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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

tapD

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the TapA pilin precursor during membrane translocation (By similarity).By similarity

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Protease, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciASAL382245:GJJN-408-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:tapD
Synonyms:pilD
Ordered Locus Names:ASA_0411
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence analysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence analysisAdd
BLAST
Transmembranei162 – 18221HelicalSequence analysisAdd
BLAST
Transmembranei186 – 20621HelicalSequence analysisAdd
BLAST
Transmembranei232 – 25221HelicalSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Type 4 prepilin-like proteins leader peptide-processing enzymePRO_0000289862Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi382245.ASA_0411.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
HOGENOMiHOG000248584.
KOiK02654.
OMAiVFWLFKL.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

A2T195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLLELAHG LPWLYFSLVF LFSLMIGSFL NVVIHRLPIM LEREWQAEYR
60 70 80 90 100
SYFSSDTPQP EDDERYNLMV PRSCCPRCNH PITALENIPL LSWLWLKGRC
110 120 130 140 150
RGCQAAISAR YPLVELLTAL LSVVVAMTLT PGWGTLAALL LTWVLVALTF
160 170 180 190 200
IDLDKMLLPD QLTLPLLWGG LLFNLLGGYV PLGDAVIGAM AGYLVLWSLY
210 220 230 240 250
WAFKLLTGKE GMGYGDFKLL AALGAWLGWQ ALPIVLLLSS LVGAIFGIGL
260 270 280 290
ILLRNHHQSK PIPFGPYLAI AGWIALLWGD SITRWYLSTI L
Length:291
Mass (Da):32,501
Last modified:March 6, 2007 - v1
Checksum:i4CFFA0AFC886E368
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601P → A in AAC04561 (Ref. 1) Curated
Sequence conflicti84 – 841A → G in AAC04561 (Ref. 1) Curated
Sequence conflicti100 – 1001C → W in AAC04561 (Ref. 1) Curated
Sequence conflicti103 – 1031C → W in AAC04561 (Ref. 1) Curated
Sequence conflicti126 – 1261A → G in AAC04561 (Ref. 1) Curated
Sequence conflicti163 – 1631T → N in AAC04561 (Ref. 1) Curated
Sequence conflicti198 – 1981S → R in AAC04561 (Ref. 1) Curated
Sequence conflicti253 – 2531L → V in AAC04561 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95640 Genomic DNA. Translation: AAC04561.1.
DQ396478 Genomic DNA. Translation: ABD57323.1.
CP000644 Genomic DNA. Translation: ABO88591.1.
RefSeqiWP_005314162.1. NC_009348.1.

Genome annotation databases

EnsemblBacteriaiABO88591; ABO88591; ASA_0411.
GeneIDi4995523.
KEGGiasa:ASA_0411.
PATRICi20787124. VBIAerSal2987_0417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95640 Genomic DNA. Translation: AAC04561.1.
DQ396478 Genomic DNA. Translation: ABD57323.1.
CP000644 Genomic DNA. Translation: ABO88591.1.
RefSeqiWP_005314162.1. NC_009348.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi382245.ASA_0411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO88591; ABO88591; ASA_0411.
GeneIDi4995523.
KEGGiasa:ASA_0411.
PATRICi20787124. VBIAerSal2987_0417.

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
HOGENOMiHOG000248584.
KOiK02654.
OMAiVFWLFKL.

Enzyme and pathway databases

BioCyciASAL382245:GJJN-408-MONOMER.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Entry informationi

Entry nameiLEP4_AERS4
AccessioniPrimary (citable) accession number: A2T195
Secondary accession number(s): O54483, O68964
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 6, 2007
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.