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A2T3R5

- VP2_ROTSH

UniProt

A2T3R5 - VP2_ROTSH

Protein

Inner capsid protein VP2

Gene
N/A
Organism
Rotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 24 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inner capsid protein VP2
    OrganismiRotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58))
    Taxonomic identifieri450149 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiCercopithecus pygerythrus (Vervet monkey) [TaxID: 60710]
    ProteomesiUP000001119: Genome

    Subcellular locationi

    Virion Curated
    Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

    GO - Cellular componenti

    1. viral inner capsid Source: UniProtKB-KW
    2. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Capsid protein, Inner capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 882882Inner capsid protein VP2PRO_0000367813Add
    BLAST

    Interactioni

    Subunit structurei

    Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.By similarityCurated

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9494Interaction with VP1 and VP3By similarityAdd
    BLAST
    Regioni396 – 41621HydrophobicAdd
    BLAST
    Regioni424 – 44421HydrophobicAdd
    BLAST
    Regioni538 – 55922Leucine-zipper 1Add
    BLAST
    Regioni667 – 68822Leucine-zipper 2Add
    BLAST

    Domaini

    The N-terminus is involved in VP1 binding.By similarity

    Sequence similaritiesi

    Belongs to the rotavirus VP2 family.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR007779. Rotavirus_VP2.
    [Graphical view]
    PfamiPF05087. Rota_VP2. 1 hit.
    [Graphical view]
    ProDomiPD008866. Rota_VP2. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    A2T3R5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYRKRGARR ETNLKQDERM QEKEDSKNIN NDSPKSQLSE KVLSKKEEII    50
    TDNQEEVKIS DEVKKSNKEE SKQLLEVLKT KEEHQKEVQY EILQKTIPTF 100
    EPKESILKKL EDIKPEQAKK QTKLFRIFEP KQLPIYRANG ERELRNRWYW 150
    KLKRDTLPDG DYDVREYFLN LYDQVLMEMP DYLLLKDMAV ENKNSRDAGK 200
    VVDSETAAIC DAIFQDEETE GAVRRFIAEM RQRVQADRNV VNYPSILHPI 250
    DHAFNEYFLQ HQLVEPLNND IIFNYIPERI RNDVNYILNM DRNLPSTARY 300
    IRPNLLQDRL NLHDNFESLW DTITTSNYIL ARSVVPDLKE LVSTEAQIQK 350
    MSQDLQLEAL TIQSETQFLT GINSQAANDC FKTLIAAMLS QRTMSLDFVT 400
    TNYMSLISGM WLLTVIPNDM FIRESLVACQ LAIINTIVYP AFGMQRMHYR 450
    NGDPQTPFQI AEQQIQNFQV ANWLHFVNYN QFRQVVIDGV LNQVLNDNIR 500
    NGHVVNQLME ALMQLSRQQF PTMPVDYKRS IQRGILLLSN RLGQLVDLTR 550
    LLSYNYETLM ACITMNMQHV QTLTTEKLQL TSVTSLCMLI GNATVIPSPQ 600
    TLFHYYNVNV NFHSNYNERI NDAVAIITAA NRLNLYQKKM KSIVEDFLKR 650
    LQIFDVARVP DDQMYRLRDR LRLLPVEIRR LDIFNLIAMN MEQIERASDK 700
    IAQGVIIAYR DMQLERDEMY GYVNIARNLD GFQQINLEEL MRSGDYAQIT 750
    NMLLNNQPVA LVGALPFITD SSVISLIAKL DATVFAQIVK LRKVDTLKPI 800
    LYKINSDSND FYLVANYDWI PTSTTKVYKQ VPQQFDFRAS MHMLTSNLTF 850
    TVYSDLLAFV SADTVEPINA VAFDNMRIMN EL 882
    Length:882
    Mass (Da):102,741
    Last modified:January 15, 2008 - v1
    Checksum:iD2D7006501643EC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ838635 Genomic RNA. Translation: ABG75814.1.
    RefSeqiYP_002302226.1. NC_011506.2.

    Genome annotation databases

    GeneIDi7011366.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ838635 Genomic RNA. Translation: ABG75814.1 .
    RefSeqi YP_002302226.1. NC_011506.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7011366.

    Family and domain databases

    InterProi IPR007779. Rotavirus_VP2.
    [Graphical view ]
    Pfami PF05087. Rota_VP2. 1 hit.
    [Graphical view ]
    ProDomi PD008866. Rota_VP2. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome heterogeneity of SA11 rotavirus due to reassortment with 'O' agent."
      Small C., Barro M., Brown T.L., Patton J.T.
      Virology 359:415-424(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Rotavirus RNA polymerase requires the core shell protein to synthesize the double-stranded RNA genome."
      Patton J.T., Jones M.T., Kalbach A.N., He Y.-W., Xiaobo J.
      J. Virol. 71:9618-9626(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING.
    3. "Mechanism for coordinated RNA packaging and genome replication by rotavirus polymerase VP1."
      Lu X., McDonald S.M., Tortorici M.A., Tao Y.J., Vasquez-Del Carpio R., Nibert M.L., Patton J.T., Harrison S.C.
      Structure 16:1678-1688(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiVP2_ROTSH
    AccessioniPrimary (citable) accession number: A2T3R5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 24 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3