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A2SJR5

- TRMB_METPP

UniProt

A2SJR5 - TRMB_METPP

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Methylibium petroleiphilum (strain PM1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei287 – 2871S-adenosyl-L-methionineBy similarity
    Binding sitei312 – 3121S-adenosyl-L-methionineBy similarity
    Binding sitei339 – 3391S-adenosyl-L-methionineBy similarity
    Active sitei362 – 3621By similarity
    Binding sitei362 – 3621S-adenosyl-L-methionineBy similarity
    Binding sitei366 – 3661SubstrateBy similarity
    Binding sitei398 – 3981SubstrateBy similarity

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMPET420662:GHBE-2884-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:Mpe_A2850
    OrganismiMethylibium petroleiphilum (strain PM1)
    Taxonomic identifieri420662 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesMethylibium
    ProteomesiUP000000366: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469tRNA (guanine-N(7)-)-methyltransferasePRO_0000288178Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi420662.Mpe_A2850.

    Structurei

    3D structure databases

    ProteinModelPortaliA2SJR5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 221221UnknownAdd
    BLAST
    Regioni222 – 469248tRNA (guanine-N(7)-)-methyltransferaseAdd
    BLAST
    Regioni433 – 4364Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK03439.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A2SJR5-1 [UniParc]FASTAAdd to Basket

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    MRGGAHGPGD RAGRCREHRQ DHAGPCAGAG APFARPAGHA GGRVPARVLR    50
    RARPHAAARG TGRHRRRAMA SHRGRRAGRS HRAGRHHAAD DRGLQRVRVR 100
    RPQSLPPGAR APAPLRPHAA DRPRPGLAAR RPAARRRPGA RPGRPLGPRR 150
    AGLCRAALFG GLWRRLGAHG SRCGCAAPAA APARPGGPRA RRPAAAWPLP 200
    RMPAARMRTS VVRVRGEAPI AMSEPDSSSP PAHPPRPIRS FVLRAGRMGS 250
    GQTRALAELG PRFVLPFQRE PLDPQTVFGR RAPRVFEIGF GMGDATAQIA 300
    AARPDTDFIG CEVHTPGVGA LLKQIGEREL TNLRIVQHDA VEVLDHMIEP 350
    GSLAGIHLFF PDPWHKKKHH KRRLVQPAFV ERLVTRLAPG GYLHCATDWQ 400
    PYAEQMLEVL GAEPALRNSA DGYAPRPEYR PLTKFEQRGL RLGHGVWDLV 450
    FTKHQGRSAS KQCTATSSP 469
    Length:469
    Mass (Da):50,968
    Last modified:March 6, 2007 - v1
    Checksum:iE173CAD6CD3C08D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000555 Genomic DNA. Translation: ABM95804.1.
    RefSeqiYP_001022039.1. NC_008825.1.

    Genome annotation databases

    EnsemblBacteriaiABM95804; ABM95804; Mpe_A2850.
    GeneIDi4785544.
    KEGGimpt:Mpe_A2850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000555 Genomic DNA. Translation: ABM95804.1 .
    RefSeqi YP_001022039.1. NC_008825.1.

    3D structure databases

    ProteinModelPortali A2SJR5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 420662.Mpe_A2850.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM95804 ; ABM95804 ; Mpe_A2850 .
    GeneIDi 4785544.
    KEGGi mpt:Mpe_A2850.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K03439.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci MPET420662:GHBE-2884-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1."
      Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R.
      J. Bacteriol. 189:1931-1945(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PM1.

    Entry informationi

    Entry nameiTRMB_METPP
    AccessioniPrimary (citable) accession number: A2SJR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3