Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A2VCW5

- S38A5_RAT

UniProt

A2VCW5 - S38A5_RAT

Protein

Sodium-coupled neutral amino acid transporter 5

Gene

Slc38a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium.2 Publications

    Enzyme regulationi

    Not inhibited by lithium.By similarity

    Kineticsi

    1. KM=0.92 mM for serine (at pH 8.0)1 Publication

    GO - Molecular functioni

    1. amino acid transmembrane transporter activity Source: RGD
    2. glycine transmembrane transporter activity Source: RGD
    3. neutral amino acid transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. amino acid transmembrane transport Source: GOC
    2. glycine transport Source: RGD
    3. neutral amino acid transport Source: RGD

    Protein family/group databases

    TCDBi2.A.18.6.8. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled neutral amino acid transporter 5
    Alternative name(s):
    Solute carrier family 38 member 5
    System N transporter 2
    Gene namesi
    Name:Slc38a5
    Synonyms:Sn2, Snat5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome X

    Organism-specific databases

    RGDi620702. Slc38a5.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi479 – 4791H → A: Modifies the transporter pH-sensitivity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Sodium-coupled neutral amino acid transporter 5PRO_0000312117Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiA2VCW5.

    Expressioni

    Tissue specificityi

    Highly expressed in neocortex, hippocampus, striatum and spinal cord by astrocytes (at protein level). Expressed in brain, lung, stomach, liver, kidney, spleen and testis.2 Publications

    Gene expression databases

    GenevestigatoriA2VCW5.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000038781.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5858CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini82 – 9716ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini119 – 13517CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini157 – 17620ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini198 – 2025CytoplasmicSequence Analysis
    Topological domaini224 – 26441ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini286 – 30217CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini363 – 38321CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini405 – 4062ExtracellularSequence Analysis
    Topological domaini428 – 44619CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini468 – 47912ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei59 – 8123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei98 – 11821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei177 – 19721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei203 – 22321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei407 – 42721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei447 – 46721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG236441.
    GeneTreeiENSGT00650000093166.
    HOGENOMiHOG000013088.
    HOVERGENiHBG059571.
    InParanoidiA2VCW5.
    KOiK14992.
    OMAiNTSCEAQ.
    OrthoDBiEOG7WHH9J.
    PhylomeDBiA2VCW5.
    TreeFamiTF328787.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A2VCW5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAISCAVGME MQEPKMNGTL STGAAAGYRQ EREGFLPTTH GPAPGRKPVQ    50
    FLDFEGKTSF GMSVFNLSNA IMGSGILGLA YAMAHTGVIF FLALLLCIAL 100
    LSSYSIHLLL TCASVVGIRA YEQLGQRAFG PAGKVVVAII ICLHNVGAMS 150
    SYLFIIKSEL PLVIGTFLHM DPEGDWFLKG NLLIILVSLL IILPLALMKH 200
    LGYLGYTSSL SLTCMLFFLI SVIYKKFQLG CVVSHNDTVV ESEPAPLQAF 250
    NSSCEAKLFT VDSQMSYTVP IMAFAFVCHP EVLPIYTELC CPTQRRMQAV 300
    ANMSIGAMFI MYGLTATFGY LTFYSTVKAE MLEMYTQEDL LILCVRLAVL 350
    LAVTLTVPVV LFPIRRALQQ LLFPSKAFSW PRHVAIALIL LILVNILVIC 400
    VPTIRDIFGF IGSTSAPSLI FILPSVFYLR IVPADMEPLF SWPKIQALCF 450
    GVLGVLFMAI SLGFMFANWA TGQSRMSGH 479
    Length:479
    Mass (Da):52,436
    Last modified:March 20, 2007 - v1
    Checksum:iDC172C28CB7F5EDC
    GO
    Isoform 2 (identifier: A2VCW5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-8: Missing.

    Show »
    Length:471
    Mass (Da):51,703
    Checksum:iF72BB61356B43F1E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti392 – 3921I → N in AAK69075. (PubMed:11698233)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 88Missing in isoform 2. 1 PublicationVSP_029704

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF276870 mRNA. Translation: AAK69075.1.
    BC128725 mRNA. Translation: AAI28726.1.
    RefSeqiNP_620209.1. NM_138854.1.
    UniGeneiRn.81033.

    Genome annotation databases

    EnsembliENSRNOT00000038068; ENSRNOP00000038781; ENSRNOG00000027767. [A2VCW5-2]
    GeneIDi192208.
    KEGGirno:192208.
    UCSCiRGD:620702. rat. [A2VCW5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF276870 mRNA. Translation: AAK69075.1 .
    BC128725 mRNA. Translation: AAI28726.1 .
    RefSeqi NP_620209.1. NM_138854.1.
    UniGenei Rn.81033.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000038781.

    Protein family/group databases

    TCDBi 2.A.18.6.8. the amino acid/auxin permease (aaap) family.

    Proteomic databases

    PaxDbi A2VCW5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000038068 ; ENSRNOP00000038781 ; ENSRNOG00000027767 . [A2VCW5-2 ]
    GeneIDi 192208.
    KEGGi rno:192208.
    UCSCi RGD:620702. rat. [A2VCW5-1 ]

    Organism-specific databases

    CTDi 92745.
    RGDi 620702. Slc38a5.

    Phylogenomic databases

    eggNOGi NOG236441.
    GeneTreei ENSGT00650000093166.
    HOGENOMi HOG000013088.
    HOVERGENi HBG059571.
    InParanoidi A2VCW5.
    KOi K14992.
    OMAi NTSCEAQ.
    OrthoDBi EOG7WHH9J.
    PhylomeDBi A2VCW5.
    TreeFami TF328787.

    Miscellaneous databases

    NextBioi 622782.
    PROi A2VCW5.

    Gene expression databases

    Genevestigatori A2VCW5.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional characterization of a new subtype of the amino acid transport system N."
      Nakanishi T., Kekuda R., Fei Y.J., Hatanaka T., Sugawara M., Martindale R.G., Leibach F.H., Prasad P.D., Ganapathy V.
      Am. J. Physiol. 281:C1757-C1768(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "Amino acid transporter SNAT5 localizes to glial cells in the rat brain."
      Cubelos B., Gonzalez-Gonzalez I.M., Gimenez C., Zafra F.
      Glia 49:230-244(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    4. "Evidence for allosteric regulation of pH-sensitive System A (SNAT2) and System N (SNAT5) amino acid transporter activity involving a conserved histidine residue."
      Baird F.E., Pinilla-Tenas J.J., Ogilvie W.L.J., Ganapathy V., Hundal H.S., Taylor P.M.
      Biochem. J. 397:369-375(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF HIS-479, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiS38A5_RAT
    AccessioniPrimary (citable) accession number: A2VCW5
    Secondary accession number(s): Q91XR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3