Reviewed, UniProtKB/Swiss-Prot A2STY4 (RIFK_METLZ)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Mlab_1629

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Riboflavin kinase HAMAP MF_01285		PRO_0000322092
Regions
	Nucleotide binding	104 – 109	6	CDP By similarity
	Nucleotide binding	203 – 206	4	CDP By similarity
	Region	1 – 94	94	H-T-H motif-like HAMAP MF_01285
	Region	95 – 222	128	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	133	1	Magnesium By similarity
	Metal binding	135	1	Magnesium By similarity
	Binding site	190	1	FMN By similarity
	Binding site	198	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2STY4-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 8766FBD0417E1459	FASTA	222	24,735
	10 20 30 40 50 60 METIDAEDAA CLRRIALLGG CKGPVRLSTQ ALGDQLGISQ QTASRRLQSL EKAQMISRTA 70 80 90 100 110 120 ESTGQYVLVT RSGEEHLRRE FAEYAKIFDV KDEQYVLTGT VMSGVGEGRY YMSIPHYQEQ 130 140 150 160 170 180 FEKLCGFTPY PGTLNIKLNP QSVLIRKRMD SLEWTIVPGF KDEHRMFGEA RCIKCTISGI 190 200 210 220 PCAIVVPGRT HHPEEVIEVI SGTQLRDALD LTENSEVVVV IG

References

[1]

"Complete sequence of Methanocorpusculum labreanum Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Whitman B., Olsen G., Woese C., Richardson P. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000559 Genomic DNA. Translation: ABN07790.1.
RefSeq	YP_001031057.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2STY4.
Genome annotation databases
GeneID	4795257.
GenomeReviews	Gene locus Mlab_1629 in contig CP000559_GR.
KEGG	mla:Mlab_1629.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GAYYVRQ.
Family and domain databases
HAMAP	MF_01285. Fused. [Tree]
InterPro	IPR001808. HTH_Crp. IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF00325. Crp. 1 hit. PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_METLZ

Accession

Primary (citable) accession number: A2STY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 6, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)