Reviewed, UniProtKB/Swiss-Prot A2TIL1 (GSHR_CALJA)

Names and origin

Protein names

Recommended name:
    Glutathione reductase, mitochondrial
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:

GSR

Organism

Callithrix jacchus (Common marmoset)

Taxonomic identifier

9483 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP(+) = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	MitochondrionBy similarity. CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: A2TIL1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic (identifier: A2TIL1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Notes: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion Potential
	Chain	44 – 522	479	Glutathione reductase, mitochondrial		PRO_0000314948
Regions
	Nucleotide binding	94 – 102	9	FAD By similarity
Sites
	Active site	511	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	65	1	Phosphotyrosine By similarity
	Disulfide bond	102 ↔ 107		Redox-active By similarity
	Disulfide bond	134		Interchain By similarity
Natural variations
	Alternative sequence	1 – 43	43	Missing in isoform Cytoplasmic.		VSP_030441

Sequences

	Sequence		Length	Mass (Da)
	Isoform Mitochondrial [UniParc]. Last modified March 6, 2007. Version 1. Checksum: A952357C8E8E0E86	FASTA	522	56,193
	10 20 30 40 50 60 MALLPRALSS GGRPSWRRAA RASRGFPLPL PFPAAATHAL SRAMACRQEP QPQGPPPSAG 70 80 90 100 110 120 AVVSYDYLVI GGGSGGLASA RRAAELGARA AVVESHKLGG TCVNVGCVPK KVMWNTAVHS 130 140 150 160 170 180 EFLHDHGDYG FSSCEGKFNW RVIKEKRDTY VSRLNTIYQN NLTKAHIEII HGHAVFTSDT 190 200 210 220 230 240 KPTIEVSGRK YTAPHILIAT GGMPSSPHES QIPGASLGIT SDGFFELEEL PSRSVIVGAG 250 260 270 280 290 300 YIAVEIAGIL SALGSKTSLM IRHDKVLRSF DSMISTNCTE ELENAGVEVL KFSQVKEVKK 310 320 330 340 350 360 TSSGLEVSLV TAVPGRLPVM TTISDVDCLL WAIGRDPNSK GLSLNKLGIK TDDKGHIIVD 370 380 390 400 410 420 EFQNTNVKGI YAVGDVCGKA LLTPVAIAAG RKLAHRLFEN KEDSKLDYNN IPTVVFSHPP 430 440 450 460 470 480 IGTVGLTEDE AIHKYGKENV KIYSTSFTPM YHAVTKRKTK CVMKMVCAYE EEKVVGIHMQ 490 500 510 520 GLGCDEMLQG FAVAVKMGAT KADFDNTVAI HPTSSEELVP LR
	Isoform Cytoplasmic [UniParc]. Checksum: 49B2B4DA8179C17C		479	51,670
	10 20 30 40 50 60 MACRQEPQPQ GPPPSAGAVV SYDYLVIGGG SGGLASARRA AELGARAAVV ESHKLGGTCV 70 80 90 100 110 120 NVGCVPKKVM WNTAVHSEFL HDHGDYGFSS CEGKFNWRVI KEKRDTYVSR LNTIYQNNLT 130 140 150 160 170 180 KAHIEIIHGH AVFTSDTKPT IEVSGRKYTA PHILIATGGM PSSPHESQIP GASLGITSDG 190 200 210 220 230 240 FFELEELPSR SVIVGAGYIA VEIAGILSAL GSKTSLMIRH DKVLRSFDSM ISTNCTEELE 250 260 270 280 290 300 NAGVEVLKFS QVKEVKKTSS GLEVSLVTAV PGRLPVMTTI SDVDCLLWAI GRDPNSKGLS 310 320 330 340 350 360 LNKLGIKTDD KGHIIVDEFQ NTNVKGIYAV GDVCGKALLT PVAIAAGRKL AHRLFENKED 370 380 390 400 410 420 SKLDYNNIPT VVFSHPPIGT VGLTEDEAIH KYGKENVKIY STSFTPMYHA VTKRKTKCVM 430 440 450 460 470 KMVCAYEEEK VVGIHMQGLG CDEMLQGFAV AVKMGATKAD FDNTVAIHPT SSEELVPLR

References

[1]	"Free radical scavenging enzymes in Callithrix jacchus." Atanasova S., von Ahsen N., Schlumbohm C., Wieland E., Oellerich M., Armstrong V. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF203010 mRNA. Translation: ABN05297.1.
3D structure databases
SMR	A2TIL1. Positions 62-522.
ModBase	Search...
Phylogenomic databases
HOVERGEN	A2TIL1.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006322. Glut_reduct_1. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01421. gluta_reduc_1. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_CALJA

Accession

Primary (citable) accession number: A2TIL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)