A2SKR5 (AROE_METPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	Mpe_A3201

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Methylibium

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000325134
Regions
	Nucleotide binding	141 – 145	5	NADP By similarity
Sites
	Active site	80	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A2SKR5 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 5006E86A5DB263F5	FASTA	294	30,206
	10 20 30 40 50 60 MKDRADAAAR DRYAVAGHPV EHSRSPFIHA RFAALTGEPI DYGRLPCPLD GFAATVSAFA 70 80 90 100 110 120 ASTGTGLGPA RGCNVTVPFK FEAVALAAQL TPRAALAQAA NTLRFDAEGW QADNTDGAGL 130 140 150 160 170 180 VRDITVHAGV ALQGATLLLV GAGGAAAGVL GPLLETGPQR LVLANRSPDR ARTLVERHAA 190 200 210 220 230 240 LAALHGVTLE AAALDDCGDG YDIVVNATAA SLAGATVPVT ARVLRPGALA LDMMYGPAAQ 250 260 270 280 290 GFLRWAAAHG AHGRDGLGML VEQAAESFWF WRGVRPPTPA VLAELRAVIE GADT

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed: 17158667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000555 Genomic DNA. Translation: ABM96154.1.
RefSeq	YP_001022389.1. NC_008825.1.
3D structure databases
ProteinModelPortal	A2SKR5.
ModBase	Search...
Protein-protein interaction databases
STRING	A2SKR5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4786540.
GenomeReviews	Gene locus Mpe_A3201 in contig CP000555_GR.
KEGG	mpt:Mpe_A3201.
NMPDR	fig|279263.3.peg.3822.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	FWRGVRP.
PhylomeDB	A2SKR5.
ProtClustDB	PRK00258.
Enzyme and pathway databases
BioCyc	MPET420662:MPE_A3201-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_METPP

Accession

Primary (citable) accession number: A2SKR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program