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Protein

Transcription factor E

Gene

tfe

Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciMLAB410358:G1G83-485-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor EUniRule annotation
Short name:
TFEUniRule annotation
Alternative name(s):
TFIIE subunit alpha homologUniRule annotation
Transcription initiation factor TFIIEUniRule annotation
Gene namesi
Name:tfeUniRule annotation
Ordered Locus Names:Mlab_0458
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
Proteomesi
  • UP000000365 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003266031 – 211Transcription factor EAdd BLAST211

Interactioni

Subunit structurei

Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.UniRule annotation

Protein-protein interaction databases

STRINGi410358.Mlab_0458

Structurei

3D structure databases

ProteinModelPortaliA2SQM6
SMRiA2SQM6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 130HTH TFE/IIEalpha-typeUniRule annotationAdd BLAST121

Domaini

The winged helix domain is involved in binding to DNA in the preinitiation complex.UniRule annotation

Sequence similaritiesi

Belongs to the TFE family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04270 Archaea
COG1675 LUCA
HOGENOMiHOG000015477
KOiK03136
OrthoDBiPOG093Z0C2C

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_01909 TFE_arch, 1 hit
InterProiView protein in InterPro
IPR016481 TF_E_archaea
IPR017919 TFIIE/TFIIEa_HTH
IPR002853 TFIIE_asu
IPR024550 TFIIEa/SarR/Rpc3_HTH_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
IPR013137 Znf_TFIIB
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
PF02002 TFIIE_alpha, 1 hit
PIRSFiPIRSF006373 TF_E_archaea, 1 hit
SMARTiView protein in SMART
SM00531 TFIIE, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS51344 HTH_TFE_IIE, 1 hit

Sequencei

Sequence statusi: Complete.

A2SQM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVITEEQLG NPAIYQYLLK LVGEEGLELL RRWSDVSYAR RWVEEKLSSE
60 70 80 90 100
DLKAADKARF LAESRRSDED LVEAERSDEE IAELTGINLN SVRHTLYNLY
110 120 130 140 150
EHRLAEYRRI KNNETGWLTY LWLMRMDHMN MVLRNEMEVA AGKLAGRLRY
160 170 180 190 200
DEANDFYQCK NCGITTTFNN AMTTNFSCPQ CGTMLVHFDD ELIITALKKR
210
LAKMQTALDN A
Length:211
Mass (Da):24,545
Last modified:March 6, 2007 - v1
Checksum:i52812E41EF45E768
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA Translation: ABN06632.1
RefSeqiWP_011832833.1, NC_008942.1

Genome annotation databases

EnsemblBacteriaiABN06632; ABN06632; Mlab_0458
GeneIDi4794695
KEGGimla:Mlab_0458

Similar proteinsi

Entry informationi

Entry nameiTFE_METLZ
AccessioniPrimary (citable) accession number: A2SQM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 6, 2007
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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