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A2T3M2 (NSP2_ROTAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 2

Short name=NSP2
EC=3.6.4.-
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name=NS35
OrganismRotavirus A (isolate Cow/South Africa/Offal agent/1965 G8-P6[1]-Ix-Rx-Cx-Mx-A11-N2-Tx-Ex-Hx) (RV-A)
Taxonomic identifier578837 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.

Cofactor

Magnesium for NTPase activity By similarity.

Subunit structure

Homooctamer By similarity. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories By similarity.

Subcellular location

Host cytoplasm Potential. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the rotavirus NSP2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Non-structural protein 2
PRO_0000369528

Regions

Region205 – 24137RNA-binding Potential

Sites

Active site2251For NTPase activity By similarity
Metal binding1531Magnesium Potential
Metal binding1711Magnesium Potential

Sequences

Sequence LengthMass (Da)Tools
A2T3M2 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 25670C29E6607474

FASTA31736,628
        10         20         30         40         50         60 
MAELACFCYP HLENDSYRFI PFNSLAIKCM LTAKVDKKDQ DKFYNSIIYG IAPPPQFKKR 

        70         80         90        100        110        120 
YNTSDNSRGM NYETSMFNKV AALICEALNS IKVTQSDVAS VLSKIVSVRH LENLVLRREN 

       130        140        150        160        170        180 
HQDVLFHSKE LLLKSVLIAI GHSKEIETTA TAEGGEIVFQ NAAFTMWKLT YLEHKLMPIL 

       190        200        210        220        230        240 
DQNFIEYKIT LNEDKPISES HVKELIAELR WQYNKFAVIT HGKGHYRVVK YSSVANHADR 

       250        260        270        280        290        300 
VYATFKSNNK NGNMIEFNLL DQRIIWQNWY AFTSSMKQGN TLEICKKLLF QKMKRESNPF 

       310 
KGLSTDRKMD EVSQIGI 

« Hide

References

[1]"Genome heterogeneity of SA11 rotavirus due to reassortment with 'O' agent."
Small C., Barro M., Brown T.L., Patton J.T.
Virology 359:415-424(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ838597 Genomic RNA. Translation: ABG75771.1.

3D structure databases

ProteinModelPortalA2T3M2.
SMRA2T3M2. Positions 1-313.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP2_ROTAP
AccessionPrimary (citable) accession number: A2T3M2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 6, 2007
Last modified: April 16, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families