A2STY4 (RIFK_METLZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Mlab_1629

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Riboflavin kinase HAMAP MF_01285		PRO_0000322092
Regions
	Nucleotide binding	104 – 109	6	CDP By similarity
	Nucleotide binding	203 – 206	4	CDP By similarity
	Region	1 – 94	94	H-T-H motif-like HAMAP MF_01285
	Region	95 – 222	128	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	133	1	Magnesium By similarity
	Metal binding	135	1	Magnesium By similarity
	Binding site	190	1	FMN By similarity
	Binding site	198	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2STY4 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 8766FBD0417E1459	FASTA	222	24,735
	10 20 30 40 50 60 METIDAEDAA CLRRIALLGG CKGPVRLSTQ ALGDQLGISQ QTASRRLQSL EKAQMISRTA 70 80 90 100 110 120 ESTGQYVLVT RSGEEHLRRE FAEYAKIFDV KDEQYVLTGT VMSGVGEGRY YMSIPHYQEQ 130 140 150 160 170 180 FEKLCGFTPY PGTLNIKLNP QSVLIRKRMD SLEWTIVPGF KDEHRMFGEA RCIKCTISGI 190 200 210 220 PCAIVVPGRT HHPEEVIEVI SGTQLRDALD LTENSEVVVV IG

References

[1]

"Complete genome sequence of Methanocorpusculum labreanum type strain Z." Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:197-203(2009) [PubMed: 21304657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000559 Genomic DNA. Translation: ABN07790.1.
RefSeq	YP_001031057.1. NC_008942.1.
3D structure databases
ProteinModelPortal	A2STY4.
ModBase	Search...
Protein-protein interaction databases
STRING	A2STY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4795257.
GenomeReviews	Gene locus Mlab_1629 in contig CP000559_GR.
KEGG	mla:Mlab_1629.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK949639.
Enzyme and pathway databases
BioCyc	MLAB410358:MLAB_1629-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_METLZ

Accession

Primary (citable) accession number: A2STY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 6, 2007
Last modified:	November 16, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program