A2ST90 (SYA_METLZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Mlab_1380

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	913 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 913	913	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_1000074509
Sites
	Metal binding	608	1	Zinc By similarity
	Metal binding	612	1	Zinc By similarity
	Metal binding	711	1	Zinc By similarity
	Metal binding	715	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2ST90 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 9081336847C9BDA0	FASTA	913	101,779
	10 20 30 40 50 60 MLENEYQLEY FKENGFIRKI CKKCGSAFWT LDPNREICGD APCEPYQFIG NPIFTKHSLT 70 80 90 100 110 120 EMREAYLSFF ERHGHTRINR YPVAARWRDD IYLTIASIAD FQPFVTSGVC PPPANPLTIS 130 140 150 160 170 180 QPCIRLNDLD NVGRSGRHFT CFEMMAHHAF NTDEKPIYWK DRCLELCSGF IESLGGNVFD 190 200 210 220 230 240 LTYKENPWFG GGNAGPSVEV LMGGLEVATL VFMNLSRKNS GKPPVSIDGK DYYEMPLRIV 250 260 270 280 290 300 DTGYGLERFT WASCGTPTAY DAVFPEMIPR ILTAAGMEDR LENPEVERIL GLNAKFAGLM 310 320 330 340 350 360 DIRGEKIRDL RQQVADATNI SVAKLEEIIV PMETVYALCD HTRCLAYMLG DLIVPSNVRE 370 380 390 400 410 420 GYLARLVLRR SIRMMQDLNM DDDLGDLVVS QMKTIGLANF EQDEDIVRHI INREVEKYDT 430 440 450 460 470 480 TIERGTRTVQ RVGQTYVKKN EPVPLAELIT LYDSHGIPVD LMGKILKDTG AEFEIPDDFD 490 500 510 520 530 540 SQIADMHSEN ETEKPVSPLA KYAERIAKIP ETRKSFYERP ADMEFDATVL DIFDNYVVLD 550 560 570 580 590 600 ATLFYPEGGG QPSDTGMLVT KSTMVRVDEV VKWENVILHK VRENTLKRGD RVKGVLDEDR 610 620 630 640 650 660 RWALMRHHSA THMVLRAAKE VLGPHVHQAG SQLSTDVARL DIRHYTHITP EELKQMETIA 670 680 690 700 710 720 NRLVMENLPT MVKIENRVKA EQKFGFALYQ GGVPPGKDIR VVQMGAEVQA CAGTHCQSTG 730 740 750 760 770 780 EIGPIKILKL EHIQDGVERI EFAAGFAALD AMQHIQSLLN TSADTLSVQT ENLPGSVDRF 790 800 810 820 830 840 FTEWKDQRKE IEKLRAKIAE LELSRIEGIN IGGVEVVIKQ IDVSRKELVT VAGKIAERGG 850 860 870 880 890 900 VTVLITTADG LGVVASSGTG KIHAGKLVGE VCAELGGKGG GKENLAQGAG ADPSAVGKAL 910 LKAESFIRAE FNS

References

[1]

"Complete genome sequence of Methanocorpusculum labreanum type strain Z." Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:197-203(2009) [PubMed: 21304657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000559 Genomic DNA. Translation: ABN07546.1.
RefSeq	YP_001030813.1. NC_008942.1.
3D structure databases
ProteinModelPortal	A2ST90.
ModBase	Search...
Protein-protein interaction databases
STRING	A2ST90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4794418.
GenomeReviews	Gene locus Mlab_1380 in contig CP000559_GR.
KEGG	mla:Mlab_1380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	A2ST90.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MLAB410358:MLAB_1380-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METLZ

Accession

Primary (citable) accession number: A2ST90

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 6, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program