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A2VE78 (FBXL5_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
F-box/LRR-repeat protein 5
Alternative name(s):
F-box and leucine-rich repeat protein 5
Gene names
Name:FBXL5
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued By similarity.

Subcellular location

Cytoplasmperinuclear region By similarity.

Domain

The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded By similarity.

Post-translational modification

Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor By similarity.

Sequence similarities

Contains 1 F-box domain.

Contains 7 LRR (leucine-rich) repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691F-box/LRR-repeat protein 5
PRO_0000390464

Regions

Domain202 – 24847F-box
Repeat340 – 36425LRR 1
Repeat365 – 39228LRR 2
Repeat393 – 41826LRR 3
Repeat479 – 50830LRR 4
Repeat576 – 60732LRR 5
Repeat608 – 63528LRR 6
Repeat636 – 66126LRR 7
Region1 – 159159Hemerythrin-like By similarity

Sites

Metal binding151Iron 1 By similarity
Metal binding571Iron 1 By similarity
Metal binding581Iron 1 By similarity
Metal binding611Iron 1 By similarity
Metal binding611Iron 2 By similarity
Metal binding801Iron 2 By similarity
Metal binding1261Iron 2 By similarity
Metal binding1301Iron 1 By similarity
Metal binding1301Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A2VE78 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 15A5BA66BDE12331

FASTA69178,412
        10         20         30         40         50         60 
MAPFPEEVDV FTAPHWRMKQ LVGLYCDKLS KTNFSNNNDF RALLQSLYAT FKEFKMHEQI 

        70         80         90        100        110        120 
ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK NEYEQLNYAK QLKERLEAFT 

       130        140        150        160        170        180 
KDFLPHMKEE EEVFQPMLME YFTYEELKDI KKKVIAQHCS QKDTAELLRG LSLWNQAEER 

       190        200        210        220        230        240 
QKFFKYSVDE KSDKEAEVSE QSTGITHLPP EVMVSIFSYL NPQELCRCSQ VSTKWSQLAK 

       250        260        270        280        290        300 
TGSLWKHLYP VHWARGDWYS GPAAELDTEP DEEWVKSRRD ESRAFQEWDE DADIDESEES 

       310        320        330        340        350        360 
GEESIAISIA QMEKRLLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ ILELCPNLEH 

       370        380        390        400        410        420 
LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDVA LEKISRALGI LTTHESGLLK 

       430        440        450        460        470        480 
TSTSKVTSTT WKNKDITMQS FKQSACLHDV TNKDIGEEVD NEHPWTKPIS SDDFTSPYVW 

       490        500        510        520        530        540 
MLDAEDLADI EDAVEWRHRN VESLCVMETA SNFSCPSSAC YSKDIVGLRT SVCWQQHCAS 

       550        560        570        580        590        600 
PAFAYCGHSY CCTGTALRTM SALPESSALC RKAPRTRLLR EKDLIYSGSE KSDQETGRVL 

       610        620        630        640        650        660 
LFLSLSGCYQ ITDHGLRVLT LGGGLPYLEH LNLSGCLTVT GAGLQDLVSA CPSLNDEYFY 

       670        680        690 
YCDNINGPHA DTASGCQNLQ CGFRACCRSG E 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal lung.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC133613 mRNA. Translation: AAI33614.1.
RefSeqNP_001075909.1. NM_001082440.1.
UniGeneBt.17790.

3D structure databases

ProteinModelPortalA2VE78.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000028266.

Proteomic databases

PRIDEA2VE78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID519695.
KEGGbta:519695.

Organism-specific databases

CTD26234.

Phylogenomic databases

eggNOGNOG29620.
HOGENOMHOG000006899.
HOVERGENHBG051589.
InParanoidA2VE78.
KOK10271.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

InterProIPR001810. F-box_dom.
IPR012312. Haemerythrin/HHE_cat-bd_motif.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamPF01814. Hemerythrin. 1 hit.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 1 hit.
[Graphical view]
SUPFAMSSF81383. SSF81383. 1 hit.
PROSITEPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20872928.

Entry information

Entry nameFBXL5_BOVIN
AccessionPrimary (citable) accession number: A2VE78
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 20, 2007
Last modified: January 22, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways