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A2WKT4 (H2B5_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone H2B.5
Gene names
Name:H2B.5
ORF Names:OsI_000427
OrganismOryza sativa subsp. indica (Rice)
Taxonomic identifier39946 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length155 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit structure

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location

Nucleus By similarity. Chromosome By similarity.

Post-translational modification

Can be acetylated to form H2BK6ac and H2BK33ac By similarity.

Monoubiquitinated by BRE1 to form H2BK143ub1 and deubiquitinated by UBP26. Required for heterochromatic histone H3 di- and trimethylation at H3K4me. May give a specific tag for epigenetic transcriptional activation By similarity.

Sequence similarities

Belongs to the histone H2B family.

Caution

To ensure consistency between histone entries, we follow the 'Brno' nomenclature for histone modifications, with positions referring to those used in the literature for the 'closest' model organism. Due to slight variations in histone sequences between organisms and to the presence of initiator methionine in UniProtKB/Swiss-Prot sequences, the actual positions of modified amino acids in the sequence generally differ. In this entry the following conventions are used: H2BK6ac = acetylated Lys-7; H2BK33ac = acetylated Lys-37; H2BK143ub1 = monoubiquitinated Lys-151.

Ontologies

Keywords
   Cellular componentChromosome
Nucleosome core
Nucleus
   LigandDNA-binding
   PTMAcetylation
Isopeptide bond
Ubl conjugation
Gene Ontology (GO)
   Biological_processnucleosome assembly

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleosome

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 155154Histone H2B.5
PRO_0000294184

Amino acid modifications

Modified residue71N6-acetyllysine By similarity
Modified residue371N6-acetyllysine By similarity
Cross-link151Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
A2WKT4 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 8C9B2528C97AF413

FASTA15516,758
        10         20         30         40         50         60 
MAPKAEKKPA AKKPAEEEPA AEKAEKAPAG KKPKAEKRLP AGKGEKGSGE GKKDRAGRKK 

        70         80         90        100        110        120 
AKKSVETYKI YIFKVLKQVH PDIGISSKAM SIMNSFINDI FEKLAAEAAK LARYNKKPTI 

       130        140        150 
TSREIQTSVR LVLPGELAKH AVSEGTKAVT KFTSA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000126 Genomic DNA. Translation: EAY72580.1.

3D structure databases

ProteinModelPortalA2WKT4.
SMRA2WKT4. Positions 66-155.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39946.BGIOSIBCE000424.

Proteomic databases

PRIDEA2WKT4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneA2WKT4.

Phylogenomic databases

eggNOGCOG0173.
HOGENOMHOG000231213.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERPTHR23428. PTHR23428. 1 hit.
PfamPF00125. Histone. 1 hit.
[Graphical view]
PRINTSPR00621. HISTONEH2B.
SMARTSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMSSF47113. SSF47113. 1 hit.
PROSITEPS00357. HISTONE_H2B. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameH2B5_ORYSI
AccessionPrimary (citable) accession number: A2WKT4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 20, 2007
Last modified: December 11, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families