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Protein

Cyclic AMP-responsive element-binding protein 3-like protein 2

Gene

creb3l2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/mbtps1 and S2P/mbtps2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis. May protect neuroblastoma cells from ER stress-induced death. In vitro activates transcription of target genes via direct binding to the CRE site.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-responsive element-binding protein 3-like protein 2
Short name:
cAMP-responsive element-binding protein 3-like protein 2
Cleaved into the following chain:
Gene namesi
Name:creb3l2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-996214. creb3l2.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein By similarity

  • Note: ER membrane resident protein. Upon ER stress, translocated to the Golgi apparatus where it is cleaved. The cytosolic N-terminal fragment (processed cyclic AMP-responsive element-binding protein 3-like protein 1) is transported into the nucleus.By similarity
Processed cyclic AMP-responsive element-binding protein 3-like protein 2 :
  • Nucleus By similarity

  • Note: Upon ER stress, translocated into the nucleus.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 382382CytoplasmicSequence analysisAdd
BLAST
Transmembranei383 – 40321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini404 – 525122LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Cyclic AMP-responsive element-binding protein 3-like protein 2PRO_0000288072Add
BLAST
Chaini1 – ?Processed cyclic AMP-responsive element-binding protein 3-like protein 2PRO_0000296214

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence analysis
Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence analysis
Glycosylationi522 – 5221N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/mbtps1 and S2P/mbtps2).By similarity

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Binds DNA as a dimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA2VD01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini299 – 36264bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni301 – 33030Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni341 – 36222Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi433 – 4364S1P recognitionBy similarity

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG057480.
KOiK09048.

Family and domain databases

InterProiIPR029804. BBF2H7.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR22952:SF96. PTHR22952:SF96. 3 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2VD01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIMESGDPV IQWDRKLSEL SEAAESDSLY NNTPFSELLD DSALLDVLGQ
60 70 80 90 100
LMGDPFLTEK YEMMEVEMNP SSPSPMIKAE HSYSLCGDSR PQSPFTHASS
110 120 130 140 150
DDNFSDTDLT GDDWCLNGEL TATTPTTKIK VEIPLEETPG LTPSVTLATS
160 170 180 190 200
AVSASPEVGV SSQLPVPEQV KLLSPVALPQ IKLEPHEVDQ FLNLCPKEVA
210 220 230 240 250
PTEALQMPPT PPSSHGSDSE GGQSPTRSLP PSSPVQSQAG GKMAARSPSA
260 270 280 290 300
LSNSPLLTAP HKLQGSGPLM LTEEEKRTLV AEGYPIPTKL PLTKAEEKAL
310 320 330 340 350
KKIRRKIKNK ISAQESRRKK KEYMDSLEKR VENSSSENSE LRKKVEVLES
360 370 380 390 400
TNRTLLQQLQ RLQAMVTGKV TRSCKAAGTQ TGTCLMMVVL CFAVIFGSFT
410 420 430 440 450
QNLDMYSSSS KTIHEPSQYS APESYAASIV RSRKLLIFEE HQAVEELHSS
460 470 480 490 500
AVMLETQDTW EVQADTISKQ QAALLEELHL SQEKPFSLSN DSSSDMPVRH
510 520
RFTSEFGHND TTKVIELDRT VNTTS
Length:525
Mass (Da):57,625
Last modified:March 20, 2007 - v1
Checksum:i44644A533FA13D9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC129061 mRNA. Translation: AAI29062.1.
RefSeqiNP_001091333.1. NM_001097864.1.
UniGeneiXl.9410.

Genome annotation databases

GeneIDi100037168.
KEGGixla:100037168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC129061 mRNA. Translation: AAI29062.1.
RefSeqiNP_001091333.1. NM_001097864.1.
UniGeneiXl.9410.

3D structure databases

ProteinModelPortaliA2VD01.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100037168.
KEGGixla:100037168.

Organism-specific databases

CTDi64764.
XenbaseiXB-GENE-996214. creb3l2.

Phylogenomic databases

HOVERGENiHBG057480.
KOiK09048.

Family and domain databases

InterProiIPR029804. BBF2H7.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR22952:SF96. PTHR22952:SF96. 3 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCR3L2_XENLA
AccessioniPrimary (citable) accession number: A2VD01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 20, 2007
Last modified: December 9, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.