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Protein

E3 ubiquitin-protein ligase BRE1A

Gene

RNF20

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of PA2G4 leading to its proteasome-mediated degradation.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri922 – 961RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1A (EC:2.3.2.27By similarity)
Short name:
BRE1-A
Alternative name(s):
RING finger protein 20
RING-type E3 ubiquitin transferase BRE1ACurated
Gene namesi
Name:RNF20
Synonyms:BRE1A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003277161 – 975E3 ubiquitin-protein ligase BRE1AAdd BLAST975

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21N6-acetyllysineBy similarity1
Modified residuei41PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei138PhosphoserineBy similarity1
Modified residuei348N6-acetyllysineBy similarity1
Modified residuei510N6-acetyllysineBy similarity1
Modified residuei522PhosphoserineBy similarity1
Modified residuei562PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiA2VDP1.
PRIDEiA2VDP1.

Expressioni

Gene expression databases

BgeeiENSBTAG00000009516.

Interactioni

Subunit structurei

Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Interacts with PA2G4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012521.

Structurei

3D structure databases

ProteinModelPortaliA2VDP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili43 – 90Sequence analysisAdd BLAST48
Coiled coili168 – 375Sequence analysisAdd BLAST208
Coiled coili429 – 898Sequence analysisAdd BLAST470

Sequence similaritiesi

Belongs to the BRE1 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri922 – 961RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
GeneTreeiENSGT00390000002866.
HOGENOMiHOG000231526.
HOVERGENiHBG080312.
InParanoidiA2VDP1.
KOiK10696.
OMAiNKELAGN.
OrthoDBiEOG091G01SA.
TreeFamiTF323183.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2VDP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGIGSKRAA GEPGTSVPPE KKTAVEDSGT TVETIKLGGV SSTEELDIRT
60 70 80 90 100
LQTKNRKLAE MLDQRQAIED ELREHIEKLE RRQATDDASL LIVNRYWSQF
110 120 130 140 150
DENIRIILKR YDLEQGLGDL LTERKALVVP EPEPDSDSNQ ERKDDRERGE
160 170 180 190 200
GQEPAFSFLA TLASSSSEEM ESQLQERVES SRRAVSQIVT VYDKLQEKVE
210 220 230 240 250
LLSRKLNSGD SLMVEEAVQE LNSFLAQENT RLQELTDLLQ EKHCTMSQEF
260 270 280 290 300
SKLQSKVETA ESRVSVLESM IDDLQWDIDK IRKREQRLNR HLAEVLERVN
310 320 330 340 350
SKGYKVYGAG SSLYGGTITI NARKFEEMNA ELEENKELAQ NRHCELEKLR
360 370 380 390 400
QDFEEVTSQN EKLKVELRSA VEEVVKETPE YRCMQSQFSV LYNESLQLKA
410 420 430 440 450
HLDEARTLLH GTRGTHQRQV ELIERDEVSL HKKLRTEVIQ LEDTLAQVRK
460 470 480 490 500
EYEMLRIEFE QTLAANEQAG PINREMRHLI SSLQNHNHQL KGEVLRYKRK
510 520 530 540 550
LREAQSDLNK TRLRSGSALL QSQSSTEDPK DEPAELKQDS EDLATQSAAS
560 570 580 590 600
KASQEEVNEI KSKRDEEERE RERREKERER EREREKEKER EREKQKLKES
610 620 630 640 650
EKERESAKDK EKGKHDDGRK KEAEIIKQLK IELKKAQESQ KEMKLLLDMY
660 670 680 690 700
RSAPKEQRDK VQLMAAEKKS KAELEDLRQR LKDLEDKEKK ENKKMADEDA
710 720 730 740 750
LRKIRAVEEQ IEYLQKKLAM AKQEEEALLS EMDVTGQAFE DMQEQNIRLM
760 770 780 790 800
QQLREKDDAN FKLMSERIKS NQIHKLLKEE KEELADQVLT LKTQVDAQLQ
810 820 830 840 850
VVRKLEEKEH LLQSNIGTGE KELGLRTQAL EMNKRKAMEA AQLADDLKAQ
860 870 880 890 900
LEMAQKKLHD FQDEIVENSV TKEKDMFNFK RAQEDISRLR RKLETTKKPD
910 920 930 940 950
NVPKCDEILM EEIKDYKARL TCPCCNMRKK DAVLTKCFHV FCFECVKTRY
960 970
DTRQRKCPKC NAAFGANDFH RIYIG
Length:975
Mass (Da):113,658
Last modified:March 20, 2007 - v1
Checksum:iC297567458FC0C0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133334 mRNA. Translation: AAI33335.1.
RefSeqiNP_001075056.1. NM_001081587.1.
XP_010806508.1. XM_010808206.2.
XP_010806510.1. XM_010808208.2.
UniGeneiBt.10397.

Genome annotation databases

EnsembliENSBTAT00000012521; ENSBTAP00000012521; ENSBTAG00000009516.
GeneIDi513326.
KEGGibta:513326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133334 mRNA. Translation: AAI33335.1.
RefSeqiNP_001075056.1. NM_001081587.1.
XP_010806508.1. XM_010808206.2.
XP_010806510.1. XM_010808208.2.
UniGeneiBt.10397.

3D structure databases

ProteinModelPortaliA2VDP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012521.

Proteomic databases

PaxDbiA2VDP1.
PRIDEiA2VDP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012521; ENSBTAP00000012521; ENSBTAG00000009516.
GeneIDi513326.
KEGGibta:513326.

Organism-specific databases

CTDi56254.

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
GeneTreeiENSGT00390000002866.
HOGENOMiHOG000231526.
HOVERGENiHBG080312.
InParanoidiA2VDP1.
KOiK10696.
OMAiNKELAGN.
OrthoDBiEOG091G01SA.
TreeFamiTF323183.

Enzyme and pathway databases

UniPathwayiUPA00143.

Gene expression databases

BgeeiENSBTAG00000009516.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRE1A_BOVIN
AccessioniPrimary (citable) accession number: A2VDP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 20, 2007
Last modified: November 30, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.