A2WPU3 (AMYC1_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase isozyme C
EC=3.2.1.1

Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Alpha-amylase isozyme 1B

Gene names

Name:	AMYC
Synonyms:	AMY1B
ORF Names:	OsI_001836

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Important for breakdown of endosperm starch during germination.
Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor	Binds 3 calcium ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Sequence caution	The sequence CAA36485.1 differs from that shown. Reason: Frameshift at several positions. The sequence CAA36485.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Alpha-amylase isozyme C		PRO_0000291431
Regions
	Region	87 – 88	2	Substrate binding By similarity
	Region	182 – 187	6	Substrate binding By similarity
	Region	289 – 291	3	Substrate binding By similarity
Sites
	Metal binding	116	1	Calcium By similarity
	Metal binding	188	1	Calcium; via carbonyl oxygen By similarity
	Binding site	214	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity
	Binding site	302	1	Substrate By similarity
	Binding site	344	1	Substrate By similarity
	Site	303	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2WPU3 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: 63D5C04555DCE02B	FASTA	348	38,508
	10 20 30 40 50 60 MQVPIEHNGG EQTLLVPFRA HRPPRPLLQL GSRASPVSGF QLGVVEGEWR VVQPAYGQAR 70 80 90 100 110 120 WTTSPPPASP TSGSLRRPTL SASKATCWGG CTIWTRPIIE AFHGKGVQVI ADIVINHRTA 130 140 150 160 170 180 EHKDSRGIYC RLPPRLGPAH DLPRRPLRRR HRKPGHRRRT STTSTSASSG SSSAGSTGSR 190 200 210 220 230 240 WTSASTRGAS TSPRATPPTW QRSTSMPPSR ASPWPRYGRR WRTAGTASRT TTRTRTGRSW 250 260 270 280 290 300 STGSIVSAAP TAMPRRSTSP PRASSTSPWR AIELWRLRGE DGKAPGMIGW WPAKATTFVD 310 320 330 340 NHDTGNPCIF YDHFFDWGLK DEIERLVSIR NRQGIHPAWG RCCWLLPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a high-pI rice (Oryza sativa) -amylase gene." Kim J.-K., Wu R. Plant Mol. Biol. 18:399-402(1992) [PubMed: 1731997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. IR26. Tissue: Leaf.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52240 Genomic DNA. Translation: CAA36485.1. Sequence problems. CM000126 Genomic DNA. No translation available.
PIR	ALRZOC. S19142.
3D structure databases
ProteinModelPortal	A2WPU3.
SMR	A2WPU3. Positions 96-130, 273-336.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P27940.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMYC1_ORYSI

Accession

Primary (citable) accession number: A2WPU3
Secondary accession number(s): P27940, Q5ZBK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program