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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of translation Source: UniProtKB-KW
  2. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (isolate Monkey/South Africa/SA11-H96/1958 G3-P5B[2]-I2-R2-C5-M5-A5-N5-T5-E2-H5) (RV-A) (Simian Agent 11 (isolate SI/South Africa/H96/58))
Taxonomic identifieri450149 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiCercopithecus pygerythrus (Vervet monkey) [TaxID: 60710]
ProteomesiUP000001119: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Non-structural protein 3PRO_0000369445Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliA2T3N5.
SMRiA2T3N5. Positions 7-163, 206-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-bindingBy similarityAdd
BLAST
Regioni150 – 20657DimerizationBy similarityAdd
BLAST
Regioni170 – 23465Interaction with ZC3H7BBy similarityAdd
BLAST
Regioni208 – 315108Interaction with EIF4G1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

A2T3N5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKMESTQQM AVSIINSSFE AAVVAATSAL ENMGIEYDYQ DIYSRVKNKF
60 70 80 90 100
DFVMDDSGVK NNLIGKAITI DQALNNKFGS AIRNRNWLAD TSRAAKLDED
110 120 130 140 150
VNKLRMMLSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT KLMRDKLERG
160 170 180 190 200
EVEVDDSFVD EKMEVDTIDW KSRYEQLEQR FESLKSRVNE KYNNWVLKAR
210 220 230 240 250
KMNENMHSLQ NVISQQQAHI AELQVYNNKL ERDLQNKIGS LTSSIEWYLR
260 270 280 290 300
SMELDPEIKA DIEQQINSID AINPLHAFDD LESVIRNLIS DYDKLFLMFK
310
GLIQRCNYQY SFGCE
Length:315
Mass (Da):36,425
Last modified:January 15, 2008 - v1
Checksum:i71029C07B95D4D21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ838610 Genomic RNA. Translation: ABG75784.1.
RefSeqiYP_002302220.1. NC_011501.2.

Genome annotation databases

GeneIDi7011355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ838610 Genomic RNA. Translation: ABG75784.1.
RefSeqiYP_002302220.1. NC_011501.2.

3D structure databases

ProteinModelPortaliA2T3N5.
SMRiA2T3N5. Positions 7-163, 206-315.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7011355.

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome heterogeneity of SA11 rotavirus due to reassortment with 'O' agent."
    Small C., Barro M., Brown T.L., Patton J.T.
    Virology 359:415-424(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP3_ROTSH
AccessioniPrimary (citable) accession number: A2T3N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: January 7, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.