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A2SPV6

- SYI_METLZ

UniProt

A2SPV6 - SYI_METLZ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMLAB410358:GH7C-189-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mlab_0186
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
ProteomesiUP000000365: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Isoleucine--tRNA ligasePRO_1000022155Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi410358.Mlab_0186.

Structurei

3D structure databases

ProteinModelPortaliA2SPV6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2SPV6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEISESYVP AVVENEVREY WKANNTYRET RKLHESGKPW LFVDGPPYTT
60 70 80 90 100
GYIHLGTAWN KILKDAILRY HSMTGQHIIE RAGYDMHGLP IEVKVEEKLG
110 120 130 140 150
FKNKADIEKY GVAKFIEECR EFALTHKDLM SEQFKDLGTW MDFDDPYQTV
160 170 180 190 200
DKGYIEAAWY TLKRCEEEKM LERGSRVVNW CPRCGTAIAD AEVEYWDETD
210 220 230 240 250
PSIFVKFPIQ GTENEYLVIW TTTPWTLPAN VAVAVGEEFV YAKCRAVKDG
260 270 280 290 300
KSEDLWIAKE LAEQILKYGK YQDYSIIETK TGAELAGTKY ISPLASAVPM
310 320 330 340 350
QAQIEHRVVI ADYVAMENTG MVHIAPGHGW DDYLVGLKEN LPAVCPVDGN
360 370 380 390 400
GNFTDEAGIF AGKYVKAPET NQEVIDVLGD AMLAVRKITH RYGHCWRCKT
410 420 430 440 450
PIIYRATSQW FLKVKDIREK MLEEIADEVT WYPEWAGSAR FHDWVEEARD
460 470 480 490 500
WCISRQRYWG IPIPVWVCPV CNKYHVVGRY EELEQLSGQK MTDPHRPYVD
510 520 530 540 550
DITIPCECGG TMKRIPDIFD VWYDSGIASW ATLRFPEKPE DFGKYWPADF
560 570 580 590 600
ILEGHDQTRG WFYSQLALST IAFGKAPYKS VLMHGFALDA EGKKMSKSLG
610 620 630 640 650
NVIAPEDVAK QFGVDVMRQY ILSANAPWDD MRFSLEGVKT NHRMFNVLWN
660 670 680 690 700
VYKFPLPYMA LDGYKPAAKD GVWDPSAVED HISEFCREDR WLISRVNSLA
710 720 730 740 750
EQVTKEMEVC NLHRATRPIS TFILDELSRW YVQLVRPRMW LEEESVSKMQ
760 770 780 790 800
AYDTMYYVMR RLVTIFAPFA PHITECMYQN LRCEGDLPSV HMVDWFSGND
810 820 830 840 850
ALRDPVLEEE MEIVQEFDEA VANARQNGKR KGRWPVGTVV VATDSEKVAG
860 870 880 890 900
AVSAMNDMCC DRANARTVTV VKGVWDKLDW TAVPVMKVIG KQFGRDGPKV
910 920 930 940 950
KAFIEEANGT KLKALLTADG KVSMEKDGFT AELTEEHMTF EEKMPENIFS
960 970 980 990 1000
SPMENGTIYV DVTLTPELEA EGYSREVIRR IQEMRKQAGL AVDAKIKAEV
1010 1020 1030 1040 1050
VIDDARVMPL VDSKHDVIET EVRANCLKIR VPDGETCSCR VADEAILAMD
1060
WEIDDLKVRI SISKAE
Length:1,066
Mass (Da):121,910
Last modified:March 6, 2007 - v1
Checksum:i7362A12BEBD8228A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA. Translation: ABN06362.1.
RefSeqiYP_001029629.1. NC_008942.1.

Genome annotation databases

EnsemblBacteriaiABN06362; ABN06362; Mlab_0186.
GeneIDi4795794.
KEGGimla:Mlab_0186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA. Translation: ABN06362.1 .
RefSeqi YP_001029629.1. NC_008942.1.

3D structure databases

ProteinModelPortali A2SPV6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 410358.Mlab_0186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN06362 ; ABN06362 ; Mlab_0186 .
GeneIDi 4795794.
KEGGi mla:Mlab_0186.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.

Enzyme and pathway databases

BioCyci MLAB410358:GH7C-189-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43576 / DSM 4855 / Z.

Entry informationi

Entry nameiSYI_METLZ
AccessioniPrimary (citable) accession number: A2SPV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3