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A2SPV6

- SYI_METLZ

UniProt

A2SPV6 - SYI_METLZ

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Mlab_0186
Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMLAB410358:GH7C-189-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Mlab_0186
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
ProteomesiUP000000365: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Isoleucine--tRNA ligaseUniRule annotationPRO_1000022155Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi410358.Mlab_0186.

Structurei

3D structure databases

ProteinModelPortaliA2SPV6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotationAdd
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2SPV6-1 [UniParc]FASTAAdd to Basket

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MKEISESYVP AVVENEVREY WKANNTYRET RKLHESGKPW LFVDGPPYTT     50
GYIHLGTAWN KILKDAILRY HSMTGQHIIE RAGYDMHGLP IEVKVEEKLG 100
FKNKADIEKY GVAKFIEECR EFALTHKDLM SEQFKDLGTW MDFDDPYQTV 150
DKGYIEAAWY TLKRCEEEKM LERGSRVVNW CPRCGTAIAD AEVEYWDETD 200
PSIFVKFPIQ GTENEYLVIW TTTPWTLPAN VAVAVGEEFV YAKCRAVKDG 250
KSEDLWIAKE LAEQILKYGK YQDYSIIETK TGAELAGTKY ISPLASAVPM 300
QAQIEHRVVI ADYVAMENTG MVHIAPGHGW DDYLVGLKEN LPAVCPVDGN 350
GNFTDEAGIF AGKYVKAPET NQEVIDVLGD AMLAVRKITH RYGHCWRCKT 400
PIIYRATSQW FLKVKDIREK MLEEIADEVT WYPEWAGSAR FHDWVEEARD 450
WCISRQRYWG IPIPVWVCPV CNKYHVVGRY EELEQLSGQK MTDPHRPYVD 500
DITIPCECGG TMKRIPDIFD VWYDSGIASW ATLRFPEKPE DFGKYWPADF 550
ILEGHDQTRG WFYSQLALST IAFGKAPYKS VLMHGFALDA EGKKMSKSLG 600
NVIAPEDVAK QFGVDVMRQY ILSANAPWDD MRFSLEGVKT NHRMFNVLWN 650
VYKFPLPYMA LDGYKPAAKD GVWDPSAVED HISEFCREDR WLISRVNSLA 700
EQVTKEMEVC NLHRATRPIS TFILDELSRW YVQLVRPRMW LEEESVSKMQ 750
AYDTMYYVMR RLVTIFAPFA PHITECMYQN LRCEGDLPSV HMVDWFSGND 800
ALRDPVLEEE MEIVQEFDEA VANARQNGKR KGRWPVGTVV VATDSEKVAG 850
AVSAMNDMCC DRANARTVTV VKGVWDKLDW TAVPVMKVIG KQFGRDGPKV 900
KAFIEEANGT KLKALLTADG KVSMEKDGFT AELTEEHMTF EEKMPENIFS 950
SPMENGTIYV DVTLTPELEA EGYSREVIRR IQEMRKQAGL AVDAKIKAEV 1000
VIDDARVMPL VDSKHDVIET EVRANCLKIR VPDGETCSCR VADEAILAMD 1050
WEIDDLKVRI SISKAE 1066
Length:1,066
Mass (Da):121,910
Last modified:March 6, 2007 - v1
Checksum:i7362A12BEBD8228A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000559 Genomic DNA. Translation: ABN06362.1.
RefSeqiWP_011832563.1. NC_008942.1.
YP_001029629.1. NC_008942.1.

Genome annotation databases

EnsemblBacteriaiABN06362; ABN06362; Mlab_0186.
GeneIDi4795794.
KEGGimla:Mlab_0186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000559 Genomic DNA. Translation: ABN06362.1 .
RefSeqi WP_011832563.1. NC_008942.1.
YP_001029629.1. NC_008942.1.

3D structure databases

ProteinModelPortali A2SPV6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 410358.Mlab_0186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN06362 ; ABN06362 ; Mlab_0186 .
GeneIDi 4795794.
KEGGi mla:Mlab_0186.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.

Enzyme and pathway databases

BioCyci MLAB410358:GH7C-189-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43576 / DSM 4855 / Z.

Entry informationi

Entry nameiSYI_METLZ
AccessioniPrimary (citable) accession number: A2SPV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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