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Protein

ATP-dependent RNA helicase DDX1

Gene

ddx1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Acts as a positive regulator of transcription. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Binds DNA and RNA. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit rtcb (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Exonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, Transcription, Transcription regulation, tRNA processing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX1 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 1
Gene namesi
Name:ddx1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-969102. ddx1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 740740ATP-dependent RNA helicase DDX1PRO_0000312360Add
BLAST

Proteomic databases

PRIDEiA2VD92.

Interactioni

Protein-protein interaction databases

IntActiA2VD92. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliA2VD92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 428427Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini70 – 247178B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST
Domaini493 – 681189Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi370 – 3734DEAD box

Domaini

The helicase domain is involved in the stimulation of RELA transcriptional activity.By similarity

Sequence similaritiesi

Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG005462.
KOiK13177.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF52540. SSF52540. 3 hits.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2VD92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFSEMGVM PEIAQAVEEM DWLLPTDIQA ESIPLILGGG DVLMAAETGS
60 70 80 90 100
GKTGAFSIPV IQIVYETLKD QQEGKKGKAS VKTGSTVLNK WQMNPYDRGS
110 120 130 140 150
AFAIGSDGLC CQSREIKEWH GCRSTRGVNK GKYYYEVSCH DQGLCRVGWS
160 170 180 190 200
TLSASLDLGT DKFGFGFGGT GKKSHNKQFD NYGEEFTMHD TIGCYLDIDN
210 220 230 240 250
SIVKFSKNGK DLGLAFQIPS HMKNQAFFTS CVLKNAELKF NFGEEDFKFP
260 270 280 290 300
PKDGFVALSK APDGHVVKSQ NTGSAQVSQT KSLPNAPKAL IIEPSRELAE
310 320 330 340 350
QTLNNVKQFK KYVDNPKLRE LLIIGGVAAK EQLTILENGV DIVVGTPGRI
360 370 380 390 400
DDLISTGKLS LSQVRFLVLD EADGLLSQGY SDFINRIYGQ IPQITSDGKR
410 420 430 440 450
LQVIVCSATL HSFDVKKLSE KIMHFPTWVD LKGEDSVPET VHHVVVPVNP
460 470 480 490 500
KKDKQWEKLA KNHIRTDGVH DKDNTRPGGN SAEVWSEAIK VLKGEYIVRA
510 520 530 540 550
IKEHKMDQAI IFCRTKLDCD NMEQYFIQQG GGPDKKGHQF SCVCLHSDRK
560 570 580 590 600
PPERKHNLER FKKCEVRFLI CTDVAARGID IRGVPYVINV TLPDEKQNYV
610 620 630 640 650
HRIGRVGRAE RMGLAISLVA SEKEKVWYHV CSSRGKGCYN TRLKEDGGCT
660 670 680 690 700
IWYNEMQLLS EIEEHLTCTI SQVEPDIKVP LDDFDGKVVY GQRRATGGGL
710 720 730 740
YKGHVDILAP TVQELASLEK EAQTSFLHLG YLSNQLFRSF
Length:740
Mass (Da):82,347
Last modified:March 20, 2007 - v1
Checksum:iE55339BC02B6407B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC129617 mRNA. Translation: AAI29618.1.
RefSeqiNP_001091352.1. NM_001097883.1.
UniGeneiXl.75260.

Genome annotation databases

GeneIDi100037192.
KEGGixla:100037192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC129617 mRNA. Translation: AAI29618.1.
RefSeqiNP_001091352.1. NM_001097883.1.
UniGeneiXl.75260.

3D structure databases

ProteinModelPortaliA2VD92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA2VD92. 1 interaction.

Proteomic databases

PRIDEiA2VD92.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100037192.
KEGGixla:100037192.

Organism-specific databases

CTDi1653.
XenbaseiXB-GENE-969102. ddx1.

Phylogenomic databases

HOVERGENiHBG005462.
KOiK13177.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF52540. SSF52540. 3 hits.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX1_XENLA
AccessioniPrimary (citable) accession number: A2VD92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: March 20, 2007
Last modified: July 6, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.