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A2VCW5

- S38A5_RAT

UniProt

A2VCW5 - S38A5_RAT

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Protein

Sodium-coupled neutral amino acid transporter 5

Gene

Slc38a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium.2 Publications

Enzyme regulationi

Not inhibited by lithium.By similarity

Kineticsi

  1. KM=0.92 mM for serine (at pH 8.0)1 Publication

GO - Molecular functioni

  1. amino acid transmembrane transporter activity Source: RGD
  2. glycine transmembrane transporter activity Source: RGD
  3. neutral amino acid transmembrane transporter activity Source: RGD

GO - Biological processi

  1. amino acid transmembrane transport Source: GOC
  2. glycine transport Source: RGD
  3. neutral amino acid transport Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_243822. Amino acid transport across the plasma membrane.

Protein family/group databases

TCDBi2.A.18.6.8. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-coupled neutral amino acid transporter 5
Alternative name(s):
Solute carrier family 38 member 5
System N transporter 2
Gene namesi
Name:Slc38a5
Synonyms:Sn2, Snat5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome X

Organism-specific databases

RGDi620702. Slc38a5.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5858CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei59 – 8123HelicalSequence AnalysisAdd
BLAST
Topological domaini82 – 9716ExtracellularSequence AnalysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence AnalysisAdd
BLAST
Topological domaini119 – 13517CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei136 – 15621HelicalSequence AnalysisAdd
BLAST
Topological domaini157 – 17620ExtracellularSequence AnalysisAdd
BLAST
Transmembranei177 – 19721HelicalSequence AnalysisAdd
BLAST
Topological domaini198 – 2025CytoplasmicSequence Analysis
Transmembranei203 – 22321HelicalSequence AnalysisAdd
BLAST
Topological domaini224 – 26441ExtracellularSequence AnalysisAdd
BLAST
Transmembranei265 – 28521HelicalSequence AnalysisAdd
BLAST
Topological domaini286 – 30217CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence AnalysisAdd
BLAST
Topological domaini363 – 38321CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei384 – 40421HelicalSequence AnalysisAdd
BLAST
Topological domaini405 – 4062ExtracellularSequence Analysis
Transmembranei407 – 42721HelicalSequence AnalysisAdd
BLAST
Topological domaini428 – 44619CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei447 – 46721HelicalSequence AnalysisAdd
BLAST
Topological domaini468 – 47912ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi479 – 4791H → A: Modifies the transporter pH-sensitivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Sodium-coupled neutral amino acid transporter 5PRO_0000312117Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiA2VCW5.

Expressioni

Tissue specificityi

Highly expressed in neocortex, hippocampus, striatum and spinal cord by astrocytes (at protein level). Expressed in brain, lung, stomach, liver, kidney, spleen and testis.2 Publications

Gene expression databases

GenevestigatoriA2VCW5.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038781.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG236441.
GeneTreeiENSGT00760000119147.
HOGENOMiHOG000013088.
HOVERGENiHBG059571.
InParanoidiA2VCW5.
KOiK14992.
OMAiNTSCEAQ.
OrthoDBiEOG7WHH9J.
PhylomeDBiA2VCW5.
TreeFamiTF328787.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A2VCW5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAISCAVGME MQEPKMNGTL STGAAAGYRQ EREGFLPTTH GPAPGRKPVQ
60 70 80 90 100
FLDFEGKTSF GMSVFNLSNA IMGSGILGLA YAMAHTGVIF FLALLLCIAL
110 120 130 140 150
LSSYSIHLLL TCASVVGIRA YEQLGQRAFG PAGKVVVAII ICLHNVGAMS
160 170 180 190 200
SYLFIIKSEL PLVIGTFLHM DPEGDWFLKG NLLIILVSLL IILPLALMKH
210 220 230 240 250
LGYLGYTSSL SLTCMLFFLI SVIYKKFQLG CVVSHNDTVV ESEPAPLQAF
260 270 280 290 300
NSSCEAKLFT VDSQMSYTVP IMAFAFVCHP EVLPIYTELC CPTQRRMQAV
310 320 330 340 350
ANMSIGAMFI MYGLTATFGY LTFYSTVKAE MLEMYTQEDL LILCVRLAVL
360 370 380 390 400
LAVTLTVPVV LFPIRRALQQ LLFPSKAFSW PRHVAIALIL LILVNILVIC
410 420 430 440 450
VPTIRDIFGF IGSTSAPSLI FILPSVFYLR IVPADMEPLF SWPKIQALCF
460 470
GVLGVLFMAI SLGFMFANWA TGQSRMSGH
Length:479
Mass (Da):52,436
Last modified:March 20, 2007 - v1
Checksum:iDC172C28CB7F5EDC
GO
Isoform 2 (identifier: A2VCW5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:471
Mass (Da):51,703
Checksum:iF72BB61356B43F1E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti392 – 3921I → N in AAK69075. (PubMed:11698233)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88Missing in isoform 2. 1 PublicationVSP_029704

