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Protein

Alpha-aminoadipic semialdehyde synthase, mitochondrial

Gene

Aass

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively (By similarity).By similarity

Catalytic activityi

N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH.By similarity
N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADH.

Pathwayi: L-lysine degradation via saccharopine pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes glutaryl-CoA from L-lysine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Alpha-aminoadipic semialdehyde synthase, mitochondrial (Aass)
  2. Alpha-aminoadipic semialdehyde synthase, mitochondrial (Aass)
  3. no protein annotated in this organism
  4. Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (Aadat)
  5. no protein annotated in this organism
  6. Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (Dlst)
This subpathway is part of the pathway L-lysine degradation via saccharopine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutaryl-CoA from L-lysine, the pathway L-lysine degradation via saccharopine pathway and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00868; UER00835.
UPA00868; UER00836.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-aminoadipic semialdehyde synthase, mitochondrial
Alternative name(s):
LKR/SDH
Including the following 2 domains:
Lysine ketoglutarate reductase (EC:1.5.1.8)
Short name:
LKR
Short name:
LOR
Saccharopine dehydrogenase (EC:1.5.1.9)
Short name:
SDH
Gene namesi
Name:AassImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1310811. Aass.

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232MitochondrionBy similarityAdd
BLAST
Chaini33 – 926894Alpha-aminoadipic semialdehyde synthase, mitochondrialPRO_0000315868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481N6-acetyllysineBy similarity
Modified residuei52 – 521N6-acetyllysineBy similarity
Modified residuei56 – 561N6-acetyllysineBy similarity
Modified residuei93 – 931N6-acetyllysine; alternateBy similarity
Modified residuei93 – 931N6-succinyllysine; alternateBy similarity
Modified residuei128 – 1281N6-acetyllysineBy similarity
Modified residuei138 – 1381N6-acetyllysine; alternateBy similarity
Modified residuei138 – 1381N6-succinyllysine; alternateBy similarity
Modified residuei274 – 2741N6-succinyllysineBy similarity
Modified residuei286 – 2861N6-acetyllysine; alternateBy similarity
Modified residuei286 – 2861N6-succinyllysine; alternateBy similarity
Modified residuei333 – 3331N6-succinyllysineBy similarity
Modified residuei458 – 4581N6-acetyllysine; alternateBy similarity
Modified residuei458 – 4581N6-succinyllysine; alternateBy similarity
Modified residuei523 – 5231N6-acetyllysine; alternateBy similarity
Modified residuei523 – 5231N6-succinyllysine; alternateBy similarity
Modified residuei535 – 5351N6-acetyllysine; alternateBy similarity
Modified residuei535 – 5351N6-succinyllysine; alternateBy similarity
Modified residuei584 – 5841N6-acetyllysine; alternateBy similarity
Modified residuei584 – 5841N6-succinyllysine; alternateBy similarity
Modified residuei707 – 7071N6-acetyllysineBy similarity
Modified residuei732 – 7321N6-succinyllysineBy similarity
Modified residuei739 – 7391N6-acetyllysineBy similarity
Modified residuei761 – 7611N6-acetyllysine; alternateBy similarity
Modified residuei761 – 7611N6-succinyllysine; alternateBy similarity
Modified residuei778 – 7781N6-acetyllysineBy similarity
Modified residuei780 – 7801N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiA2VCW9.
PRIDEiA2VCW9.

PTM databases

iPTMnetiA2VCW9.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057271.

Structurei

3D structure databases

ProteinModelPortaliA2VCW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 455423Lysine-ketoglutarate reductaseBy similarityAdd
BLAST
Regioni477 – 926450Saccharopine dehydrogenaseBy similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the AlaDH/PNT family.Sequence analysis
In the C-terminal section; belongs to the saccharopine dehydrogenase family.Sequence analysis

