Reviewed, UniProtKB/Swiss-Prot A2VCW9 (AASS_RAT)

Names and origin

Protein names

Recommended name:
    Alpha-aminoadipic semialdehyde synthase, mitochondrial
Alternative name(s):
    LKR/SDH
Including the following 2 domains:
    1- Recommended name:
            Lysine ketoglutarate reductase
                Short name=LKR
                Short name=LOR
              EC=1.5.1.8
    2- Recommended name:
            Saccharopine dehydrogenase
                Short name=SDH
              EC=1.5.1.9

Gene names

Name:

Aass

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively By similarity. UniProtKB Q9UDR5
Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH. UniProtKB Q9UDR5 N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-glutamate + 2-aminoadipate 6-semialdehyde + NADH. UniProtKB Q9UDR5
Pathway	Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 1/6. Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6.
Subunit structure	Homodimer By similarity. UniProtKB Q9UDR5
Subcellular location	Mitochondrion By similarity. UniProtKB Q9UDR5
Sequence similarities	In the N-terminal section; belongs to the AlaDH/PNT family. In the C-terminal section; belongs to the saccharopine dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity Inferred from electronic annotation. Source: EC saccharopine dehydrogenase (NADP+, L-lysine-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Mitochondrion By similarity UniProtKB Q9UDR5
	Chain	33 – 926	894	Alpha-aminoadipic semialdehyde synthase, mitochondrial UniProtKB Q9UDR5		PRO_0000315868
Regions
	Region	33 – 455	423	Lysine-ketoglutarate reductase By similarity UniProtKB Q9UDR5
	Region	477 – 926	450	Saccharopine dehydrogenase By similarity UniProtKB Q9UDR5

Sequences

	Sequence		Length	Mass (Da)
	A2VCW9-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 4350DDB240B4075F	FASTA	926	102,908
	10 20 30 40 50 60 MLRAQRLRLA RLRACVSRGL HHKPVMALRR EDVNAWERRA PLAPKHIKGI TKLGYKVLIQ 70 80 90 100 110 120 PSNRRAIHDK EYVRAGGILQ EDITEACLIL GVKRPPEEKL MSKKTYAFFS HTIKAQEANM 130 140 150 160 170 180 SLLDEVLKQE IRLIDYEKMV DHRGSRIVAF GHWAGVAGMI NILHGMGLRL LALGHHTPFM 190 200 210 220 230 240 HLGMAHNYRN SSQAVQAVRD AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQEVFNELPC 250 260 270 280 290 300 EYVEPHELKE VSKTGDLRKV YGTVLSRHHH LVRKTDGVYD PVEYEKYPER YISRFNADIA 310 320 330 340 350 360 PYTTCLINGI YWEQNTPRLL TRQDAQSLLV PVKSSVVPVE GCPELPHKLV AICDISADTG 370 380 390 400 410 420 GSIDFMTECT TIERPFCMYD ADQHIIHDSV EGSGILMCSI DNLPAQLPIE ATEYFGDMLY 430 440 450 460 470 480 PYVEEMLLSD ASQPLESQNF SPVVRDAVIT SNGLLTDKYK YIQKLRESRE RIQFLSMSTK 490 500 510 520 530 540 KKVLVLGSGY VSGPVLEYLS RGNNIEITLG SDMTNQMQQL SKKYDINTVN VTVGKQEDKL 550 560 570 580 590 600 QSLVESQDLV ISLLPYVLHP VVAKACIDSK VNMVTASYIT PAMKELEKSV DDAGITVIGE 610 620 630 640 650 660 LGLDPGLDHM LAMETIDKAK DLGATIESYV SYCGGLPAPE HSDNPLRYKF SWSPVGVLMN 670 680 690 700 710 720 IMQPASYLLN GKVVNVTGGV SFLNSVTPMD YFPGLNLEGY PNRDSTKYAE IYGISSAHTL 730 740 750 760 770 780 LRGTLRYKGY SKALNGFVKL GLINRETYPA LRPEANPLTW KQLLCDLVGI SRSSSCEKLK 790 800 810 820 830 840 EVVFTKLGGD STQLEAAEWL GLLGDEQVPQ AESIVDAFSK HLVSKLSYGP EEKDMIVMRD 850 860 870 880 890 900 SFGIRHPSGH LENKTIDLVV YGDFNGFSAM AKTVGLPTAM AAKMLLDGEI ETKGLMGPFS 910 920 KEIYGPILER IKAEGIVFNT QSTIKL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC128771 mRNA. Translation: AAI28772.1.
IPI	IPI00858395.
RefSeq	NP_001094433.1.
UniGene	Rn.198671
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	296925.
KEGG	rno:296925.
Organism-specific databases
RGD	1310811. Aass.
Phylogenomic databases
HOVERGEN	A2VCW9.
Enzyme and pathway databases
BRENDA	1.5.1.8. 248. 1.5.1.9. 248.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR007886. Ala_DH/PNT_N. IPR005097. Saccharopine_DH. [Graphical view]
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. PF03435. Saccharop_dh. 1 hit. [Graphical view]
PROSITE	PS00836. ALADH_PNT_1. False negative. PS00837. ALADH_PNT_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	641904.

Entry information

Entry name

AASS_RAT

Accession

Primary (citable) accession number: A2VCW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 20, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)