Reviewed, UniProtKB/Swiss-Prot A2SU54 (DP2L_METLZ)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	Mlab_1699

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1141	1141	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294687

Sequences

	Sequence		Length	Mass (Da)
	A2SU54-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: E52E99960EC631DD	FASTA	1,141	126,420
	10 20 30 40 50 60 MAELVTSPAY RAYLDGLIAG LDRAMEIANK AKSLGVDPRP YVEIPIASDL AGRVEALLGY 70 80 90 100 110 120 KGVAAKIREL EDRMSREEAA LKIGDAFVGK EFGESTREDI LDHAIRTSMA LLTEGVVAAP 130 140 150 160 170 180 TEGIGKVAVR RNDDGTEYLS IYYAGPIRSA GGTAQALSVL VGDYVRRLLN IDRYKPREEE 190 200 210 220 230 240 IERYVEEIKQ YNNIQSMQYL PKDDDIRLII RNCPICIDGE PTEQEEISGY RNLERVETNV 250 260 270 280 290 300 VRGGMGLVIA EGIGLKAPKI QKNVAKMNLD GWEWLEELIS GNTPAQEVEE EPGVHPKDKY 310 320 330 340 350 360 MRDMLAGRPS FSYPMRKGGF RFRLGRCRNT GLATCGFNPA TLHILDDYLA VGTQMKVERP 370 380 390 400 410 420 GKACGVVPCT DIEGPTVRLK SGELRRIDTL DDANKYYDQV EYILDIGEIL ISFGEFMENN 430 440 450 460 470 480 HVLMPPSYCE EWWIQEGGPR HPKNEAEALS FVLEGAYLHP DYTWFWDDCS EGQLIFLSDK 490 500 510 520 530 540 VAGTGSLREG VLYIPEDPAV KSVLEELLVP HTVEEGFYVV RTPLALIMGL GLTDTLAKSP 550 560 570 580 590 600 TWKTLPPFSN GLSMAISLSG LKMRSKAGTR VGGRMGRPGK SAPRKMKPPV HVLFPIGESG 610 620 630 640 650 660 GMKRSIDNAA KICSSDSEEN FRGTSVTTGQ VEGLIQVQTG ERRCPKCGTV TFKSRCADCG 670 680 690 700 710 720 THTDAVYRCP HCNQLGEEGQ ESCPKCGANL VCSKESIVSL GQEYAAALKN VGMSASSSPE 730 740 750 760 770 780 LKGVRGLISR ERVVEPLEKG LLRAKNNIFV FRDGTIRYDM IDLPLTHFRP AEVGVSVEKL 790 800 810 820 830 840 RSIGYTQDMH GADLTDASQL VELHPQDIMV SVDCGEYLVR VAAYVDELLE KVYGMEAFYH 850 860 870 880 890 900 VKTPEDLVGH LVMGLAPHTS AGVLARIVGF TKAKAGYAHP YYHAAKRRNC DGDEDCVMLL 910 920 930 940 950 960 MDGLLNFSRS FLPSTRGGTM DAPLVLTTTL NPKEVDKETL NVDVMPRYPL EVYTACLTYR 970 980 990 1000 1010 1020 APKEVGKFVD YVEKRVETPG QFEGFSFTHD TTDISEGPID TMYTNPILKG TADKIKAELG 1030 1040 1050 1060 1070 1080 LADRIRAVDT NDLAERIINS HLMPDMIGNL RSFSKQAFRC PKCKTSYRRI PVSGKCNKCG 1090 1100 1110 1120 1130 1140 GPVKATMHKG NVTKYLEISK YMAEHYTLSD YTNQRIQVTE MNINSTFGEE EKVQMDLSDF F

References

[1]

"Complete sequence of Methanocorpusculum labreanum Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Whitman B., Olsen G., Woese C., Richardson P. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000559 Genomic DNA. Translation: ABN07860.1.
RefSeq	YP_001031127.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2SU54.
Genome annotation databases
GeneID	4795011.
GenomeReviews	Gene locus Mlab_1699 in contig CP000559_GR.
KEGG	mla:Mlab_1699.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NCDGDED.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METLZ

Accession

Primary (citable) accession number: A2SU54

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 6, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)