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Protein

Phospholipase A1

Gene
N/A
Organism
Polybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of phosphatidylcholine with phospholipase A1 (EC 3.1.1.32) activity. Has weak hemolytic activity.

Catalytic activityi

Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei156NucleophileBy similarity1
Active sitei184Charge relay systemPROSITE-ProRule annotation1
Active sitei248Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cytolysis, Hemolysis, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.32. 9385.

Protein family/group databases

ESTHERipolpi-pa1. Phospholipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A1 (EC:3.1.1.32)
Alternative name(s):
Allergen: Poly p 1
OrganismiPolybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp)
Taxonomic identifieri291283 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaePolistinaeEpiponiniPolybia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3608. Poly p 1.
3609. Poly p 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_500022375519 – 322Phospholipase A1Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 263By similarity
Disulfide bondi106 ↔ 246By similarity
Disulfide bondi195 ↔ 238By similarity
Disulfide bondi200 ↔ 283By similarity
Disulfide bondi258 ↔ 264By similarity
Disulfide bondi290 ↔ 315By similarity

Post-translational modificationi

Contains six disulfide bonds.1 Publication
Is not glycosylated.1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA2VBC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd00707. Pancreat_lipase_like. 1 hit.
Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002334. Allerg_PlipaseA1.
IPR013818. Lipase/vitellogenin.
IPR033906. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PRINTSiPR00825. DOLALLERGEN.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2VBC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFKYSILFI CFGTLDRGLI PECPFNEYDI LFFVYTRQQR DGIVLTEETL
60 70 80 90 100
QNYDLFKKST ISRQVVFIDH GFLSNGNNEN FIAMAKALIE KDNFLVISVD
110 120 130 140 150
WKKGACNAFA STLDYLGYST AVGNTRHVGK YVADFTKLLV EQYKVSMSNI
160 170 180 190 200
RLIGHSLGAH TSGFAGKEVQ ELKLNKYSNI DGLDPAGPSF DSNDCPERLC
210 220 230 240 250
ETDAEYVQII HTSNILGVYS KIGTVDFYMN YGSHQPGCGR FFSPSCSHTK
260 270 280 290 300
AVKYLTECIK HECCLIGTPW KKYFSTPKPI SQCTKDTCVC VGLNAKSYPA
310 320
RGSFYVPVEA TAPYCHNEGI KL
Length:322
Mass (Da):36,082
Last modified:March 20, 2007 - v1
Checksum:i64A39D6D33AD86A8
GO

Mass spectrometryi

Molecular mass is 33961 Da from positions 19 - 322. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF101736 mRNA. Translation: ABN13879.1.
AM491805 mRNA. Translation: CAM33429.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF101736 mRNA. Translation: ABN13879.1.
AM491805 mRNA. Translation: CAM33429.1.

3D structure databases

ProteinModelPortaliA2VBC4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3608. Poly p 1.
3609. Poly p 1.0101.
ESTHERipolpi-pa1. Phospholipase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.1.32. 9385.

Family and domain databases

CDDicd00707. Pancreat_lipase_like. 1 hit.
Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002334. Allerg_PlipaseA1.
IPR013818. Lipase/vitellogenin.
IPR033906. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PRINTSiPR00825. DOLALLERGEN.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA1_POLPI
AccessioniPrimary (citable) accession number: A2VBC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: November 30, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.