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A2SKX8 (FTHS_METPP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Mpe_A3264
OrganismMethylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]
Taxonomic identifier420662 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesMethylibium

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 561561Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000293046

Regions

Nucleotide binding66 – 738ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A2SKX8 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 8114C016F8B97EE1

FASTA56160,140
        10         20         30         40         50         60 
MASDIEIAQK ATLRRITQVA SDKLGIADEH LEPYGHYKAK LSLDYVDSLK DRPNGKLILV 

        70         80         90        100        110        120 
TAISPTPAGE GKTTTTVGLG DALNRIGKKT LVCLREPSLG PVFGMKGGAA GGGHAQVVPM 

       130        140        150        160        170        180 
EDINLHFTGD FNAIQLANNL LAAMIDNHIH HGNELDIDVR RITWKRVLDM NDRALRDITC 

       190        200        210        220        230        240 
SLGGPGNGYP REDGFDIVVA SEVMAIFCLA TSIQDLKERL GNIVVGYTRQ QKPVTARDLK 

       250        260        270        280        290        300 
AHGAMTVLLK DALKPNLVQT LENNPAILHG GPFANIAHGC NSVIATQTSL KLADYVVTEA 

       310        320        330        340        350        360 
GFGADLGAEK FIDIKCRKSG LRPDAVVLVA TIRALKFHGG VDVKELNTEN LDALEKGIAN 

       370        380        390        400        410        420 
IERHVANIRE HYGLPCVVSI NNFTFDTPAE LKLLQDRMAK HEVPVIVARH WAEGGKGAED 

       430        440        450        460        470        480 
VARAVVEIVE KGQSGAAGFK FVYDESLPLM DKITAIATKI YGAAKVNASA KVAGEIKKLQ 

       490        500        510        520        530        540 
DAGYGHYPVC VAKTQYSFST NPSARGAPSG HTIDIREVRL AAGAEFIVMI CGDVMTMPGL 

       550        560 
PKVPSAEKID LGDDGKVVGL F 

« Hide

References

[1]"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1."
Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R.
J. Bacteriol. 189:1931-1945(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000555 Genomic DNA. Translation: ABM96217.1.
RefSeqYP_001022452.1. NC_008825.1.

3D structure databases

ProteinModelPortalA2SKX8.
SMRA2SKX8. Positions 4-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING420662.Mpe_A3264.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM96217; ABM96217; Mpe_A3264.
GeneID4786483.
KEGGmpt:Mpe_A3264.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycMPET420662:GHBE-3302-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_METPP
AccessionPrimary (citable) accession number: A2SKX8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 6, 2007
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways