A2ST64 (A2ST64_METLZ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Mlab_1353

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. RuleBase RU004252 HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 Nucleotidyltransferase EMBL ABN07520.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	35 – 42	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	37	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A2ST64 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 67EFF8ECBAEF0FA6	FASTA	251	27,284
	10 20 30 40 50 60 MTEVTCECIG CEIVKTKDLI RQTVWEANAR GVVIGISGGI DSAVACSLCC KALSPERVLG 70 80 90 100 110 120 VNMPVSSNNP QDHLDAEELC RGLGVELITV PLEDVRAAFL AAPHITDTPV LRGNIAARLR 130 140 150 160 170 180 MTTLYNITAA RKYLVCGTSN KTEYMIGYST KWGDSAADIQ PLLHLWKKDV YLLAKELGVP 190 200 210 220 230 240 ESIIRKAPSA GFWEGQSDEG ELGMSYADLD AALIELEKNG GVPNNPNEEK ALGFIQRSRH 250 KRAPAANRLF T

References

[1]

"Complete genome sequence of Methanocorpusculum labreanum type strain Z." Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:197-203(2009) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000559 Genomic DNA. Translation: ABN07520.1.
RefSeq	YP_001030787.1. NC_008942.1.
3D structure databases
ProteinModelPortal	A2ST64.
ModBase	Search...
Protein-protein interaction databases
STRING	A2ST64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4795258.
GenomeReviews	Gene locus Mlab_1353 in contig CP000559_GR.
KEGG	mla:Mlab_1353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	YDISARD.
PhylomeDB	A2ST64.
ProtClustDB	CLSK949856.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A2ST64_METLZ

Accession

Primary (citable) accession number: A2ST64

Entry history

Integrated into UniProtKB/TrEMBL:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)