A2SXS5 (MEN1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Menin

Gene names

Name:

MEN1

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair By similarity.
Subunit structure	Component of the MLL-HCF complex, at least composed of MLL, MEN1, ASH2L, RBBP5, DPY30, WDR5, HCFC1 and HCFC2 By similarity. Component of the menin-associated histone methyltransferase complex, at least composed of MLL4/WBP7, MEN1, ASH2L, RBBP5, DPY30 and WDR5 By similarity. Interacts with POLR2B By similarity. Interacts with POLR2A phosphorylated at 'Ser-5', but not with the unphosphorylated, nor 'Ser-2' phosphorylated POLR2A forms By similarity. Interacts with FANCD2 and DBF4 By similarity. Interacts with SMAD3, but not with SMAD2, nor SMAD4 By similarity. Directly interacts with NFKB1, NFKB2 and RELA. Interacts with JUND By similarity.
Subcellular location	Nucleus By similarity.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: Compara cell cycle arrest Inferred from electronic annotation. Source: Compara chromatin remodeling Inferred from electronic annotation. Source: Compara embryonic skeletal system morphogenesis Inferred from electronic annotation. Source: Compara hemopoiesis Inferred from electronic annotation. Source: Compara leukocyte homeostasis Inferred from electronic annotation. Source: Compara maternal process involved in female pregnancy Inferred from electronic annotation. Source: Compara negative regulation of JNK cascade Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: Compara negative regulation of organ growth Inferred from electronic annotation. Source: Compara negative regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara negative regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara negative regulation of telomerase activity Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara osteoblast development Inferred from electronic annotation. Source: Compara osteoblast fate commitment Inferred from electronic annotation. Source: Compara palate development Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of cell division Inferred from electronic annotation. Source: Compara positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of histone methylation Inferred from electronic annotation. Source: Compara positive regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara positive regulation of protein binding Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara response to DNA damage stimulus Inferred from electronic annotation. Source: Compara response to UV Inferred from electronic annotation. Source: Compara response to gamma radiation Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chromatin Inferred from electronic annotation. Source: Compara cleavage furrow Inferred from electronic annotation. Source: Compara cytosol Inferred from electronic annotation. Source: Compara histone methyltransferase complex Inferred from electronic annotation. Source: Compara nuclear matrix Inferred from electronic annotation. Source: Compara
Molecular_function	Y-form DNA binding Inferred from electronic annotation. Source: Compara chromatin binding Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from electronic annotation. Source: Compara four-way junction DNA binding Inferred from electronic annotation. Source: Compara histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from electronic annotation. Source: Compara transcription regulatory region DNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A2SXS5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A2SXS5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     150-154: Missing.

Note: Gene prediction based on similarity to human ortholog.

Isoform 3 (identifier: A2SXS5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     401-455: Missing.

Note: Gene prediction based on similarity to mouse ortholog.

Isoform 4 (identifier: A2SXS5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     150-154: Missing.
     401-455: Missing.

Note: Gene prediction.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Menin		PRO_0000408472
Regions
	Region	219 – 395	177	Interaction with FANCD2 By similarity
Amino acid modifications
	Modified residue	599	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	150 – 154	5	Missing in isoform 2 and isoform 4.		VSP_041101
	Alternative sequence	401 – 455	55	Missing in isoform 3 and isoform 4.		VSP_041102

