480 AVFNTATVKE PTLMPKMNPV VRFAWQSYSE DTNSLSDSAF TKDGLVEQLS MTWDKSDYLW 490 500 510 520 530 540 YTTYVNIGTN DLRSGQSPQL TVYSAGHSMQ VFVNGKSYGS VYGGYDNPKL TYNGRVKMWQ 550 560 570 580 590 600 GSNKISILSS AVGLPNVGNH FENWNVGVLG PVTLSSLNGG TKDLSHQKWT YQVGLKGETL 610 620 630 640 650 660 GLHTVTGSSA VEWGGPGGYQ PLTWHKAFFN APAGNDPVAL DMGSMGKGQL WVNGHHVGRY 670 680 690 700 710 720 WSYKASGGCG GCSYAGTYHE DKCRSNCGDL SQRWYHVPRS WLKPGGNLLV VLEEYGGDLA GVSLATRTT

References

[1]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000127 Genomic DNA. Translation: EAY85037.1.
3D structure databases
ProteinModelPortal	A2X2H7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	EPGT00070000028830.
HOGENOM	HBG318019.
PhylomeDB	A2X2H7.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL4_ORYSI

Accession

Primary (citable) accession number: A2X2H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 20, 2007
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program