A2SQ86 (PUR2_METLZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Mlab_0316

Organism

Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]

Taxonomic identifier

410358 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_1000018823
Regions
	Domain	109 – 314	206	ATP-grasp
	Nucleotide binding	136 – 192	57	ATP By similarity
Sites
	Metal binding	272	1	Magnesium or manganese 1 By similarity
	Metal binding	284	1	Magnesium or manganese 1 By similarity
	Metal binding	284	1	Magnesium or manganese 2 By similarity
	Metal binding	286	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SQ86 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 0191C23BB1CABECF	FASTA	430	46,326
	10 20 30 40 50 60 MKILVAGGGG REHAICLALT KNANVELYSV MGKKNPGIAK IARESFIHAE TDVPAVLAFA 70 80 90 100 110 120 KEHNIQYAVV GPEAPLEAGL VDALTKAGIG CVGPVKAAAR IETDKGFCRG LMNKYGIDGC 130 140 150 160 170 180 PAYKLCNTPK EAAEFIREYP GDLAVKPTGL TGGKGVKVMG EQVDREGAVE YAMTLKDQVI 190 200 210 220 230 240 ILEERLLGEE FTLMAFVDGK HLVPMPLVQD HKRAFEGDVG PNTGGMGSYS LENHKFPFVT 250 260 270 280 290 300 EDDYAKAISI MQATIDALAQ EGSPYKGILY GQFMNTKTGP KVIEFNARFG DPEAMNVLSI 310 320 330 340 350 360 LTSDFLTIAE HIINGTLSAN DVSFEEKATV CKYIVPMDYP DKPHAGDVIT VGPAENTILY 370 380 390 400 410 420 YANIALENGV LMTLTSRTMA YVGIGASLAE AEKYAEAACR NVSGNVRYRS DIGTEALFAK 430 RIAHMKELRS

References

[1]

"Complete genome sequence of Methanocorpusculum labreanum type strain Z." Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:197-203(2009) [PubMed: 21304657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000559 Genomic DNA. Translation: ABN06492.1.
RefSeq	YP_001029759.1. NC_008942.1.
3D structure databases
ProteinModelPortal	A2SQ86.
ModBase	Search...
Protein-protein interaction databases
STRING	A2SQ86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4795228.
GenomeReviews	Gene locus Mlab_0316 in contig CP000559_GR.
KEGG	mla:Mlab_0316.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04186.
HOGENOM	HBG404060.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	MLAB410358:MLAB_0316-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01945.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METLZ

Accession

Primary (citable) accession number: A2SQ86

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	November 16, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program