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A2T3R5

- VP2_ROTSH

UniProt

A2T3R5 - VP2_ROTSH

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Protein

Inner capsid protein VP2

Gene
N/A
Organism
Rotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inner capsid protein VP2
OrganismiRotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58))
Taxonomic identifieri450149 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiCercopithecus pygerythrus (Vervet monkey) [TaxID: 60710]
ProteomesiUP000001119: Genome

Subcellular locationi

Virion Curated
Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

GO - Cellular componenti

  1. viral inner capsid Source: UniProtKB-KW
  2. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Inner capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882Inner capsid protein VP2PRO_0000367813Add
BLAST

Interactioni

Subunit structurei

Dimer (Potential). Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 (By similarity).By similarityCurated

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9494Interaction with VP1 and VP3By similarityAdd
BLAST
Regioni396 – 41621HydrophobicAdd
BLAST
Regioni424 – 44421HydrophobicAdd
BLAST
Regioni538 – 55922Leucine-zipper 1Add
BLAST
Regioni667 – 68822Leucine-zipper 2Add
BLAST

Domaini

The N-terminus is involved in VP1 binding.By similarity

Sequence similaritiesi

Belongs to the rotavirus VP2 family.Curated

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR007779. Rotavirus_VP2.
[Graphical view]
PfamiPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomiPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

A2T3R5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYRKRGARR ETNLKQDERM QEKEDSKNIN NDSPKSQLSE KVLSKKEEII
60 70 80 90 100
TDNQEEVKIS DEVKKSNKEE SKQLLEVLKT KEEHQKEVQY EILQKTIPTF
110 120 130 140 150
EPKESILKKL EDIKPEQAKK QTKLFRIFEP KQLPIYRANG ERELRNRWYW
160 170 180 190 200
KLKRDTLPDG DYDVREYFLN LYDQVLMEMP DYLLLKDMAV ENKNSRDAGK
210 220 230 240 250
VVDSETAAIC DAIFQDEETE GAVRRFIAEM RQRVQADRNV VNYPSILHPI
260 270 280 290 300
DHAFNEYFLQ HQLVEPLNND IIFNYIPERI RNDVNYILNM DRNLPSTARY
310 320 330 340 350
IRPNLLQDRL NLHDNFESLW DTITTSNYIL ARSVVPDLKE LVSTEAQIQK
360 370 380 390 400
MSQDLQLEAL TIQSETQFLT GINSQAANDC FKTLIAAMLS QRTMSLDFVT
410 420 430 440 450
TNYMSLISGM WLLTVIPNDM FIRESLVACQ LAIINTIVYP AFGMQRMHYR
460 470 480 490 500
NGDPQTPFQI AEQQIQNFQV ANWLHFVNYN QFRQVVIDGV LNQVLNDNIR
510 520 530 540 550
NGHVVNQLME ALMQLSRQQF PTMPVDYKRS IQRGILLLSN RLGQLVDLTR
560 570 580 590 600
LLSYNYETLM ACITMNMQHV QTLTTEKLQL TSVTSLCMLI GNATVIPSPQ
610 620 630 640 650
TLFHYYNVNV NFHSNYNERI NDAVAIITAA NRLNLYQKKM KSIVEDFLKR
660 670 680 690 700
LQIFDVARVP DDQMYRLRDR LRLLPVEIRR LDIFNLIAMN MEQIERASDK
710 720 730 740 750
IAQGVIIAYR DMQLERDEMY GYVNIARNLD GFQQINLEEL MRSGDYAQIT
760 770 780 790 800
NMLLNNQPVA LVGALPFITD SSVISLIAKL DATVFAQIVK LRKVDTLKPI
810 820 830 840 850
LYKINSDSND FYLVANYDWI PTSTTKVYKQ VPQQFDFRAS MHMLTSNLTF
860 870 880
TVYSDLLAFV SADTVEPINA VAFDNMRIMN EL
Length:882
Mass (Da):102,741
Last modified:January 15, 2008 - v1
Checksum:iD2D7006501643EC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ838635 Genomic RNA. Translation: ABG75814.1.
RefSeqiYP_002302226.1. NC_011506.2.

Genome annotation databases

GeneIDi7011366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ838635 Genomic RNA. Translation: ABG75814.1 .
RefSeqi YP_002302226.1. NC_011506.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7011366.

Family and domain databases

InterProi IPR007779. Rotavirus_VP2.
[Graphical view ]
Pfami PF05087. Rota_VP2. 1 hit.
[Graphical view ]
ProDomi PD008866. Rota_VP2. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Genome heterogeneity of SA11 rotavirus due to reassortment with 'O' agent."
    Small C., Barro M., Brown T.L., Patton J.T.
    Virology 359:415-424(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Rotavirus RNA polymerase requires the core shell protein to synthesize the double-stranded RNA genome."
    Patton J.T., Jones M.T., Kalbach A.N., He Y.-W., Xiaobo J.
    J. Virol. 71:9618-9626(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
  3. "Mechanism for coordinated RNA packaging and genome replication by rotavirus polymerase VP1."
    Lu X., McDonald S.M., Tortorici M.A., Tao Y.J., Vasquez-Del Carpio R., Nibert M.L., Patton J.T., Harrison S.C.
    Structure 16:1678-1688(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVP2_ROTSH
AccessioniPrimary (citable) accession number: A2T3R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: October 1, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3