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Protein

SUMO-activating enzyme subunit 1

Gene

SAE1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).By similarity

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-BTA-3065676. SUMO is conjugated to E1 (UBA2:SAE1).
R-BTA-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-activating enzyme subunit 1
Alternative name(s):
Ubiquitin-like 1-activating enzyme E1A
Cleaved into the following chain:
Gene namesi
Name:SAE1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004232891 – 346SUMO-activating enzyme subunit 1Add BLAST346
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00003281372 – 346SUMO-activating enzyme subunit 1, N-terminally processedAdd BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylvaline; in SUMO-activating enzyme subunit 1, N-terminally processedBy similarity1
Modified residuei12PhosphoserineBy similarity1
Modified residuei198N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiA2VE14.
PeptideAtlasiA2VE14.
PRIDEiA2VE14.

Expressioni

Gene expression databases

BgeeiENSBTAG00000002676.

Interactioni

Subunit structurei

Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000003467.

Structurei

3D structure databases

ProteinModelPortaliA2VE14.
SMRiA2VE14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Phylogenomic databases

eggNOGiKOG2014. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00550000075007.
HOGENOMiHOG000172217.
HOVERGENiHBG080782.
InParanoidiA2VE14.
KOiK10684.
OMAiSYCFSEM.
OrthoDBiEOG091G0DZ1.
TreeFamiTF315037.

Family and domain databases

InterProiView protein in InterPro
IPR000594. ThiF_NAD_FAD-bd.
IPR035985. Ubiquitin-activating_enz.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
PfamiView protein in Pfam
PF00899. ThiF. 1 hit.
PRINTSiPR01849. UBIQUITINACT.
SUPFAMiSSF69572. SSF69572. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2VE14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEKEEAGGG ISEEEAAQYD RQIRLWGLEA QKRLRASQVL LVGMKGLGAE
60 70 80 90 100
IAKNLILAGV KGLTMLDHEQ VSPEDPGAQF LIRTGSVGRN RAEASLERAQ
110 120 130 140 150
NLNPMVDVKV DTENIEKKPE SFFTQFDAVC LTCCSRDVIV KVDQICHKNS
160 170 180 190 200
IKFFTGDVFG YHGYTFANLG EHEFVEEKTK VAKVSQGVED GPDTKRAKLD
210 220 230 240 250
SSETTMVKKK VVFCSVKEAL EVDWSSDKAK AALKRTTPDY FLLQVLLKFR
260 270 280 290 300
TDKGRDPSSD TFGEDSELLL QIRNDVLDAL GVNPDLLPED FVRYCFSEMA
310 320 330 340
PVCAVVGGIL AQEIVKALSQ RDPPHNNFFF FDGMKGNGIV ECLGPN
Length:346
Mass (Da):38,306
Last modified:March 20, 2007 - v1
Checksum:i04CE3BE656FA8EC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133519 mRNA. Translation: AAI33520.1.
RefSeqiNP_001075180.1. NM_001081711.1.
UniGeneiBt.10575.

Genome annotation databases

EnsembliENSBTAT00000003467; ENSBTAP00000003467; ENSBTAG00000002676.
GeneIDi505512.
KEGGibta:505512.

Similar proteinsi

Entry informationi

Entry nameiSAE1_BOVIN
AccessioniPrimary (citable) accession number: A2VE14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 20, 2007
Last modified: October 25, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families