Reviewed, UniProtKB/Swiss-Prot A2SW69 (ANXA2_SHEEP)

Names and origin

Protein names

Recommended name:
    Annexin A2
Alternative name(s):
    Annexin-2

Gene names

Name:	ANXA2
Synonyms:	ANX2

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity By similarity.
Subunit structure	Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Note: In the lamina beneath the plasma membrane By similarity.
Tissue specificity	Detected in myometrium. Ref.1
Induction	Up-regulated in myometrium upon progesterone treatment. Ref.1
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Miscellaneous	It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Cellular component	basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW cytoskeletal protein binding Inferred from electronic annotation. Source: InterPro phospholipase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 339	338	Annexin A2		PRO_0000288687
Regions
	Repeat	42 – 102	61	Annexin 1
	Repeat	114 – 174	61	Annexin 2
	Repeat	199 – 259	61	Annexin 3
	Repeat	274 – 334	61	Annexin 4
	Region	2 – 24	23	S100A10-binding site Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	18	1	Phosphoserine By similarity
	Modified residue	19	1	Phosphothreonine By similarity
	Modified residue	24	1	Phosphotyrosine; by SRC By similarity
	Modified residue	26	1	Phosphoserine; by PKC By similarity
	Modified residue	30	1	Phosphotyrosine By similarity
	Modified residue	104	1	N6-acetyllysine By similarity
	Modified residue	115	1	N6-acetyllysine By similarity
	Modified residue	148	1	N6-acetyllysine By similarity
	Modified residue	152	1	N6-acetyllysine By similarity
	Modified residue	157	1	N6-acetyllysine By similarity
	Modified residue	188	1	Phosphotyrosine By similarity
	Modified residue	199	1	Phosphotyrosine By similarity
	Modified residue	227	1	N6-acetyllysine By similarity
	Modified residue	238	1	Phosphotyrosine By similarity
	Modified residue	275	1	Phosphotyrosine By similarity
	Modified residue	279	1	N6-acetyllysine By similarity
	Modified residue	302	1	N6-acetyllysine By similarity
	Modified residue	313	1	N6-acetyllysine By similarity
	Modified residue	316	1	Phosphotyrosine By similarity
	Modified residue	317	1	Phosphotyrosine By similarity
	Modified residue	318	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SW69-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: AA8E2500F4138B3D	FASTA	339	38,612
	10 20 30 40 50 60 MSTVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG VDEVTIVNIL 70 80 90 100 110 120 TNRSNEQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG 130 140 150 160 170 180 LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDIV SDTSGDFRKL MVALAKGRRA 190 200 210 220 230 240 EDGSVIDYEL IDQDARDLYD AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM 250 260 270 280 290 300 LESIKKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM 310 320 330 LKIRSEFKKK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD

References

[1]

"Separate and synergistic effect of progesterone and estradiol on induction of annexin 2 and its interaction protein p11 in pregnant sheep myometrium." Zhang Q., Wu W.X. J. Mol. Endocrinol. 38:441-454(2007) [PubMed: 17446234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY, SUBUNIT. Tissue: Myometrium.

Web resources

Protein Spotlight

Red velvet - Issue 86 of September 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ358998 mRNA. Translation: ABC94470.1.
RefSeq	NP_001087257.1.
UniGene	Oar.5185
3D structure databases
SMR	A2SW69. Positions 21-339.
ModBase	Search...
Genome annotation databases
GeneID	100048993.
Organism-specific databases
CTD	100048993.
Phylogenomic databases
HOVERGEN	A2SW69.
Family and domain databases
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR002389. AnnexinII. [Graphical view]
Gene3D	G3DSA:1.10.220.10. Annexin. 4 hits.
PANTHER	PTHR10502. Annexin. 1 hit. PTHR10502:SF18. AnnexinII. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00198. ANNEXINII.
SMART	SM00335. ANX. 4 hits. [Graphical view]
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANXA2_SHEEP

Accession

Primary (citable) accession number: A2SW69

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 6, 2007
Last modified:	November 24, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)