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Protein

Sodium-coupled neutral amino acid transporter 5

Gene

Slc38a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium.2 Publications

Enzyme regulationi

Not inhibited by lithium.By similarity

Kineticsi

  1. KM=0.92 mM for serine (at pH 8.0)1 Publication

    GO - Molecular functioni

    • amino acid transmembrane transporter activity Source: RGD
    • glycine transmembrane transporter activity Source: RGD
    • neutral amino acid transmembrane transporter activity Source: RGD

    GO - Biological processi

    • amino acid transmembrane transport Source: GOC
    • glycine transport Source: RGD
    • neutral amino acid transport Source: RGD
    Complete GO annotation...

    Enzyme and pathway databases

    ReactomeiREACT_311758. Amino acid transport across the plasma membrane.

    Protein family/group databases

    TCDBi2.A.18.6.8. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled neutral amino acid transporter 5
    Alternative name(s):
    Solute carrier family 38 member 5
    System N transporter 2
    Gene namesi
    Name:Slc38a5
    Synonyms:Sn2, Snat5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494 Componenti: Chromosome X

    Organism-specific databases

    RGDi620702. Slc38a5.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5858CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei59 – 8123HelicalSequence AnalysisAdd
    BLAST
    Topological domaini82 – 9716ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei98 – 11821HelicalSequence AnalysisAdd
    BLAST
    Topological domaini119 – 13517CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST
    Topological domaini157 – 17620ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei177 – 19721HelicalSequence AnalysisAdd
    BLAST
    Topological domaini198 – 2025CytoplasmicSequence Analysis
    Transmembranei203 – 22321HelicalSequence AnalysisAdd
    BLAST
    Topological domaini224 – 26441ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Topological domaini286 – 30217CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Topological domaini363 – 38321CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST
    Topological domaini405 – 4062ExtracellularSequence Analysis
    Transmembranei407 – 42721HelicalSequence AnalysisAdd
    BLAST
    Topological domaini428 – 44619CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei447 – 46721HelicalSequence AnalysisAdd
    BLAST
    Topological domaini468 – 47912ExtracellularSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi479 – 4791H → A: Modifies the transporter pH-sensitivity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Sodium-coupled neutral amino acid transporter 5PRO_0000312117Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiA2VCW5.

    Expressioni

    Tissue specificityi

    Highly expressed in neocortex, hippocampus, striatum and spinal cord by astrocytes (at protein level). Expressed in brain, lung, stomach, liver, kidney, spleen and testis.2 Publications

    Gene expression databases

    GenevisibleiA2VCW5. RN.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000038781.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG236441.
    GeneTreeiENSGT00760000119147.
    HOGENOMiHOG000013088.
    HOVERGENiHBG059571.
    InParanoidiA2VCW5.
    KOiK14992.
    OMAiEMLEMYT.
    OrthoDBiEOG7WHH9J.
    PhylomeDBiA2VCW5.
    TreeFamiTF328787.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: A2VCW5-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MAISCAVGME MQEPKMNGTL STGAAAGYRQ EREGFLPTTH GPAPGRKPVQ
    60 70 80 90 100
    FLDFEGKTSF GMSVFNLSNA IMGSGILGLA YAMAHTGVIF FLALLLCIAL
    110 120 130 140 150
    LSSYSIHLLL TCASVVGIRA YEQLGQRAFG PAGKVVVAII ICLHNVGAMS
    160 170 180 190 200
    SYLFIIKSEL PLVIGTFLHM DPEGDWFLKG NLLIILVSLL IILPLALMKH
    210 220 230 240 250
    LGYLGYTSSL SLTCMLFFLI SVIYKKFQLG CVVSHNDTVV ESEPAPLQAF
    260 270 280 290 300
    NSSCEAKLFT VDSQMSYTVP IMAFAFVCHP EVLPIYTELC CPTQRRMQAV
    310 320 330 340 350
    ANMSIGAMFI MYGLTATFGY LTFYSTVKAE MLEMYTQEDL LILCVRLAVL
    360 370 380 390 400
    LAVTLTVPVV LFPIRRALQQ LLFPSKAFSW PRHVAIALIL LILVNILVIC
    410 420 430 440 450
    VPTIRDIFGF IGSTSAPSLI FILPSVFYLR IVPADMEPLF SWPKIQALCF
    460 470
    GVLGVLFMAI SLGFMFANWA TGQSRMSGH
    Length:479
    Mass (Da):52,436
    Last modified:March 20, 2007 - v1
    Checksum:iDC172C28CB7F5EDC
    GO
    Isoform 2 (identifier: A2VCW5-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-8: Missing.

