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Protein

NAD kinase

Gene

nadK

Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Proton acceptorUniRule annotation
Binding sitei69 – 691NADUniRule annotation
Binding sitei143 – 1431NADUniRule annotation
Binding sitei160 – 1601NADUniRule annotation
Binding sitei162 – 1621NADUniRule annotation
Binding sitei197 – 1971NAD; via carbonyl oxygenUniRule annotation
Binding sitei231 – 2311NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 652NADUniRule annotation
Nucleotide bindingi132 – 1332NADUniRule annotation
Nucleotide bindingi173 – 1786NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLAB410358:GH7C-443-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Mlab_0428
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
ProteomesiUP000000365 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271NAD kinasePRO_1000059879Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi410358.Mlab_0428.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiHGPRRGH.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

A2SQJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKICIVSRID LKEPVELAQS LGWMLRDQGH DVVYEQSVAA ELGYAPVSLS
60 70 80 90 100
KDFSADLIVV LGGDGSVLRT IRMLDHQVPV VGINQGQVGF LTDIERDKAE
110 120 130 140 150
EILTSLSLPL PLDPRMRISI EFNGRSVGSA LNEAVIVTSR PAKILKFAVF
160 170 180 190 200
VNGRQIDEFR ADGLIIGTPT GSTAYAMSAG GPIVDSTIEA MLLVPLAPYM
210 220 230 240 250
LSSRPYLINS NSEVEIRLVS VKPALLVIDG QDQYEIGENA TLLIRKSPDP
260 270
ALFVDVGRGF FDKVEQKLRL L
Length:271
Mass (Da):29,533
Last modified:March 6, 2007 - v1
Checksum:i9FFA2446BF73A4D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA. Translation: ABN06604.1.
RefSeqiYP_001029871.1. NC_008942.1.

Genome annotation databases

EnsemblBacteriaiABN06604; ABN06604; Mlab_0428.
GeneIDi4794383.
KEGGimla:Mlab_0428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA. Translation: ABN06604.1.
RefSeqiYP_001029871.1. NC_008942.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi410358.Mlab_0428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN06604; ABN06604; Mlab_0428.
GeneIDi4794383.
KEGGimla:Mlab_0428.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiHGPRRGH.

Enzyme and pathway databases

BioCyciMLAB410358:GH7C-443-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43576 / DSM 4855 / Z.

Entry informationi

Entry nameiNADK_METLZ
AccessioniPrimary (citable) accession number: A2SQJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 6, 2007
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.