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A2SRG2 (SYE_METLZ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:Mlab_0747
OrganismMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]
Taxonomic identifier410358 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000331008

Regions

Motif108 – 11811"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
A2SRG2 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: E0D76327B9491F70

FASTA56062,583
        10         20         30         40         50         60 
MADADIRNDI YVLALENAVK HKAVPRAGAI LGSVMGAHPE LRSKAKEINA LIPEILEEVA 

        70         80         90        100        110        120 
ALSIEEREEK LMSLNPGAIE KMHEKKERSH ELPDLPNAES GVVMRFAPNP SGPLHLGHAR 

       130        140        150        160        170        180 
ASVLNDYYVK KYGGKFYYRV EDTDPKRVDP SAYEMVSEDL AWLGIGITDV VYQSDRFAIY 

       190        200        210        220        230        240 
YEYARKLLEL GGAYMCTCDN NEFREKKLKK IACPCREISV EENLKRFDDM LAGKYAEGEI 

       250        260        270        280        290        300 
TLRVKTDIAH PDPAVRDFAA MRVLTSTPHP RKPAIFVYPL MNFSVAVDDH LLGMTHVIRG 

       310        320        330        340        350        360 
KDHIANTRRQ EYIYNYFGWK MPYFYHYGRM SIAGLELSTS GMRKGINEGL YTGWDDIHLG 

       370        380        390        400        410        420 
TLRALARRGI QPEAVRAATI DIGMGDTDIS FSWENLFAQN KAVIDAGADR YFFVPDAVEV 

       430        440        450        460        470        480 
EIAGAPKTEA HAPVYPNQPE RGERVLPFTG RVLLPKSEME KGGMLRLKDL FNINITGPNS 

       490        500        510        520        530        540 
AEYAGDSLAE ARSQKAAIVQ WLPTETAAPC SLLTPEGIQE GFSEPAVLGY LGRIVQFERV 

       550        560 
GFSKIDAVND GKVIAYFTHR 

« Hide

References

[1]"Complete genome sequence of Methanocorpusculum labreanum type strain Z."
Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.
Stand. Genomic Sci. 1:197-203(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000559 Genomic DNA. Translation: ABN06918.1.
RefSeqYP_001030185.1. NC_008942.1.

3D structure databases

ProteinModelPortalA2SRG2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING410358.Mlab_0747.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN06918; ABN06918; Mlab_0747.
GeneID4795118.
KEGGmla:Mlab_0747.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAMRFAPNP.
ProtClustDBPRK04156.

Enzyme and pathway databases

BioCycMLAB410358:GH7C-768-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_METLZ
AccessionPrimary (citable) accession number: A2SRG2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 6, 2007
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries