A2VDN5 (SPAST_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	SPAST
Synonyms:	SPG4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. Binding to ATP stabilizes the homohexameric form. Binds to microtubules at least in part via the alpha-tubulin and beta-tubulin tails. The hexamer may adopt a ring conformation through which microtubules pass prior to being severed. Does not interact strongly with tubulin heterodimers. Interacts (via MIT domain) with CHMP1B; the interaction is direct. Interacts with ATL1, RTN1, SSNA1 and ZFYVE27 By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Endoplasmic reticulum By similarity. Endosome By similarity. Nucleus By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Endoplasmic reticulum Endosome Membrane Microtubule Nucleus
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis, completion of separation Inferred from sequence or structural similarity. Source: UniProtKB microtubule bundle formation Inferred from sequence or structural similarity. Source: UniProtKB microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB nervous system development Inferred from electronic annotation. Source: UniProtKB-KW protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Spastin		PRO_0000367133
Regions
	Transmembrane	57 – 77	21	Helical; Potential
	Domain	117 – 193	77	MIT
	Nucleotide binding	380 – 387	8	ATP Potential
	Region	1 – 298	298	Required for interaction with RTN1 By similarity
	Region	1 – 192	192	Required for midbody localization By similarity
	Region	1 – 80	80	Required for interaction with ATL1 By similarity
	Region	1 – 50	50	Required for nuclear localization By similarity
	Region	50 – 87	38	Required for interaction with SSNA1 and microtubules By similarity
	Region	110 – 194	85	Sufficient for interaction with CHMP1B By similarity
	Region	111 – 198	88	Required for interaction with microtubules By similarity
	Region	225 – 326	102	Sufficient for interaction with microtubules By similarity
	Region	226 – 614	389	Sufficient for microtubule severing By similarity
	Region	268 – 326	59	Required for interaction with microtubules and microtubule severing By similarity
	Motif	4 – 11	8	Nuclear localization signal By similarity
	Motif	59 – 67	9	Nuclear export signal By similarity
	Motif	307 – 310	4	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	266	1	Phosphoserine By similarity
	Modified residue	301	1	Phosphothreonine By similarity
	Modified residue	304	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2VDN5 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: B284B6EBF04D358F	FASTA	614	67,225
	10 20 30 40 50 60 MNSPGGRGKK KGSGGPSSPV PPRPPPPCQA RSRPAPKPAP PPQSPHKRNL YYFSYPLFLG 70 80 90 100 110 120 FALLRLVAFH LGLLFVWLCQ RFSRALMAAK RSSGAAPASA SPPAPVPGGE AERVRAFHKQ 130 140 150 160 170 180 AFEYISVALR IDEDEKVGQK DQAVEWYKKG IEELEKGIAV VVTGQGEQCE RARRLQAKMM 190 200 210 220 230 240 TNLVMAKDRL QLLEKLQPSL QFSKSQTDVY NDSTNLTCRN GHLQSESGAV PKRKDPLTHA 250 260 270 280 290 300 SNSLPRSKTV MKTGPTGLSG HHRAPSCSGL SMVSGVRQGP GSAAATHKST PKTNRTNKPS 310 320 330 340 350 360 TPTTAARKKK DLKNFRNVDS NLANLIMNEI VDNGTAVKFD DIAGQELAKQ ALQEIVILPS 370 380 390 400 410 420 LRPELFTGLR APARGLLLFG PPGNGKTMLA KAVAAESNAT FFNISAASLT SKYVGEGEKL 430 440 450 460 470 480 VRALFAVARE LQPSIIFIDE VDSLLCERRE GEHDASRRLK TEFLIEFDGV QSAGDDRVLV 490 500 510 520 530 540 MGATNRPQEL DEAVLRRFTK RVYVSLPNEE TRLLLLKNLL CKQGSPLTQK ELAQLARMTN 550 560 570 580 590 600 GYSGSDLTAL AKDAALGPIR ELKPEQVKNM SASEMRNIRL SDFTESLKKI KRSVSPQTLE 610 AYIRWNKDFG DTTV

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC133327 mRNA. Translation: AAI33328.1.
IPI	IPI00840116.
RefSeq	NP_001075060.1. NM_001081591.1.
UniGene	Bt.46123.
3D structure databases
ProteinModelPortal	A2VDN5.
SMR	A2VDN5. Positions 322-606.
ModBase	Search...
Protein-protein interaction databases
STRING	A2VDN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000046919; ENSBTAP00000044166; ENSBTAG00000021694.
GeneID	521442.
KEGG	bta:521442.
Organism-specific databases
CTD	6683.
Phylogenomic databases
eggNOG	maNOG06017.
GeneTree	ENSGT00570000078874.
HOVERGEN	HBG108502.
InParanoid	A2VDN5.
OrthoDB	EOG4NZTTF.
PhylomeDB	A2VDN5.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
KO	K13254.
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_BOVIN

Accession

Primary (citable) accession number: A2VDN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 20, 2007
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program