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Protein

Ubiquitin carboxyl-terminal hydrolase BAP1

Gene

BAP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91NucleophileBy similarity1
Active sitei169Proton donorBy similarity1
Sitei184Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase BAP1 (EC:3.4.19.12)
Alternative name(s):
BRCA1-associated protein 1
Gene namesi
Name:BAP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003958161 – 711Ubiquitin carboxyl-terminal hydrolase BAP1Add BLAST711

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei274PhosphoserineBy similarity1
Modified residuei351PhosphoserineBy similarity1
Modified residuei377PhosphoserineBy similarity1
Modified residuei503PhosphoserineBy similarity1
Modified residuei519PhosphoserineBy similarity1
Modified residuei567PhosphoserineBy similarity1
Modified residuei579PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) by UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiA2VDM8.

Interactioni

Subunit structurei

Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054730.

Structurei

3D structure databases

ProteinModelPortaliA2VDM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni578 – 703Interaction with BRCA1By similarityAdd BLAST126

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili612 – 643Sequence analysisAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi345 – 348HBM-like motifBy similarity4
Motifi699 – 704Nuclear localization signalBy similarity6

Sequence similaritiesi

Belongs to the peptidase C12 family. BAP1 subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2778. Eukaryota.
ENOG410XP0P. LUCA.
HOVERGENiHBG054042.
InParanoidiA2VDM8.
KOiK08588.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

A2VDM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKGWLELES DPGLFTLLVE DFGVKGVQVE EIYDLQSKCQ GPVYGFIFLF
60 70 80 90 100
KWIEERRSRR KVSTLVDDTS VIDDDIVNNM FFAHQLIPNS CATHALLSVL
110 120 130 140 150
LNCSNVDLGP TLSRMKDFTK GFSPESKGYA IGNAPELAKA HNSHARPEPR
160 170 180 190 200
HLPEKQNGLS AVRTMEAFHF VSYVPITGRL FELDGLKVYP IDHGPWGEDE
210 220 230 240 250
EWTDKARRVI MERIGLATAG IKYEARLHVL KVNRQTVLEA LQQLIRVTQP
260 270 280 290 300
ELIQTHKSQE SQLPEESKPA SSKSPLALET SRAPVASEST HTDGVEEVAG
310 320 330 340 350
SCPQAPTHSP PSKPKLVVKP PGSNINGVPP NPTPIVQRLP AFLDNHNYAK
360 370 380 390 400
SPMQEEEDLA AGVGRSRVPV RPPQQYSDDE DDYEDEEEDD AQSTSSAIRY
410 420 430 440 450
KRKGPGKPGP LSSSGDGQLS VLQPNTINVL AEKLKESQKD LSIPLSIKTS
460 470 480 490 500
SGAGSPAVAV PTHSQPSPTP SNESTDTASE IGSAFNSPLR SPIRSANPTR
510 520 530 540 550
PSSPVTSHIS KVLFGEDDSL LRVDCIRYNR AVRDLGPVIS TGLLHLAEDG
560 570 580 590 600
VLSPLALTES GKGSSPSIRP SQGSQGSGSP EEKEVVEAVD SREKPGLVRP
610 620 630 640 650
SESLNGEKYS PKELLALLKC VEAEIANYEA CLKEEVEKRK KFKIDDQRRT
660 670 680 690 700
HNYDEFICTF ISMLAQEGML ANLVEQNISV RRRQGVSIGR LHKQRKPDRR
710
KRSRPYKAKR Q
Length:711
Mass (Da):78,333
Last modified:March 20, 2007 - v1
Checksum:i31D9E5B4AF11C286
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133317 mRNA. Translation: AAI33318.1.
RefSeqiNP_001096019.1. NM_001102549.1.
UniGeneiBt.20209.

Genome annotation databases

GeneIDi100124510.
KEGGibta:100124510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133317 mRNA. Translation: AAI33318.1.
RefSeqiNP_001096019.1. NM_001102549.1.
UniGeneiBt.20209.

3D structure databases

ProteinModelPortaliA2VDM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054730.

Protein family/group databases

MEROPSiC12.004.

Proteomic databases

PaxDbiA2VDM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100124510.
KEGGibta:100124510.

Organism-specific databases

CTDi8314.

Phylogenomic databases

eggNOGiKOG2778. Eukaryota.
ENOG410XP0P. LUCA.
HOVERGENiHBG054042.
InParanoidiA2VDM8.
KOiK08588.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
ProtoNetiSearch...

Entry informationi

Entry nameiBAP1_BOVIN
AccessioniPrimary (citable) accession number: A2VDM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 20, 2007
Last modified: July 6, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.