Reviewed, UniProtKB/Swiss-Prot A2SJJ0 (F16A2_METPP)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase class 1 2
      Short name=FBPase class 1 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 2

Gene names

Name:	fbp2
Ordered Locus Names:	Mpe_A2775

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Methylibium

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Carbohydrate biosynthesis; Calvin cycle. HAMAP MF_01855
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Calvin cycle Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Fructose-1,6-bisphosphatase class 1 2 HAMAP MF_01855		PRO_0000364597
Regions
	Region	117 – 120	4	Substrate binding By similarity
Sites
	Metal binding	92	1	Magnesium 1 By similarity
	Metal binding	114	1	Magnesium 1 By similarity
	Metal binding	114	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	117	1	Magnesium 2 By similarity
	Metal binding	281	1	Magnesium 2 By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SJJ0-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 4BBF9A63C8DD88F3	FASTA	336	36,989
	10 20 30 40 50 60 MTRRISLTQY LVEEQRERGR IPAQLRLLLE VVARACKSIA ISVNKGALGG VLGSAGSENV 70 80 90 100 110 120 QGEVQKKLDI IANEVLIEAN EWGGALAAMA SEEMDSIYVV PNRYPQGEYL LLFDPLDGSS 130 140 150 160 170 180 NIDVNVSIGT IFSVLKKQSD TPGVSTQDFL QPGSAQVAAG YCVYGPQTTL VLTVGDGVAM 190 200 210 220 230 240 FTLDREQGSW VLTTDEVRIP EDTREFAVNM SNQRHWAPPM QRYIDECLQG KDGPRGKDFN 250 260 270 280 290 300 MRWVASMVAD VHRILCRGGV FMYPWDKREP AKAGKLRLMY EANPMGFIVE QAGGAATNGK 310 320 330 QRVLDLQPSQ LHERVSVILG SKNEVERASA YHREAG

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed: 17158667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000555 Genomic DNA. Translation: ABM95729.1.
RefSeq	YP_001021964.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2SJJ0.
Genome annotation databases
GeneID	4784672.
GenomeReviews	Gene locus Mpe_A2775 in contig CP000555_GR.
KEGG	mpt:Mpe_A2775.
NMPDR	fig|279263.3.peg.3489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QRHWEAP.
Family and domain databases
HAMAP	MF_01855. [Tree]
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
ProDom	PD001491. In_FB_phphtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00124. FBPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16A2_METPP

Accession

Primary (citable) accession number: A2SJJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 6, 2007
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)