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A2T3M2

- NSP2_ROTAP

UniProt

A2T3M2 - NSP2_ROTAP

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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (isolate Cow/South Africa/Offal agent/1965 G8-P6[1]-Ix-Rx-Cx-Mx-A11-N2-Tx-Ex-Hx) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Magnesium is required for NTPase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531MagnesiumSequence Analysis
Metal bindingi171 – 1711MagnesiumSequence Analysis
Active sitei225 – 2251For NTPase activityBy similarity

GO - Molecular functioni

  1. hydrolase activity, acting on acid anhydrides Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus A (isolate Cow/South Africa/Offal agent/1965 G8-P6[1]-Ix-Rx-Cx-Mx-A11-N2-Tx-Ex-Hx) (RV-A)
Taxonomic identifieri578837 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

Host cytoplasm Curated
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Non-structural protein 2PRO_0000369528Add
BLAST

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliA2T3M2.
SMRiA2T3M2. Positions 1-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 24137RNA-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

A2T3M2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELACFCYP HLENDSYRFI PFNSLAIKCM LTAKVDKKDQ DKFYNSIIYG
60 70 80 90 100
IAPPPQFKKR YNTSDNSRGM NYETSMFNKV AALICEALNS IKVTQSDVAS
110 120 130 140 150
VLSKIVSVRH LENLVLRREN HQDVLFHSKE LLLKSVLIAI GHSKEIETTA
160 170 180 190 200
TAEGGEIVFQ NAAFTMWKLT YLEHKLMPIL DQNFIEYKIT LNEDKPISES
210 220 230 240 250
HVKELIAELR WQYNKFAVIT HGKGHYRVVK YSSVANHADR VYATFKSNNK
260 270 280 290 300
NGNMIEFNLL DQRIIWQNWY AFTSSMKQGN TLEICKKLLF QKMKRESNPF
310
KGLSTDRKMD EVSQIGI
Length:317
Mass (Da):36,628
Last modified:March 6, 2007 - v1
Checksum:i25670C29E6607474
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ838597 Genomic RNA. Translation: ABG75771.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ838597 Genomic RNA. Translation: ABG75771.1 .

3D structure databases

ProteinModelPortali A2T3M2.
SMRi A2T3M2. Positions 1-313.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProi IPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view ]
Pfami PF02509. Rota_NS35. 1 hit.
[Graphical view ]
SUPFAMi SSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome heterogeneity of SA11 rotavirus due to reassortment with 'O' agent."
    Small C., Barro M., Brown T.L., Patton J.T.
    Virology 359:415-424(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP2_ROTAP
AccessioniPrimary (citable) accession number: A2T3M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 6, 2007
Last modified: November 26, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3