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A2VCW5 (S38A5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sodium-coupled neutral amino acid transporter 5
Alternative name(s):
Solute carrier family 38 member 5
System N transporter 2
Gene names
Name:Slc38a5
Synonyms:Sn2, Snat5
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium. Ref.1 Ref.4

Enzyme regulation

Not inhibited by lithium By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.3.

Tissue specificity

Highly expressed in neocortex, hippocampus, striatum and spinal cord by astrocytes (at protein level). Expressed in brain, lung, stomach, liver, kidney, spleen and testis. Ref.1 Ref.3

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Biophysicochemical properties

Kinetic parameters:

KM=0.92 mM for serine (at pH 8.0) Ref.4

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglycine transmembrane transporter activity

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A2VCW5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A2VCW5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Sodium-coupled neutral amino acid transporter 5
PRO_0000312117

Regions

Topological domain1 – 5858Cytoplasmic Potential
Transmembrane59 – 8123Helical; Potential
Topological domain82 – 9716Extracellular Potential
Transmembrane98 – 11821Helical; Potential
Topological domain119 – 13517Cytoplasmic Potential
Transmembrane136 – 15621Helical; Potential
Topological domain157 – 17620Extracellular Potential
Transmembrane177 – 19721Helical; Potential
Topological domain198 – 2025Cytoplasmic Potential
Transmembrane203 – 22321Helical; Potential
Topological domain224 – 26441Extracellular Potential
Transmembrane265 – 28521Helical; Potential
Topological domain286 – 30217Cytoplasmic Potential
Transmembrane303 – 32321Helical; Potential
Topological domain324 – 34118Extracellular Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 38321Cytoplasmic Potential
Transmembrane384 – 40421Helical; Potential
Topological domain405 – 4062Extracellular Potential
Transmembrane407 – 42721Helical; Potential
Topological domain428 – 44619Cytoplasmic Potential
Transmembrane447 – 46721Helical; Potential
Topological domain468 – 47912Extracellular Potential

Amino acid modifications

Glycosylation2361N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 88Missing in isoform 2.
VSP_029704

Experimental info

Mutagenesis4791H → A: Modifies the transporter pH-sensitivity. Ref.4
Sequence conflict3921I → N in AAK69075. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: DC172C28CB7F5EDC

FASTA47952,436
        10         20         30         40         50         60 
MAISCAVGME MQEPKMNGTL STGAAAGYRQ EREGFLPTTH GPAPGRKPVQ FLDFEGKTSF 

        70         80         90        100        110        120 
GMSVFNLSNA IMGSGILGLA YAMAHTGVIF FLALLLCIAL LSSYSIHLLL TCASVVGIRA 

       130        140        150        160        170        180 
YEQLGQRAFG PAGKVVVAII ICLHNVGAMS SYLFIIKSEL PLVIGTFLHM DPEGDWFLKG 

       190        200        210        220        230        240 
NLLIILVSLL IILPLALMKH LGYLGYTSSL SLTCMLFFLI SVIYKKFQLG CVVSHNDTVV 

       250        260        270        280        290        300 
ESEPAPLQAF NSSCEAKLFT VDSQMSYTVP IMAFAFVCHP EVLPIYTELC CPTQRRMQAV 

       310        320        330        340        350        360 
ANMSIGAMFI MYGLTATFGY LTFYSTVKAE MLEMYTQEDL LILCVRLAVL LAVTLTVPVV 

       370        380        390        400        410        420 
LFPIRRALQQ LLFPSKAFSW PRHVAIALIL LILVNILVIC VPTIRDIFGF IGSTSAPSLI 

       430        440        450        460        470 
FILPSVFYLR IVPADMEPLF SWPKIQALCF GVLGVLFMAI SLGFMFANWA TGQSRMSGH

« Hide

Isoform 2 [UniParc].

Checksum: F72BB61356B43F1E
Show »

FASTA47151,703

References

« Hide 'large scale' references
[1]"Cloning and functional characterization of a new subtype of the amino acid transport system N."
Nakanishi T., Kekuda R., Fei Y.J., Hatanaka T., Sugawara M., Martindale R.G., Leibach F.H., Prasad P.D., Ganapathy V.
Am. J. Physiol. 281:C1757-C1768(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Amino acid transporter SNAT5 localizes to glial cells in the rat brain."
Cubelos B., Gonzalez-Gonzalez I.M., Gimenez C., Zafra F.
Glia 49:230-244(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]"Evidence for allosteric regulation of pH-sensitive System A (SNAT2) and System N (SNAT5) amino acid transporter activity involving a conserved histidine residue."
Baird F.E., Pinilla-Tenas J.J., Ogilvie W.L.J., Ganapathy V., Hundal H.S., Taylor P.M.
Biochem. J. 397:369-375(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF HIS-479, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF276870 mRNA. Translation: AAK69075.1.
BC128725 mRNA. Translation: AAI28726.1.
IPIIPI00208097.
IPI00877357.
RefSeqNP_620209.1. NM_138854.1.
UniGeneRn.81033.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000038781.

Protein family/group databases

TCDB2.A.18.6.8. amino acid/auxin permease (AAAP) family.

Proteomic databases

PaxDbA2VCW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000038068; ENSRNOP00000038781; ENSRNOG00000027767.
GeneID192208.
KEGGrno:192208.
UCSCRGD:620702. rat.

Organism-specific databases

CTD92745.
RGD620702. Slc38a5.

Phylogenomic databases

eggNOGNOG236441.
GeneTreeENSGT00650000093166.
HOGENOMHOG000013088.
HOVERGENHBG059571.
InParanoidA2VCW5.
KOK14992.
OMAWATGQSH.
OrthoDBEOG4H72BR.

Gene expression databases

GenevestigatorA2VCW5.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio622782.

Entry information

Entry nameS38A5_RAT
AccessionPrimary (citable) accession number: A2VCW5
Secondary accession number(s): Q91XR7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: March 20, 2007
Last modified: April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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