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Protein

Transcription factor E

Gene

tfe

Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.UniRule annotation

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. DNA-templated transcription, initiation Source: UniProtKB-HAMAP
  2. regulation of transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMLAB410358:GH7C-474-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor EUniRule annotation
Short name:
TFEUniRule annotation
Alternative name(s):
TFIIE subunit alpha homologUniRule annotation
Transcription initiation factor TFIIEUniRule annotation
Gene namesi
Name:tfeUniRule annotation
Ordered Locus Names:Mlab_0458
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
ProteomesiUP000000365: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Transcription factor EPRO_0000326603Add
BLAST

Interactioni

Subunit structurei

Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.UniRule annotation

Protein-protein interaction databases

STRINGi410358.Mlab_0458.

Structurei

3D structure databases

ProteinModelPortaliA2SQM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 130121HTH TFE/IIEalpha-typeUniRule annotationAdd
BLAST

Domaini

The winged helix domain is involved in binding to DNA in the preinitiation complex.UniRule annotation

Sequence similaritiesi

Belongs to the TFE family.UniRule annotation
Contains 1 HTH TFE/IIEalpha-type domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1675.
HOGENOMiHOG000015477.
KOiK03136.
OMAiFENDFLC.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_01909. TFE_arch.
InterProiIPR016481. TF_E_archaea.
IPR017919. TFIIE/TFIIEa_HTH.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2SQM6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVITEEQLG NPAIYQYLLK LVGEEGLELL RRWSDVSYAR RWVEEKLSSE
60 70 80 90 100
DLKAADKARF LAESRRSDED LVEAERSDEE IAELTGINLN SVRHTLYNLY
110 120 130 140 150
EHRLAEYRRI KNNETGWLTY LWLMRMDHMN MVLRNEMEVA AGKLAGRLRY
160 170 180 190 200
DEANDFYQCK NCGITTTFNN AMTTNFSCPQ CGTMLVHFDD ELIITALKKR
210
LAKMQTALDN A
Length:211
Mass (Da):24,545
Last modified:March 6, 2007 - v1
Checksum:i52812E41EF45E768
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA. Translation: ABN06632.1.
RefSeqiYP_001029899.1. NC_008942.1.

Genome annotation databases

EnsemblBacteriaiABN06632; ABN06632; Mlab_0458.
GeneIDi4794695.
KEGGimla:Mlab_0458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA. Translation: ABN06632.1.
RefSeqiYP_001029899.1. NC_008942.1.

3D structure databases

ProteinModelPortaliA2SQM6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi410358.Mlab_0458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN06632; ABN06632; Mlab_0458.
GeneIDi4794695.
KEGGimla:Mlab_0458.

Phylogenomic databases

eggNOGiCOG1675.
HOGENOMiHOG000015477.
KOiK03136.
OMAiFENDFLC.

Enzyme and pathway databases

BioCyciMLAB410358:GH7C-474-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_01909. TFE_arch.
InterProiIPR016481. TF_E_archaea.
IPR017919. TFIIE/TFIIEa_HTH.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43576 / DSM 4855 / Z.

Entry informationi

Entry nameiTFE_METLZ
AccessioniPrimary (citable) accession number: A2SQM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 6, 2007
Last modified: February 4, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.