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A2VDM8

- BAP1_BOVIN

UniProt

A2VDM8 - BAP1_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase BAP1

Gene

BAP1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911NucleophileBy similarity
    Active sitei169 – 1691Proton donorBy similarity
    Sitei184 – 1841Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
    2. monoubiquitinated protein deubiquitination Source: UniProtKB
    3. protein deubiquitination Source: UniProtKB
    4. protein K48-linked deubiquitination Source: UniProtKB
    5. regulation of cell cycle Source: UniProtKB
    6. regulation of cell growth Source: UniProtKB
    7. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Chromatin regulator, Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase BAP1 (EC:3.4.19.12)
    Alternative name(s):
    BRCA1-associated protein 1
    Gene namesi
    Name:BAP1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. PR-DUB complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 711711Ubiquitin carboxyl-terminal hydrolase BAP1PRO_0000395816Add
    BLAST

    Post-translational modificationi

    Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) by UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention By similarity.By similarity

    Keywords - PTMi

    Ubl conjugation

    Interactioni

    Subunit structurei

    Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA2VDM8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni578 – 703126Interaction with BRCA1By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili612 – 64332Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi345 – 3484HBM-like motifBy similarity
    Motifi699 – 7046Nuclear localization signalBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family. BAP1 subfamily.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    HOVERGENiHBG054042.
    KOiK08588.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.

    Sequencei

    Sequence statusi: Complete.

    A2VDM8-1 [UniParc]FASTAAdd to Basket

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    MNKGWLELES DPGLFTLLVE DFGVKGVQVE EIYDLQSKCQ GPVYGFIFLF    50
    KWIEERRSRR KVSTLVDDTS VIDDDIVNNM FFAHQLIPNS CATHALLSVL 100
    LNCSNVDLGP TLSRMKDFTK GFSPESKGYA IGNAPELAKA HNSHARPEPR 150
    HLPEKQNGLS AVRTMEAFHF VSYVPITGRL FELDGLKVYP IDHGPWGEDE 200
    EWTDKARRVI MERIGLATAG IKYEARLHVL KVNRQTVLEA LQQLIRVTQP 250
    ELIQTHKSQE SQLPEESKPA SSKSPLALET SRAPVASEST HTDGVEEVAG 300
    SCPQAPTHSP PSKPKLVVKP PGSNINGVPP NPTPIVQRLP AFLDNHNYAK 350
    SPMQEEEDLA AGVGRSRVPV RPPQQYSDDE DDYEDEEEDD AQSTSSAIRY 400
    KRKGPGKPGP LSSSGDGQLS VLQPNTINVL AEKLKESQKD LSIPLSIKTS 450
    SGAGSPAVAV PTHSQPSPTP SNESTDTASE IGSAFNSPLR SPIRSANPTR 500
    PSSPVTSHIS KVLFGEDDSL LRVDCIRYNR AVRDLGPVIS TGLLHLAEDG 550
    VLSPLALTES GKGSSPSIRP SQGSQGSGSP EEKEVVEAVD SREKPGLVRP 600
    SESLNGEKYS PKELLALLKC VEAEIANYEA CLKEEVEKRK KFKIDDQRRT 650
    HNYDEFICTF ISMLAQEGML ANLVEQNISV RRRQGVSIGR LHKQRKPDRR 700
    KRSRPYKAKR Q 711
    Length:711
    Mass (Da):78,333
    Last modified:March 20, 2007 - v1
    Checksum:i31D9E5B4AF11C286
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC133317 mRNA. Translation: AAI33318.1.
    RefSeqiNP_001096019.1. NM_001102549.1.
    UniGeneiBt.20209.

    Genome annotation databases

    GeneIDi100124510.
    KEGGibta:100124510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC133317 mRNA. Translation: AAI33318.1 .
    RefSeqi NP_001096019.1. NM_001102549.1.
    UniGenei Bt.20209.

    3D structure databases

    ProteinModelPortali A2VDM8.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C12.004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100124510.
    KEGGi bta:100124510.

    Organism-specific databases

    CTDi 8314.

    Phylogenomic databases

    HOVERGENi HBG054042.
    KOi K08588.

    Miscellaneous databases

    NextBioi 20788729.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Basal ganglia.

    Entry informationi

    Entry nameiBAP1_BOVIN
    AccessioniPrimary (citable) accession number: A2VDM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3