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Protein

Enolase

Gene

eno

Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144SubstrateUniRule annotation1
Binding sitei153SubstrateUniRule annotation1
Active sitei194Proton donorUniRule annotation1
Metal bindingi230MagnesiumUniRule annotation1
Metal bindingi273MagnesiumUniRule annotation1
Binding sitei273SubstrateUniRule annotation1
Metal bindingi301MagnesiumUniRule annotation1
Binding sitei301SubstrateUniRule annotation1
Active sitei326Proton acceptorUniRule annotation1
Binding sitei326Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei377SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:Mlab_1238
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
Proteomesi
  • UP000000365 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000192191 – 399EnolaseAdd BLAST399

Interactioni

Protein-protein interaction databases

STRINGi410358.Mlab_1238.

Structurei

3D structure databases

ProteinModelPortaliA2SSV1.
SMRiA2SSV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 356Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169. Archaea.
COG0148. LUCA.
HOGENOMiHOG000072173.
KOiK01689.
OMAiYVEDPFH.
OrthoDBiPOG093Z03D9.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A2SSV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEISDILLR TILDSRGNPT VEAEISTISG GFGRACAPSG ASTGIYEAKV
60 70 80 90 100
RPCDEAVADA YINLIPKLIE LDSADQRGFD DTLHEVDGTS DLSGIGANIA
110 120 130 140 150
VALSLANAKA AASTLNMELY QYLGGAFISQ TPLPLGNVIG GGAHAVDATD
160 170 180 190 200
IQEFLIVPTG ASTAAEAVFT NALVHKRIKE ILIARGKGCG KGDEGGWAPH
210 220 230 240 250
IADLEAFEIV NEATTKVFDE TGIEVRMGLD VAASEMWDAA SGRYVYKNAK
260 270 280 290 300
RTTEEQIAYI ADLTEKYNLI YVEDPIQEED FEGFARITEE VSGRDTLICG
310 320 330 340 350
DDLFVTNASR LEEGIRNDAC NCVLIKPNQI GTLTDTFETI SLAHDYGYET
360 370 380 390
VMSHRSGETT DNTIAHLATA FGCCFMKSGV VGGERIAKLN ELIRIEEHF
Length:399
Mass (Da):42,847
Last modified:March 6, 2007 - v1
Checksum:iBD3205014E76ECB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA. Translation: ABN07407.1.
RefSeqiWP_011833610.1. NC_008942.1.

Genome annotation databases

EnsemblBacteriaiABN07407; ABN07407; Mlab_1238.
GeneIDi4795183.
KEGGimla:Mlab_1238.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_METLZ
AccessioniPrimary (citable) accession number: A2SSV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: June 7, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families