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0172. Eukaryota.
COG1748. LUCA.
HOVERGENiHBG048688.
InParanoidiA2VCW9.
KOiK14157.
PhylomeDBiA2VCW9.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR016040. NAD(P)-bd_dom.
IPR032095. Sacchrp_dh_C.
IPR005097. Sacchrp_dh_NADP.
[Graphical view]
PfamiPF05222. AlaDh_PNT_N. 1 hit.
PF16653. Sacchrp_dh_C. 1 hit.
PF03435. Sacchrp_dh_NADP. 1 hit.
[Graphical view]
SMARTiSM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2VCW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRAQRLRLA RLRACVSRGL HHKPVMALRR EDVNAWERRA PLAPKHIKGI
60 70 80 90 100
TKLGYKVLIQ PSNRRAIHDK EYVRAGGILQ EDITEACLIL GVKRPPEEKL
110 120 130 140 150
MSKKTYAFFS HTIKAQEANM SLLDEVLKQE IRLIDYEKMV DHRGSRIVAF
160 170 180 190 200
GHWAGVAGMI NILHGMGLRL LALGHHTPFM HLGMAHNYRN SSQAVQAVRD
210 220 230 240 250
AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQEVFNELPC EYVEPHELKE
260 270 280 290 300
VSKTGDLRKV YGTVLSRHHH LVRKTDGVYD PVEYEKYPER YISRFNADIA
310 320 330 340 350
PYTTCLINGI YWEQNTPRLL TRQDAQSLLV PVKSSVVPVE GCPELPHKLV
360 370 380 390 400
AICDISADTG GSIDFMTECT TIERPFCMYD ADQHIIHDSV EGSGILMCSI
410 420 430 440 450
DNLPAQLPIE ATEYFGDMLY PYVEEMLLSD ASQPLESQNF SPVVRDAVIT
460 470 480 490 500
SNGLLTDKYK YIQKLRESRE RIQFLSMSTK KKVLVLGSGY VSGPVLEYLS
510 520 530 540 550
RGNNIEITLG SDMTNQMQQL SKKYDINTVN VTVGKQEDKL QSLVESQDLV
560 570 580 590 600
ISLLPYVLHP VVAKACIDSK VNMVTASYIT PAMKELEKSV DDAGITVIGE
610 620 630 640 650
LGLDPGLDHM LAMETIDKAK DLGATIESYV SYCGGLPAPE HSDNPLRYKF
660 670 680 690 700
SWSPVGVLMN IMQPASYLLN GKVVNVTGGV SFLNSVTPMD YFPGLNLEGY
710 720 730 740 750
PNRDSTKYAE IYGISSAHTL LRGTLRYKGY SKALNGFVKL GLINRETYPA
760 770 780 790 800
LRPEANPLTW KQLLCDLVGI SRSSSCEKLK EVVFTKLGGD STQLEAAEWL
810 820 830 840 850
GLLGDEQVPQ AESIVDAFSK HLVSKLSYGP EEKDMIVMRD SFGIRHPSGH
860 870 880 890 900
LENKTIDLVV YGDFNGFSAM AKTVGLPTAM AAKMLLDGEI ETKGLMGPFS
910 920
KEIYGPILER IKAEGIVFNT QSTIKL
Length:926
Mass (Da):102,908
Last modified:March 20, 2007 - v1
Checksum:i4350DDB240B4075F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC128771 mRNA. Translation: AAI28772.1.
RefSeqiNP_001094433.1. NM_001100963.1.
UniGeneiRn.198671.

Genome annotation databases

GeneIDi296925.
KEGGirno:296925.
UCSCiRGD:1310811. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC128771 mRNA. Translation: AAI28772.1.
RefSeqiNP_001094433.1. NM_001100963.1.
UniGeneiRn.198671.

3D structure databases

ProteinModelPortaliA2VCW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057271.

PTM databases

iPTMnetiA2VCW9.

Proteomic databases

PaxDbiA2VCW9.
PRIDEiA2VCW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi296925.
KEGGirno:296925.
UCSCiRGD:1310811. rat.

Organism-specific databases

CTDi10157.
RGDi1310811. Aass.

Phylogenomic databases

eggNOGiKOG0172. Eukaryota.
COG1748. LUCA.
HOVERGENiHBG048688.
InParanoidiA2VCW9.
KOiK14157.
PhylomeDBiA2VCW9.

Enzyme and pathway databases

UniPathwayiUPA00868; UER00835.
UPA00868; UER00836.

Miscellaneous databases

NextBioi641904.
PROiA2VCW9.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR016040. NAD(P)-bd_dom.
IPR032095. Sacchrp_dh_C.
IPR005097. Sacchrp_dh_NADP.
[Graphical view]
PfamiPF05222. AlaDh_PNT_N. 1 hit.
PF16653. Sacchrp_dh_C. 1 hit.
PF03435. Sacchrp_dh_NADP. 1 hit.
[Graphical view]
SMARTiSM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: KidneyImported.

Entry informationi

Entry nameiAASS_RAT
AccessioniPrimary (citable) accession number: A2VCW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: January 20, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.