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A2SPI6 (A2SPI6_METLZ) Unreviewed, UniProtKB/TrEMBL

Last modified November 16, 2011. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase HAMAP MF_00011
Short name=AMPSase HAMAP MF_00011
Short name=AdSS HAMAP MF_00011
EC=6.3.4.4 HAMAP MF_00011
Alternative name(s):
IMP--aspartate ligase HAMAP MF_00011
Gene names
Name:purA HAMAP MF_00011
Ordered Locus Names:Mlab_0064
OrganismMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) [Complete proteome] [HAMAP]
Taxonomic identifier410358 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanocorpusculaceae › Methanocorpusculum

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity. RuleBase RU000520

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011 RuleBase RU000520

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011 RuleBase RU000520

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family. RuleBase RU004163 HAMAP MF_00011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding12 – 187GTP By similarity HAMAP MF_00011
Nucleotide binding42 – 443GTP By similarity HAMAP MF_00011
Nucleotide binding281 – 2833GTP By similarity HAMAP MF_00011
Nucleotide binding321 – 3233GTP By similarity HAMAP MF_00011
Region13 – 164IMP binding By similarity HAMAP MF_00011
Region40 – 434IMP binding By similarity HAMAP MF_00011
Region249 – 2557Substrate binding By similarity HAMAP MF_00011

Sites

Active site131Proton acceptor By similarity HAMAP MF_00011
Active site431Proton donor By similarity HAMAP MF_00011
Metal binding131Magnesium By similarity HAMAP MF_00011
Metal binding421Magnesium; via carbonyl oxygen By similarity HAMAP MF_00011
Binding site1241IMP By similarity HAMAP MF_00011
Binding site1381IMP; shared with dimeric partner By similarity HAMAP MF_00011
Binding site1761IMP By similarity HAMAP MF_00011
Binding site1911IMP By similarity HAMAP MF_00011
Binding site2531IMP By similarity HAMAP MF_00011
Binding site2551GTP By similarity HAMAP MF_00011

Sequences

Sequence LengthMass (Da)Tools
A2SPI6 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 3DCEEF2E95E479E6

FASTA33636,027
        10         20         30         40         50         60 
MPSTIIVGGF FGDEGKGKIV AHIAQKDQAT IIARGGVGPN AGHTINVQNE KYVVRMIPSG 

        70         80         90        100        110        120 
FLYPKAELMI GSGVLVDPRV FAHELDILKC AGRTRIDSRC GIIEEEHILL DKKDPHLSKT 

       130        140        150        160        170        180 
VGSTGSGCGP ANSDRVMRKG RLARDVPELA PYITDVAQNV NDAIEKGDSI LIEGTQGFGI 

       190        200        210        220        230        240 
SLYYGNYPFV TSKDTSASQM AADVGVGPTN IDDVVVVFKA FPTRVGEGPF PTELTHDESI 

       250        260        270        280        290        300 
ALGIQEFGAV THRERRIGMW DGKMARYSAM INGCTTIAIT GIDHIDESVY GATDYAKLTP 

       310        320        330 
KALAFIDQIE ADTGKPVGII STGPDQSHII DIRSEL

« Hide

References

[1]"Complete genome sequence of Methanocorpusculum labreanum type strain Z."
Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L., Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.
Stand. Genomic Sci. 1:197-203(2009)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43576 / DSM 4855 / Z.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000559 Genomic DNA. Translation: ABN06242.1.
RefSeqYP_001029509.1. NC_008942.1.

3D structure databases

ProteinModelPortalA2SPI6.
SMRA2SPI6. Positions 1-333.
ModBaseSearch...

Protein-protein interaction databases

STRINGA2SPI6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4795635.
GenomeReviewsGene locus Mlab_0064 in contig CP000559_GR.
KEGGmla:Mlab_0064.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04146.
HOGENOMHBG658237.
OMAYVLGIIK.
ProtClustDBPRK04293.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 2 hits.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
PROSITEPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA2SPI6_METLZ
AccessionPrimary (citable) accession number: A2SPI6
Entry history
Integrated into UniProtKB/TrEMBL: March 6, 2007
Last sequence update: March 6, 2007
Last modified: November 16, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info