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Protein

Palmitoyltransferase app

Gene

app

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Palmitoylates Dlish which is required for the apical cell cortex localization, total cellular level and full activity of dachs.2 Publications

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei177S-palmitoyl cysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • protein-cysteine S-palmitoyltransferase activity Source: FlyBase

GO - Biological processi

  • establishment of body hair or bristle planar orientation Source: FlyBase
  • establishment of imaginal disc-derived wing hair orientation Source: FlyBase
  • pattern specification process Source: FlyBase
  • protein palmitoylation Source: FlyBase

Keywordsi

Molecular functionAcyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase app1 Publication (EC:2.3.1.225UniRule annotation1 Publication)
Alternative name(s):
Protein approximated1 Publication
Gene namesi
Name:app1 PublicationImported
ORF Names:CG42318Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0260941. app.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Transmembranei191 – 211HelicalSequence analysisAdd BLAST21
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • apical cortex Source: FlyBase
  • apical plasma membrane Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: FlyBase
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Semi-lethal in homozygotes and heterozygotes with adult escapers showing abdominal planar cell polarity defects and also extensive wing planar cell polarity defects, both proximally and in a distal region between the third and fourth longitudinal veins. Greatly reduced accumulation of dachs at the apical cell cortex.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004388521 – 693Palmitoyltransferase appAdd BLAST693

Keywords - PTMi

Lipoprotein, Palmitate

Expressioni

Tissue specificityi

Detected in embryo, imaginal disks and pupal wings (at protein level) (PubMed:18804377). Enriched in the somatic musculature and gut throughout embryonic development (PubMed:18719403).1 Publication

Gene expression databases

BgeeiFBgn0260941.
ExpressionAtlasiA2VEY9. differential.

Interactioni

Subunit structurei

Interacts with Dlish (via N-terminus including SH3 domain 1); this leads to palmitoylation of Dlish by app. Interacts with ft; this is likely to interfere with the interaction between app and Dlish, reducing the ability of app to palmitoylate Dlish and tether it to the apical cell cortex.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini147 – 197DHHCPROSITE-ProRule annotationAdd BLAST51

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi349 – 402Gly-richPROSITE-ProRule annotationAdd BLAST54

Domaini

The DHHC domain is required for palmitoyltransferase activity.UniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1311. Eukaryota.
COG5273. LUCA.
GeneTreeiENSGT00550000074293.
KOiK16675.
OrthoDBiEOG091G0BOW.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform LImported (identifier: A2VEY9-1) [UniParc]FASTAAdd to basket
Also known as: MImported, RImported, TImported

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLLCCCCCS NMAPNQRVTR KWELFAGRNK FYCDGLLMSA PHTGVFYLTC
60 70 80 90 100
ILITGTSALF FAFDCPFLAD SINPAIPIVG AVLYFFTMSS LLRTTFTDPG
110 120 130 140 150
VIPRASNDEA AYIEKQIEVP NSLNSPTYRP PPRTKEVLVK GQTVKLKYCF
160 170 180 190 200
TCKIFRPPRA SHCSLCDNCV DRFDHHCPWV GNCVGKRNYR FFYLFLVSLA
210 220 230 240 250
FLAVFIFSCS VTHLVLLMKK EHEVFNVIKA APFTVIVVFI CFFSIWSVIG
260 270 280 290 300
LAGFHTYLTT SDQTTNEDLK GSFSSKGGPR TQNPYSRGNI CLNCCHILCG
310 320 330 340 350
PMTPSLIDRR GIATDEFIQQ MQHQSSPRHA LSDVLSASHM VTTSQPMMGG
360 370 380 390 400
LGGGGIGGAG GGISIGGAEL KPRFYDESNP SSSTLEGNGG AINGHGNGHG
410 420 430 440 450
NGFDHPPPSY DLVQNGNSNS LAQLVDNEIP LVQMDIPAYT HQTATQARQY
460 470 480 490 500
RHRHHKQRQI CNGGTVSYEN QLANASSEDE LDDPDVVVVG SPEVVAAVAA
510 520 530 540 550
IASNKAREMR NRSGSYSNLF DADFEAALVN SSLADNHVRG EGASSSGKPS
560 570 580 590 600
AGAALLAAAI SGKTENMYSN VLPVDNDTDP ADSTLHVYSN IIDERKQQEQ
610 620 630 640 650
ANNVLSSTLL CDDLDLDDPV SASCHVKRKS LGDGAEQVKS AERLRMLHDN
660 670 680 690
TMIDTALDLD SLDGSSMGNN SQSCLVKSGK PNATSVTTNV AIV
Length:693
Mass (Da):75,008
Last modified:March 20, 2007 - v1
Checksum:i9D9CA4161D0ABF0E
GO
Isoform SImported (identifier: A2VEY9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-217: Missing.