    Show »
    Length:471
    Mass (Da):51,703
    Checksum:iF72BB61356B43F1E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti392 – 3921I → N in AAK69075 (PubMed:11698233).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 88Missing in isoform 2. 1 PublicationVSP_029704

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF276870 mRNA. Translation: AAK69075.1.
    BC128725 mRNA. Translation: AAI28726.1.
    RefSeqiNP_620209.1. NM_138854.1.
    UniGeneiRn.81033.

    Genome annotation databases

    EnsembliENSRNOT00000079664; ENSRNOP00000073818; ENSRNOG00000027767. [A2VCW5-2]
    GeneIDi192208.
    KEGGirno:192208.
    UCSCiRGD:620702. rat. [A2VCW5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF276870 mRNA. Translation: AAK69075.1.
    BC128725 mRNA. Translation: AAI28726.1.
    RefSeqiNP_620209.1. NM_138854.1.
    UniGeneiRn.81033.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000038781.

    Protein family/group databases

    TCDBi2.A.18.6.8. the amino acid/auxin permease (aaap) family.

    Proteomic databases

    PaxDbiA2VCW5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000079664; ENSRNOP00000073818; ENSRNOG00000027767. [A2VCW5-2]
    GeneIDi192208.
    KEGGirno:192208.
    UCSCiRGD:620702. rat. [A2VCW5-1]

    Organism-specific databases

    CTDi92745.
    RGDi620702. Slc38a5.

    Phylogenomic databases

    eggNOGiNOG236441.
    GeneTreeiENSGT00760000119147.
    HOGENOMiHOG000013088.
    HOVERGENiHBG059571.
    InParanoidiA2VCW5.
    KOiK14992.
    OMAiEMLEMYT.
    OrthoDBiEOG7WHH9J.
    PhylomeDBiA2VCW5.
    TreeFamiTF328787.

    Enzyme and pathway databases

    ReactomeiREACT_311758. Amino acid transport across the plasma membrane.

    Miscellaneous databases

    NextBioi622782.
    PROiA2VCW5.

    Gene expression databases

    GenevisibleiA2VCW5. RN.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and functional characterization of a new subtype of the amino acid transport system N."
      Nakanishi T., Kekuda R., Fei Y.J., Hatanaka T., Sugawara M., Martindale R.G., Leibach F.H., Prasad P.D., Ganapathy V.
      Am. J. Physiol. 281:C1757-C1768(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "Amino acid transporter SNAT5 localizes to glial cells in the rat brain."
      Cubelos B., Gonzalez-Gonzalez I.M., Gimenez C., Zafra F.
      Glia 49:230-244(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    4. "Evidence for allosteric regulation of pH-sensitive System A (SNAT2) and System N (SNAT5) amino acid transporter activity involving a conserved histidine residue."
      Baird F.E., Pinilla-Tenas J.J., Ogilvie W.L.J., Ganapathy V., Hundal H.S., Taylor P.M.
      Biochem. J. 397:369-375(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF HIS-479, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiS38A5_RAT
    AccessioniPrimary (citable) accession number: A2VCW5
    Secondary accession number(s): Q91XR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: March 20, 2007
    Last modified: July 22, 2015
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.