A2SKU4 (ISPE_METPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Mpe_A3230

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Methylibium

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007860
Regions
	Nucleotide binding	96 – 106	11	ATP Potential
Sites
	Active site	14	1	By similarity
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SKU4 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 56C7C1FC954D1C86	FASTA	287	30,708
	10 20 30 40 50 60 MSLQALYDVP APAKLNLFLH VTGRRDDGYH LLQSVFALID WADTLHFERR LDGRLVRHDL 70 80 90 100 110 120 AAALPEDDLC LRAARLLQRE SACPLGADIS IDKDVPWGAG LGGGSSDAAS TLLALNRLWG 130 140 150 160 170 180 LHWSRERLLA LGLQLGADVP FFVGGHNAWV EGIGERLTPI ELAPRWWAVL KPAVAVPTVA 190 200 210 220 230 240 IFGSPLLVRN TAAATISDFS ANAVEFGHND LQTPAMAYSD EVVQALALLQ SRYGASRMSG 250 260 270 280 SGSAVFAEAG TGESPTVAMM EAASLPPSWV GRMCRGLTVH PLALWAG

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed: 17158667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000555 Genomic DNA. Translation: ABM96183.1.
RefSeq	YP_001022418.1. NC_008825.1.
3D structure databases
ProteinModelPortal	A2SKU4.
ModBase	Search...
Protein-protein interaction databases
STRING	A2SKU4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4786509.
GenomeReviews	Gene locus Mpe_A3230 in contig CP000555_GR.
KEGG	mpt:Mpe_A3230.
NMPDR	fig|279263.3.peg.3851.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	LNLFLHV.
PhylomeDB	A2SKU4.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	MPET420662:MPE_A3230-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_METPP

Accession

Primary (citable) accession number: A2SKU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	November 16, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program