A2SL82 (ACEK_METPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Mpe_A3368

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Methylibium

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288289
Regions
	Nucleotide binding	332 – 338	7	ATP By similarity
Sites
	Active site	388	1	By similarity
	Binding site	353	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SL82 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 125DFFC60B681FAD	FASTA	618	72,031
	10 20 30 40 50 60 MAGSFVTTLN DPRAFDIAQA LLDGFNRHYK LFRQTSAEAK QRFEAADWHG QQRAQRERIE 70 80 90 100 110 120 FYDLRVDEAA ERLENEFRAS SLSEETWQQV KLYYIGLLIN HHQPELAETF FNSVTTKILH 130 140 150 160 170 180 RSYFRNDFIF VRPAVSTEYI ENEEPDSLPT YRAYYPSRPG SAEGLRETLL RIVDNYQLQR 190 200 210 220 230 240 EFEDLGRDID YVLQAFRNQF GDVKLSANFQ IQVLASLFFR NKGAYIVGKV INGFRETGFA 250 260 270 280 290 300 LPVLHNSREL LTIDTALFGE DELLLLFSFA RAYFLVDMEI PSAYVQFLRS LMPRKPRWEL 310 320 330 340 350 360 YNALGLQKQG KNQFYRDFLY HLRHSSDSFR IAPGIKGMVM LVFDLPSFPF VFKVIKDFYP 370 380 390 400 410 420 PQKDTTRELI QSKYQLVKTH DRVGRMADSL EYSNVAFPRQ RFEPELIEEI RHFCPSLLEE 430 440 450 460 470 480 EGDSLVLKHL YIERRMIPLN IYLQEATPEQ MKHAVIEYGN AIKDLVAANI FPGDMLWKNF 490 500 510 520 530 540 GVTRHGKVVF YDYDEIEYLT DCNFRKVPEP RTEEEEMSGD IWYSVGPKDV FPETFGPFLL 550 560 570 580 590 600 GNPTVREVFM QHHAELLDPA FWQGRKDRIA GGYVHDVFPY DPHKRFRRPL GLGFGSLDAE 610 AVSLHPASAT TQLGHNPI

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed: 17158667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000555 Genomic DNA. Translation: ABM96321.1.
RefSeq	YP_001022556.1. NC_008825.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2SL82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4786410.
GenomeReviews	Gene locus Mpe_A3368 in contig CP000555_GR.
KEGG	mpt:Mpe_A3368.
NMPDR	fig|279263.3.peg.2954.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	A2SL82.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	MPET420662:MPE_A3368-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_METPP

Accession

Primary (citable) accession number: A2SL82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 6, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program