Reviewed, UniProtKB/Swiss-Prot A2WXB2 (F16P2_ORYSI)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase, cytosolic
      Short name=FBPase
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase

Gene names

ORF Names:

OsI_04558

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Fructose-1,6-bisphosphatase, cytosolic		PRO_0000295021
Regions
	Region	124 – 127	4	Substrate binding By similarity
Sites
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	100	1	Magnesium 1 By similarity
	Metal binding	100	1	Magnesium 2 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	121	1	Magnesium 3 By similarity
	Metal binding	123	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	124	1	Magnesium 3 By similarity
	Metal binding	283	1	Magnesium 3 By similarity
	Binding site	215	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity
	Binding site	267	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2WXB2-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: C87583E7132E8AF3	FASTA	339	37,035
	10 20 30 40 50 60 MDHEADAYRT DLMTITRYVL NEQSRNPEAR GDLTILLSHI VLGCKFVASA VNKAGLAKLI 70 80 90 100 110 120 GLAGETNVQG EEQKKLDVLS NEVFVKALVS SGRTCVLVSE EDEEATFVDP ALRGKYCVCF 130 140 150 160 170 180 DPLDGSSNID CGVSIGTIFG IYMIKDKENV TLEDVLQPGK NMVAAGYCMY GSSCTLVLST 190 200 210 220 230 240 GNGVNGFTLD PSLGEFILTH PDIKIPKKGK IYSVNEGNAK NWDEPTAKFV EKCKFPKDGS 250 260 270 280 290 300 SPKSLRYIGS MVADVHRTLL YGGVFLYPAD KKSPNGKLRV LYEVFPMSFL MEQAGGQSFT 310 320 330 GKERALDLVP TKIHERSPIF LGSFEDVEEI KGLYAAQAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.
[2]	"Expression and regulation of genes involved in carbohydrate metabolism in rice." Lee D.-S., Hur Y. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 126-293. Strain: cv. Milyang 23.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000126 Genomic DNA. Translation: EEC71858.1. AF378183 mRNA. Translation: AAK54854.1.
3D structure databases
HSSP	HSSP built from PDB template 1FJ9 based on UniProtKB P00636.
ModBase	Search...
Organism-specific databases
Gramene	O64421.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P2_ORYSI

Accession

Primary (citable) accession number: A2WXB2
Secondary accession number(s): B8A6X6 , O64421, Q5N9E2, Q94JN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	October 13, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)