A2V735 (TPM_CHIOP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tropomyosin

Alternative name(s):
Tropomyosin, slow-tonic isoform
Short name=Tm-Chio-tonic

Gene names

Name:

TM1

Organism

Chionoecetes opilio (Crab-beetle)

Taxonomic identifier

41210 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Brachyura › Eubrachyura › Heterotremata › Majoidea › Majidae › Chionoecetes

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tropomyosin, in association with the troponin complex, plays a central role in the calcium dependent regulation of muscle contraction By similarity. UniProtKB Q22866
Subunit structure	Homodimer. Ref.2
Tissue specificity	Expressed in leg muscle and chest protection muscle (at protein level). Ref.1
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Allergenic properties	Causes an allergic reaction in human. Ref.1 Ref.2
Sequence similarities	Belongs to the tropomyosin family.
Mass spectrometry	Molecular mass is 32733±6.5 Da from positions 1 - 284. Determined by MALDI. Ref.2

Ontologies

Keywords
Disease	Allergen
Domain	Coiled coil Repeat
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Tropomyosin		PRO_0000398787
Regions
	Coiled coil	1 – 284	284	By similarity UniProtKB O44119
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A2V735 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 357A164032C6B96E	FASTA	284	32,675
	10 20 30 40 50 60 MDAIKKKMQA MKLEKDNAMD KADTLEQQNK EANLRAEKTE EEIRANQKKS QLVENELDHA 70 80 90 100 110 120 QEQLSAATHK LVEKEKAFAN AEGEVAALNR RIQLLEEDLE RSEERLNTAT TKLAEASQAA 130 140 150 160 170 180 DESERMRKVL ENRSLSDEER MDALENQLKE ARFLAEEADR KYDEVARKLA MVEADLERAE 190 200 210 220 230 240 ERAESGESKI VELEEELRVV GNNLKSLEVS EEKANQREET YKEQIKTLAN KLKAAEARAE 250 260 270 280 FAERSVQKLQ KEVDRLEDEL VNEKEKYKNI ADEMDQAFSE LSGF

References

[1]

"Molecular cloning of tropomyosins identified as allergens in six species of crustaceans." Motoyama K., Suma Y., Ishizaki S., Nagashima Y., Shiomi K. J. Agric. Food Chem. 55:985-991(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, ALLERGEN. Tissue: Muscle.

[2]

"Characterization and de novo sequencing of snow crab tropomyosin enzymatic peptides by both electrospary ionization and matrix-assisted laser desorption ionization QqToF tandemmass spectrometry." Abdel Rahman A.M., Lopata A.L., O'Hehir R.E., Robinson J.J., Banoub J.H., Helleur R.J. J. Mass Spectrom. 45:372-381(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-284, SUBUNIT, MASS SPECTROMETRY, ALLERGENICITY, ACETYLATION AT MET-1. Tissue: Muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB270634 mRNA. Translation: BAF47267.1.
3D structure databases
ProteinModelPortal	A2V735.
SMR	A2V735. Positions 1-283.
ModBase	Search...
Protein family/group databases
Allergome	3807. Chi o 1. 4087. Chi o 1.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000533. Tropomyosin. [Graphical view]
Pfam	PF00261. Tropomyosin. 1 hit. [Graphical view]
PRINTS	PR00194. TROPOMYOSIN.
PROSITE	PS00326. TROPOMYOSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPM_CHIOP

Accession

Primary (citable) accession number: A2V735

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	March 20, 2007
Last modified:	October 31, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)