A2SQG6 (RIBB_METLZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase
Short name=DHBP synthase
EC=4.1.99.12

Gene names

Name:	ribB
Ordered Locus Names:	Mlab_0396

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_00180
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180
Subunit structure	Homodimer By similarity. HAMAP MF_00180
Sequence similarities	Belongs to the DHBP synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180		PRO_1000040616
Regions
	Region	28 – 29	2	Substrate binding By similarity
	Region	140 – 144	5	Substrate binding By similarity
Sites
	Metal binding	29	1	Magnesium or manganese 1 By similarity
	Metal binding	29	1	Magnesium or manganese 2 By similarity
	Metal binding	143	1	Magnesium or manganese 2 By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Site	126	1	Essential for catalytic activity By similarity
	Site	164	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SQG6 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: B0B73ECB697BAACC	FASTA	219	24,522
	10 20 30 40 50 60 MFTFNTIEEA LKSLQNGEMI IVTDDENREN EGDLICAAEY ATTENVNFMA KYGRGLICMP 70 80 90 100 110 120 MGRSLVEKLC LPPMVLKNTD NHETAFTVSI DHVDTTTGIS AVERGITARK CIDPNARPED 130 140 150 160 170 180 FRRPGHMFPL QAKDNGVFER EGHTEATVDL MKLAGLREAG LCCEIMADNG EMMRTPELIS 190 200 210 MAKQYNLTFV TIKDLQAYRR KQEASALAVE QKNMSPMDC

References

[1]

"Complete genome sequence of Methanocorpusculum labreanum type strain Z." Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:197-203(2009) [PubMed: 21304657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000559 Genomic DNA. Translation: ABN06572.1.
RefSeq	YP_001029839.1. NC_008942.1.
3D structure databases
ProteinModelPortal	A2SQG6.
ModBase	Search...
Protein-protein interaction databases
STRING	A2SQG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4794681.
GenomeReviews	Gene locus Mlab_0396 in contig CP000559_GR.
KEGG	mla:Mlab_0396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05755.
HOGENOM	HBG735778.
OMA	GDMIFAA.
ProtClustDB	CLSK964676.
Enzyme and pathway databases
BioCyc	MLAB410358:MLAB_0396-MONOMER.
Family and domain databases
HAMAP	MF_00180. RibB. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KO	K03738.
Pfam	PF00926. DHBP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMs	TIGR00506. RibB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_METLZ

Accession

Primary (citable) accession number: A2SQG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	November 16, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program