A2SQG5 (RISB_METLZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	Mlab_0395

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000040447

Sequences

	Sequence		Length	Mass (Da)
	A2SQG5 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: BC168C0FF0AE166B	FASTA	155	16,584
	10 20 30 40 50 60 MKVYEGNLVS KNIKIGIVAA RFNEFIVSKL LGGALDALKR HDVADDDVEI AWVPGAFEIP 70 80 90 100 110 120 LIAEKMAESK KYDAVICLGA VIRGSTSHYD YVCNEVTKGI ATVSLKSGIP VMFGVLTTDN 130 140 150 LEQAIERAGS KVGNKGFDAA VGAIEMVNVI RQLGN

References

[1]

"Complete genome sequence of Methanocorpusculum labreanum type strain Z." Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:197-203(2009) [PubMed: 21304657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000559 Genomic DNA. Translation: ABN06571.1.
RefSeq	YP_001029838.1. NC_008942.1.
3D structure databases
ProteinModelPortal	A2SQG5.
SMR	A2SQG5. Positions 1-153.
ModBase	Search...
Protein-protein interaction databases
STRING	A2SQG5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4794685.
GenomeReviews	Gene locus Mlab_0395 in contig CP000559_GR.
KEGG	mla:Mlab_0395.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG09975.
HOGENOM	HBG311126.
OMA	AGECSSG.
PhylomeDB	A2SQG5.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	MLAB410358:MLAB_0395-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_METLZ

Accession

Primary (citable) accession number: A2SQG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	November 16, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program