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276870 mRNA. Translation: AAK69075.1.
BC128725 mRNA. Translation: AAI28726.1.
RefSeqiNP_620209.1. NM_138854.1.
UniGeneiRn.81033.

Genome annotation databases

EnsembliENSRNOT00000038068; ENSRNOP00000038781; ENSRNOG00000027767. [A2VCW5-2]
GeneIDi192208.
KEGGirno:192208.
UCSCiRGD:620702. rat. [A2VCW5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276870 mRNA. Translation: AAK69075.1 .
BC128725 mRNA. Translation: AAI28726.1 .
RefSeqi NP_620209.1. NM_138854.1.
UniGenei Rn.81033.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000038781.

Protein family/group databases

TCDBi 2.A.18.6.8. the amino acid/auxin permease (aaap) family.

Proteomic databases

PaxDbi A2VCW5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000038068 ; ENSRNOP00000038781 ; ENSRNOG00000027767 . [A2VCW5-2 ]
GeneIDi 192208.
KEGGi rno:192208.
UCSCi RGD:620702. rat. [A2VCW5-1 ]

Organism-specific databases

CTDi 92745.
RGDi 620702. Slc38a5.

Phylogenomic databases

eggNOGi NOG236441.
GeneTreei ENSGT00760000119147.
HOGENOMi HOG000013088.
HOVERGENi HBG059571.
InParanoidi A2VCW5.
KOi K14992.
OMAi NTSCEAQ.
OrthoDBi EOG7WHH9J.
PhylomeDBi A2VCW5.
TreeFami TF328787.

Enzyme and pathway databases

Reactomei REACT_243822. Amino acid transport across the plasma membrane.

Miscellaneous databases

NextBioi 622782.
PROi A2VCW5.

Gene expression databases

Genevestigatori A2VCW5.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional characterization of a new subtype of the amino acid transport system N."
    Nakanishi T., Kekuda R., Fei Y.J., Hatanaka T., Sugawara M., Martindale R.G., Leibach F.H., Prasad P.D., Ganapathy V.
    Am. J. Physiol. 281:C1757-C1768(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Amino acid transporter SNAT5 localizes to glial cells in the rat brain."
    Cubelos B., Gonzalez-Gonzalez I.M., Gimenez C., Zafra F.
    Glia 49:230-244(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "Evidence for allosteric regulation of pH-sensitive System A (SNAT2) and System N (SNAT5) amino acid transporter activity involving a conserved histidine residue."
    Baird F.E., Pinilla-Tenas J.J., Ogilvie W.L.J., Ganapathy V., Hundal H.S., Taylor P.M.
    Biochem. J. 397:369-375(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF HIS-479, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiS38A5_RAT
AccessioniPrimary (citable) accession number: A2VCW5
Secondary accession number(s): Q91XR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: March 20, 2007
Last modified: November 26, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3