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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei597ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMLAB410358:G1G83-193-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mlab_0186
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
Proteomesi
  • UP000000365 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221551 – 1066Isoleucine--tRNA ligaseAdd BLAST1066

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi410358.Mlab_0186

Structurei

3D structure databases

ProteinModelPortaliA2SPV6
SMRiA2SPV6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A2SPV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEISESYVP AVVENEVREY WKANNTYRET RKLHESGKPW LFVDGPPYTT
60 70 80 90 100
GYIHLGTAWN KILKDAILRY HSMTGQHIIE RAGYDMHGLP IEVKVEEKLG
110 120 130 140 150
FKNKADIEKY GVAKFIEECR EFALTHKDLM SEQFKDLGTW MDFDDPYQTV
160 170 180 190 200
DKGYIEAAWY TLKRCEEEKM LERGSRVVNW CPRCGTAIAD AEVEYWDETD
210 220 230 240 250
PSIFVKFPIQ GTENEYLVIW TTTPWTLPAN VAVAVGEEFV YAKCRAVKDG
260 270 280 290 300
KSEDLWIAKE LAEQILKYGK YQDYSIIETK TGAELAGTKY ISPLASAVPM
310 320 330 340 350
QAQIEHRVVI ADYVAMENTG MVHIAPGHGW DDYLVGLKEN LPAVCPVDGN
360 370 380 390 400
GNFTDEAGIF AGKYVKAPET NQEVIDVLGD AMLAVRKITH RYGHCWRCKT
410 420 430 440 450
PIIYRATSQW FLKVKDIREK MLEEIADEVT WYPEWAGSAR FHDWVEEARD
460 470 480 490 500
WCISRQRYWG IPIPVWVCPV CNKYHVVGRY EELEQLSGQK MTDPHRPYVD
510 520 530 540 550
DITIPCECGG TMKRIPDIFD VWYDSGIASW ATLRFPEKPE DFGKYWPADF
560 570 580 590 600
ILEGHDQTRG WFYSQLALST IAFGKAPYKS VLMHGFALDA EGKKMSKSLG
610 620 630 640 650
NVIAPEDVAK QFGVDVMRQY ILSANAPWDD MRFSLEGVKT NHRMFNVLWN
660 670 680 690 700
VYKFPLPYMA LDGYKPAAKD GVWDPSAVED HISEFCREDR WLISRVNSLA
710 720 730 740 750
EQVTKEMEVC NLHRATRPIS TFILDELSRW YVQLVRPRMW LEEESVSKMQ
760 770 780 790 800
AYDTMYYVMR RLVTIFAPFA PHITECMYQN LRCEGDLPSV HMVDWFSGND
810 820 830 840 850
ALRDPVLEEE MEIVQEFDEA VANARQNGKR KGRWPVGTVV VATDSEKVAG
860 870 880 890 900
AVSAMNDMCC DRANARTVTV VKGVWDKLDW TAVPVMKVIG KQFGRDGPKV
910 920 930 940 950
KAFIEEANGT KLKALLTADG KVSMEKDGFT AELTEEHMTF EEKMPENIFS
960 970 980 990 1000
SPMENGTIYV DVTLTPELEA EGYSREVIRR IQEMRKQAGL AVDAKIKAEV
1010 1020 1030 1040 1050
VIDDARVMPL VDSKHDVIET EVRANCLKIR VPDGETCSCR VADEAILAMD
1060
WEIDDLKVRI SISKAE
Length:1,066
Mass (Da):121,910
Last modified:March 6, 2007 - v1
Checksum:i7362A12BEBD8228A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA Translation: ABN06362.1
RefSeqiWP_011832563.1, NC_008942.1

Genome annotation databases

EnsemblBacteriaiABN06362; ABN06362; Mlab_0186
GeneIDi4795794
KEGGimla:Mlab_0186

Similar proteinsi

Entry informationi

Entry nameiSYI_METLZ
AccessioniPrimary (citable) accession number: A2SPV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health