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A2VCW9 (AASS_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alpha-aminoadipic semialdehyde synthase, mitochondrial
Alternative name(s):
LKR/SDH

Including the following 2 domains:

  1. Lysine ketoglutarate reductase
    Short name=LKR
    Short name=LOR
    EC=1.5.1.8
  2. Saccharopine dehydrogenase
    Short name=SDH
    EC=1.5.1.9
Gene names
Name:Aass
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length926 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively By similarity. UniProtKB Q9UDR5

Catalytic activity

N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH. UniProtKB Q9UDR5

N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADH. UniProtKB Q9UDR5

Pathway

Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 1/6.

Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6.

Subunit structure

Homodimer By similarity. UniProtKB Q9UDR5

Subcellular location

Mitochondrion By similarity UniProtKB Q9UDR5.

Sequence similarities

In the N-terminal section; belongs to the AlaDH/PNT family.

In the C-terminal section; belongs to the saccharopine dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3232Mitochondrion By similarity UniProtKB Q9UDR5
Chain33 – 926894Alpha-aminoadipic semialdehyde synthase, mitochondrial UniProtKB Q9UDR5
PRO_0000315868

Regions

Region33 – 455423Lysine-ketoglutarate reductase By similarity UniProtKB Q9UDR5
Region477 – 926450Saccharopine dehydrogenase By similarity UniProtKB Q9UDR5

Sequences

Sequence LengthMass (Da)Tools
A2VCW9 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 4350DDB240B4075F

FASTA926102,908
        10         20         30         40         50         60 
MLRAQRLRLA RLRACVSRGL HHKPVMALRR EDVNAWERRA PLAPKHIKGI TKLGYKVLIQ 

        70         80         90        100        110        120 
PSNRRAIHDK EYVRAGGILQ EDITEACLIL GVKRPPEEKL MSKKTYAFFS HTIKAQEANM 

       130        140        150        160        170        180 
SLLDEVLKQE IRLIDYEKMV DHRGSRIVAF GHWAGVAGMI NILHGMGLRL LALGHHTPFM 

       190        200        210        220        230        240 
HLGMAHNYRN SSQAVQAVRD AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQEVFNELPC 

       250        260        270        280        290        300 
EYVEPHELKE VSKTGDLRKV YGTVLSRHHH LVRKTDGVYD PVEYEKYPER YISRFNADIA 

       310        320        330        340        350        360 
PYTTCLINGI YWEQNTPRLL TRQDAQSLLV PVKSSVVPVE GCPELPHKLV AICDISADTG 

       370        380        390        400        410        420 
GSIDFMTECT TIERPFCMYD ADQHIIHDSV EGSGILMCSI DNLPAQLPIE ATEYFGDMLY 

       430        440        450        460        470        480 
PYVEEMLLSD ASQPLESQNF SPVVRDAVIT SNGLLTDKYK YIQKLRESRE RIQFLSMSTK 

       490        500        510        520        530        540 
KKVLVLGSGY VSGPVLEYLS RGNNIEITLG SDMTNQMQQL SKKYDINTVN VTVGKQEDKL 

       550        560        570        580        590        600 
QSLVESQDLV ISLLPYVLHP VVAKACIDSK VNMVTASYIT PAMKELEKSV DDAGITVIGE 

       610        620        630        640        650        660 
LGLDPGLDHM LAMETIDKAK DLGATIESYV SYCGGLPAPE HSDNPLRYKF SWSPVGVLMN 

       670        680        690        700        710        720 
IMQPASYLLN GKVVNVTGGV SFLNSVTPMD YFPGLNLEGY PNRDSTKYAE IYGISSAHTL 

       730        740        750        760        770        780 
LRGTLRYKGY SKALNGFVKL GLINRETYPA LRPEANPLTW KQLLCDLVGI SRSSSCEKLK 

       790        800        810        820        830        840 
EVVFTKLGGD STQLEAAEWL GLLGDEQVPQ AESIVDAFSK HLVSKLSYGP EEKDMIVMRD 

       850        860        870        880        890        900 
SFGIRHPSGH LENKTIDLVV YGDFNGFSAM AKTVGLPTAM AAKMLLDGEI ETKGLMGPFS 

       910        920 
KEIYGPILER IKAEGIVFNT QSTIKL

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References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC128771 mRNA. Translation: AAI28772.1.
IPIIPI00858395.
RefSeqNP_001094433.1. NM_001100963.1.
UniGeneRn.198671.

3D structure databases

ProteinModelPortalA2VCW9.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000057271.

Proteomic databases

PaxDbA2VCW9.
PRIDEA2VCW9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID296925.
KEGGrno:296925.
UCSCRGD:1310811. rat.

Organism-specific databases

CTD10157.
RGD1310811. Aass.

Phylogenomic databases

eggNOGCOG1748.
HOVERGENHBG048688.
KOK14157.

Enzyme and pathway databases

UniPathwayUPA00868; UER00835.
UPA00868; UER00836.

Gene expression databases

GenevestigatorA2VCW9.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR016040. NAD(P)-bd_dom.
IPR005097. Saccharopine_DH/HSpermid_syn.
[Graphical view]
PfamPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PF03435. Saccharop_dh. 1 hit.
[Graphical view]
SMARTSM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]
PROSITEPS00836. ALADH_PNT_1. False negative.
PS00837. ALADH_PNT_2. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio641904.

Entry information

Entry nameAASS_RAT
AccessionPrimary (citable) accession number: A2VCW9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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