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A2SQM6 (TFE_METLZ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor E

Short name=TFE
Alternative name(s):
TFIIE subunit alpha homolog
Transcription initiation factor TFIIE
Gene names
Name:tfe
Ordered Locus Names:Mlab_0458
OrganismMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]
Taxonomic identifier410358 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes By similarity. HAMAP-Rule MF_01909

Subunit structure

Monomer By similarity. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes By similarity. HAMAP-Rule MF_01909

Domain

The winged helix domain is involved in binding to DNA in the preinitiation complex By similarity. HAMAP-Rule MF_01909

Sequence similarities

Belongs to the TFE family.

Contains 1 HTH TFE/IIEalpha-type domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211Transcription factor E HAMAP-Rule MF_01909
PRO_0000326603

Regions

Domain10 – 130121HTH TFE/IIEalpha-type

Sequences

Sequence LengthMass (Da)Tools
A2SQM6 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 52812E41EF45E768

FASTA21124,545
        10         20         30         40         50         60 
MTVITEEQLG NPAIYQYLLK LVGEEGLELL RRWSDVSYAR RWVEEKLSSE DLKAADKARF 

        70         80         90        100        110        120 
LAESRRSDED LVEAERSDEE IAELTGINLN SVRHTLYNLY EHRLAEYRRI KNNETGWLTY 

       130        140        150        160        170        180 
LWLMRMDHMN MVLRNEMEVA AGKLAGRLRY DEANDFYQCK NCGITTTFNN AMTTNFSCPQ 

       190        200        210 
CGTMLVHFDD ELIITALKKR LAKMQTALDN A 

« Hide

References

[1]"Complete genome sequence of Methanocorpusculum labreanum type strain Z."
Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.
Stand. Genomic Sci. 1:197-203(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000559 Genomic DNA. Translation: ABN06632.1.
RefSeqYP_001029899.1. NC_008942.1.

3D structure databases

ProteinModelPortalA2SQM6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING410358.Mlab_0458.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN06632; ABN06632; Mlab_0458.
GeneID4794695.
KEGGmla:Mlab_0458.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1675.
HOGENOMHOG000015477.
KOK03136.
OMAFENDFLC.
ProtClustDBCLSK949888.

Enzyme and pathway databases

BioCycMLAB410358:GH7C-474-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 2 hits.
HAMAPMF_01909. TFE_arch.
InterProIPR016481. TF_E_archaea.
IPR017919. TFIIE/TFIIEa_HTH.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamPF08271. TF_Zn_Ribbon. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
PROSITEPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTFE_METLZ
AccessionPrimary (citable) accession number: A2SQM6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 6, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families