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 52FB0491302AC165	FASTA	615	67,962
	10 20 30 40 50 60 MGLKAAQKTL FPLRSIDDVV RLFAAELGRE EPDLVLLSLV LGFVEHFLAV NRVIPTNVPE 70 80 90 100 110 120 LTFQPSPAPD PPGGLTYFPV ADLSIIAALY ARFTAQIRGA VDLSLYPREG GVSSRELVKK 130 140 150 160 170 180 VSDVIWNSLS RSYFKDRAHI QSLFSFITGW SPTGTKLDSS GVAFAVVGAC QALGLRDVHL 190 200 210 220 230 240 ALSEDHAWVV FGPNGEQTAE VTWHGKGNED RRGQTVNAGV AERSWLYLKG SYMRCDRKME 250 260 270 280 290 300 VAFMVCAINP SIDLHTDSLE LLQLQQKLLW LLYDLGHLER YPMALGNLAD LEELEPTPGR 310 320 330 340 350 360 PDPLTLYHKG IASAKTYYRD EHIYPYMYLA GYHCRNRNVR EALQAWADTA TVIQDYNYCR 370 380 390 400 410 420 EDEEIYKEFF EVANDVIPNL LKEAASLLEA GEERPGEQTQ GVQSQGSALQ DPECFAHLLR 430 440 450 460 470 480 FYDGICKWEE GSPTPVLHVG WATFLVQSLG RFEGQVRQKV RIVSREAEAA EAEELWGEEA 490 500 510 520 530 540 REGRRRGPRR ESKPEEPPPP KKPALDKGPG GGQGAMSGPP RKPPGTVPGT ARGPEGGSTA 550 560 570 580 590 600 PAPAPAASPP PEGPVLTFQS EKMKGMKELL VATKINSSAI KLQLTAQSQV QMKKQKVSTP 610 SDYTLSFLKR QRKGL
	Isoform 2 [UniParc]. Checksum: 071D4C293176D637	FASTA	610	67,434
	10 20 30 40 50 60 MGLKAAQKTL FPLRSIDDVV RLFAAELGRE EPDLVLLSLV LGFVEHFLAV NRVIPTNVPE 70 80 90 100 110 120 LTFQPSPAPD PPGGLTYFPV ADLSIIAALY ARFTAQIRGA VDLSLYPREG GVSSRELVKK 130 140 150 160 170 180 VSDVIWNSLS RSYFKDRAHI QSLFSFITGT KLDSSGVAFA VVGACQALGL RDVHLALSED 190 200 210 220 230 240 HAWVVFGPNG EQTAEVTWHG KGNEDRRGQT VNAGVAERSW LYLKGSYMRC DRKMEVAFMV 250 260 270 280 290 300 CAINPSIDLH TDSLELLQLQ QKLLWLLYDL GHLERYPMAL GNLADLEELE PTPGRPDPLT 310 320 330 340 350 360 LYHKGIASAK TYYRDEHIYP YMYLAGYHCR NRNVREALQA WADTATVIQD YNYCREDEEI 370 380 390 400 410 420 YKEFFEVAND VIPNLLKEAA SLLEAGEERP GEQTQGVQSQ GSALQDPECF AHLLRFYDGI 430 440 450 460 470 480 CKWEEGSPTP VLHVGWATFL VQSLGRFEGQ VRQKVRIVSR EAEAAEAEEL WGEEAREGRR 490 500 510 520 530 540 RGPRRESKPE EPPPPKKPAL DKGPGGGQGA MSGPPRKPPG TVPGTARGPE GGSTAPAPAP 550 560 570 580 590 600 AASPPPEGPV LTFQSEKMKG MKELLVATKI NSSAIKLQLT AQSQVQMKKQ KVSTPSDYTL 610 SFLKRQRKGL
	Isoform 3 [UniParc]. Checksum: E97DA5DAFE831AB9	FASTA	560	61,887
	10 20 30 40 50 60 MGLKAAQKTL FPLRSIDDVV RLFAAELGRE EPDLVLLSLV LGFVEHFLAV NRVIPTNVPE 70 80 90 100 110 120 LTFQPSPAPD PPGGLTYFPV ADLSIIAALY ARFTAQIRGA VDLSLYPREG GVSSRELVKK 130 140 150 160 170 180 VSDVIWNSLS RSYFKDRAHI QSLFSFITGW SPTGTKLDSS GVAFAVVGAC QALGLRDVHL 190 200 210 220 230 240 ALSEDHAWVV FGPNGEQTAE VTWHGKGNED RRGQTVNAGV AERSWLYLKG SYMRCDRKME 250 260 270 280 290 300 VAFMVCAINP SIDLHTDSLE LLQLQQKLLW LLYDLGHLER YPMALGNLAD LEELEPTPGR 310 320 330 340 350 360 PDPLTLYHKG IASAKTYYRD EHIYPYMYLA GYHCRNRNVR EALQAWADTA TVIQDYNYCR 370 380 390 400 410 420 EDEEIYKEFF EVANDVIPNL LKEAASLLEA GEERPGEQTQ VRQKVRIVSR EAEAAEAEEL 430 440 450 460 470 480 WGEEAREGRR RGPRRESKPE EPPPPKKPAL DKGPGGGQGA MSGPPRKPPG TVPGTARGPE 490 500 510 520 530 540 GGSTAPAPAP AASPPPEGPV LTFQSEKMKG MKELLVATKI NSSAIKLQLT AQSQVQMKKQ 550 560 KVSTPSDYTL SFLKRQRKGL
	Isoform 4 [UniParc]. Checksum: 7D58AA09F422AB8E	FASTA	555	61,358
	10 20 30 40 50 60 MGLKAAQKTL FPLRSIDDVV RLFAAELGRE EPDLVLLSLV LGFVEHFLAV NRVIPTNVPE 70 80 90 100 110 120 LTFQPSPAPD PPGGLTYFPV ADLSIIAALY ARFTAQIRGA VDLSLYPREG GVSSRELVKK 130 140 150 160 170 180 VSDVIWNSLS RSYFKDRAHI QSLFSFITGT KLDSSGVAFA VVGACQALGL RDVHLALSED 190 200 210 220 230 240 HAWVVFGPNG EQTAEVTWHG KGNEDRRGQT VNAGVAERSW LYLKGSYMRC DRKMEVAFMV 250 260 270 280 290 300 CAINPSIDLH TDSLELLQLQ QKLLWLLYDL GHLERYPMAL GNLADLEELE PTPGRPDPLT 310 320 330 340 350 360 LYHKGIASAK TYYRDEHIYP YMYLAGYHCR NRNVREALQA WADTATVIQD YNYCREDEEI 370 380 390 400 410 420 YKEFFEVAND VIPNLLKEAA SLLEAGEERP GEQTQVRQKV RIVSREAEAA EAEELWGEEA 430 440 450 460 470 480 REGRRRGPRR ESKPEEPPPP KKPALDKGPG GGQGAMSGPP RKPPGTVPGT ARGPEGGSTA 490 500 510 520 530 540 PAPAPAASPP PEGPVLTFQS EKMKGMKELL VATKINSSAI KLQLTAQSQV QMKKQKVSTP 550 SDYTLSFLKR QRKGL