Show »
Length:476
Mass (Da):50,519
Checksum:i718FAD406B75F18C
GO
Isoform HImported (identifier: A2VEY9-3) [UniParc]FASTAAdd to basket
Also known as: IImported

The sequence of this isoform differs from the canonical sequence as follows:
     378-398: SNPSSSTLEGNGGAINGHGNG → VSGQSSGHIESIQEHPFTTEI
     399-693: Missing.

Show »
Length:398
Mass (Da):43,895
Checksum:i5A9DB89EEBF92980
GO
Isoform OImported (identifier: A2VEY9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     378-382: SNPSS → VIPIV
     383-693: Missing.

Show »
Length:382
Mass (Da):42,151
Checksum:iDC41165B6E730A10
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0587471 – 217Missing in isoform S. CuratedAdd BLAST217
Alternative sequenceiVSP_058748378 – 398SNPSS…GHGNG → VSGQSSGHIESIQEHPFTTE I in isoform H. CuratedAdd BLAST21
Alternative sequenceiVSP_058749378 – 382SNPSS → VIPIV in isoform O. Curated5
Alternative sequenceiVSP_058750383 – 693Missing in isoform O. CuratedAdd BLAST311
Alternative sequenceiVSP_058751399 – 693Missing in isoform H. CuratedAdd BLAST295

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: ACL83291.1.
AE014296 Genomic DNA. Translation: ACL83292.1.
AE014296 Genomic DNA. Translation: ACL83293.2.
AE014296 Genomic DNA. Translation: ACL83294.2.
AE014296 Genomic DNA. Translation: AFH04402.2.
AE014296 Genomic DNA. Translation: AFH04403.1.
AE014296 Genomic DNA. Translation: AFH04404.2.
AE014296 Genomic DNA. Translation: AHN58068.1.
BT030308 mRNA. Translation: ABN49447.1.
RefSeqiNP_001137936.1. NM_001144464.2. [A2VEY9-1]
NP_001137937.1. NM_001144465.2. [A2VEY9-1]
NP_001137938.2. NM_001144466.3. [A2VEY9-3]
NP_001137939.2. NM_001144467.3. [A2VEY9-3]
NP_001246731.2. NM_001259802.2. [A2VEY9-1]
NP_001246732.1. NM_001259803.2. [A2VEY9-4]
NP_001246733.2. NM_001259804.2. [A2VEY9-2]
NP_001287043.1. NM_001300114.1. [A2VEY9-1]
UniGeneiDm.5785.

Genome annotation databases

EnsemblMetazoaiFBtr0301053; FBpp0290275; FBgn0260941. [A2VEY9-3]
FBtr0301056; FBpp0290278; FBgn0260941. [A2VEY9-1]
FBtr0301057; FBpp0290279; FBgn0260941. [A2VEY9-1]
FBtr0334102; FBpp0306224; FBgn0260941. [A2VEY9-1]
FBtr0334103; FBpp0306225; FBgn0260941. [A2VEY9-2]
FBtr0339925; FBpp0308957; FBgn0260941. [A2VEY9-1]
GeneIDi39399.
KEGGidme:Dmel_CG42318.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAPP_DROME
AccessioniPrimary (citable) accession number: A2VEY9
Secondary accession number(s): B7Z0A6, M9NDQ1, M9NF95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2017
Last sequence update: March 20, 2007
Last modified: June 7, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families