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A2T3N5 (NSP3_ROTSH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-structural protein 3

Short name=NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name=NS34
OrganismRotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58)) [Reference proteome]
Taxonomic identifier450149 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostCercopithecus pygerythrus (Vervet monkey) [TaxID: 60710]

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

Subunit structure

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Sequence similarities

Belongs to the rotavirus A NSP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Non-structural protein 3
PRO_0000369445

Regions

Region4 – 149146RNA-binding By similarity
Region150 – 20657Dimerization By similarity
Region170 – 23465Interaction with ZC3H7B By similarity
Region208 – 315108Interaction with EIF4G1 By similarity
Coiled coil166 – 23772 Potential

Sequences

Sequence LengthMass (Da)Tools
A2T3N5 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 71029C07B95D4D21

FASTA31536,425
        10         20         30         40         50         60 
MLKMESTQQM AVSIINSSFE AAVVAATSAL ENMGIEYDYQ DIYSRVKNKF DFVMDDSGVK 

        70         80         90        100        110        120 
NNLIGKAITI DQALNNKFGS AIRNRNWLAD TSRAAKLDED VNKLRMMLSS KGIDQKMRVL 

       130        140        150        160        170        180 
NACFSVKRIP GKSSSIIKCT KLMRDKLERG EVEVDDSFVD EKMEVDTIDW KSRYEQLEQR 

       190        200        210        220        230        240 
FESLKSRVNE KYNNWVLKAR KMNENMHSLQ NVISQQQAHI AELQVYNNKL ERDLQNKIGS 

       250        260        270        280        290        300 
LTSSIEWYLR SMELDPEIKA DIEQQINSID AINPLHAFDD LESVIRNLIS DYDKLFLMFK 

       310 
GLIQRCNYQY SFGCE 

« Hide

References

[1]"Genome heterogeneity of SA11 rotavirus due to reassortment with 'O' agent."
Small C., Barro M., Brown T.L., Patton J.T.
Virology 359:415-424(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ838610 Genomic RNA. Translation: ABG75784.1.
RefSeqYP_002302220.1. NC_011501.2.

3D structure databases

ProteinModelPortalA2T3N5.
SMRA2T3N5. Positions 7-163, 206-315.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7011355.

Family and domain databases

InterProIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMSSF69903. SSF69903. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP3_ROTSH
AccessionPrimary (citable) accession number: A2T3N5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: July 9, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families