A2X2G3 (PP2A3_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP2A-3 catalytic subunit
EC=3.1.3.16

Gene names

Name:	PP2A3
ORF Names:	OsI_006256

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2A subfamily.
Sequence caution	The sequence CM000127 differs from that shown. Reason: Frameshift at positions 39 and 52.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological_process	regulation of auxin polar transport Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Serine/threonine-protein phosphatase PP2A-3 catalytic subunit		PRO_0000301657
Sites
	Active site	116	1	Proton donor By similarity
	Metal binding	55	1	Iron By similarity
	Metal binding	57	1	Iron By similarity
	Metal binding	83	1	Iron By similarity
	Metal binding	83	1	Manganese By similarity
	Metal binding	115	1	Manganese By similarity
	Metal binding	165	1	Manganese By similarity
	Metal binding	239	1	Manganese By similarity
Experimental info
	Sequence conflict	60	1	F → P in CM000127. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A2X2G3 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 97143563695D0DD6	FASTA	307	35,145
	10 20 30 40 50 60 MPSSHGDLDR QIAQLRECKH LAEGEVRALC EQAKAILMEE WNVQPVRCPV TVCGDIHGQF 70 80 90 100 110 120 YDLIELFRIG GEAPDTNYLF MGDYVDRGYY SVETVSLLVA LKVRYRDRIT ILRGNHESRQ 130 140 150 160 170 180 ITQVYGFYDE CLRKYGNANV WKYFTDLFDY LPLTALIENQ VFCLHGGLSP SLDTLDNIRA 190 200 210 220 230 240 LDRIQEVPHE GPMCDLLWSD PDDRCGWGIS PRGAGYTFGQ DIAQQFNHTN GLSLISRAHQ 250 260 270 280 290 300 LVMEGFNWCQ DKNVVTVFSA PNYCYRCGNM AAILEIGENM DQNFLQFDPA PRQIEPDTTR KTPDYFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of protein phosphatase 2A catalytic subunit genes from Oryza sativa." Yu R.M.K., Kong R.Y.C. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. IR36.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.
[3]	"A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies." Liu X., Lu T., Yu S., Li Y., Huang Y., Huang T., Zhang L., Zhu J., Zhao Q., Fan D., Mu J., Shangguan Y., Feng Q., Guan J., Ying K., Zhang Y., Lin Z., Sun Z. , Qian Q., Lu Y., Han B. Plant Mol. Biol. 65:403-415(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Guang-Lu-Ai No.4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF159061 Genomic DNA. Translation: AAD41126.1. CM000127 Genomic DNA. No translation available. CT832108 mRNA. No translation available.
UniGene	Os.10337.
3D structure databases
ProteinModelPortal	A2X2G3.
SMR	A2X2G3. Positions 7-307.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	A2X2G3.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172696.
Gene expression databases
ArrayExpress	A2X2G3.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2A3_ORYSI

Accession

Primary (citable) accession number: A2X2G3
Secondary accession number(s): Q6Z6L8, Q6Z6L9, Q9XGT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	April 3, 2013
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program