References

[1]

"Inheritance, mode of inheritance, and candidate genes for primary hyperparathyroidism in Keeshonden." Goldstein R.E., Atwater D.Z., Cazolli D.M., Goldstein O., Wade C.M., Lindblad-Toh K. J. Vet. Intern. Med. 21:199-203(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ366289 mRNA. Translation: ABD16380.1.
RefSeq	NP_001074977.1. NM_001081508.1.
UniGene	Cfa.21138.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000020909.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000022520; ENSCAFP00000020909; ENSCAFG00000014185. ENSCAFT00000022521; ENSCAFP00000020910; ENSCAFG00000014185.
GeneID	483758.
KEGG	cfa:483758.
Organism-specific databases
CTD	4221.
Phylogenomic databases
eggNOG	NOG67412.
GeneTree	ENSGT00390000014237.
HOGENOM	HOG000007225.
HOVERGEN	HBG000208.
InParanoid	A2SXS5.
KO	K14970.
OMA	NNEHIYP.
OrthoDB	EOG4HT8RX.
Family and domain databases
InterPro	IPR007747. Menin. [Graphical view]
PANTHER	PTHR12693. PTHR12693. 1 hit.
Pfam	PF05053. Menin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20858110.

Entry information

Entry name

MEN1_CANFA

Accession

Primary (citable) accession number: A2SXS5
Secondary accession number(s): E2RDP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program